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- W2310520608 abstract "Lactic acid bacteria (LAB) isolated from Nham were screened for bacteriocin production by agar spot assay. Forty nine isolates of LAB were found to be able to inhibit Leuconostoc mesenteroides TISTR 473. Of all selected bacterial isolates, only one isolate, designated as NO5, harbored a plasmid of 2.5 kb in size. The bacterial isolate was identified as Lactococcus. LAB NO5 was tested for its inhibitory activity against a variety of indicator organism. It was found that the bacterial isolate inhibited only gram positive bacteria including Bacillus cereus ATCC 11778, Bacillus subtilis TISTR 008, Streptococcus pneumoniae DMS 5851, Streptococcus pyogenes ATCC 12384, Staphylococcus aureus ATCC 25923 and L. mesenteroides TISTR 473. By using scanning electron microscopy, no change was observed in cells of L. mesenteroides TISTR 473 treated with bacteriocin of LAB NO5. The bacteriocin was shown to be a protein with a molecular weight of approximately 10 kDa. It was resistant to heat treatment at 121 IC for at least 15 min. The highest bacteriocin activity 1,280 AU/ml, was detected after the culture of LAB NO5 was grown for 7 and 8 h. The localization of bacteriocin gene was also studied. It was found that the gene was likely to be located on the plasmid. §” ”§—≠ : ·≈§μ‘§·O ‘¥·∫§∑’‡√’¬ ae≈“ i‘¥" @default.
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- W2310520608 date "2007-01-01" @default.
- W2310520608 modified "2023-09-24" @default.
- W2310520608 title "§ÿ—''''â'''' ∑'â— Characterization of a Bacteriocin Produced by Lactic Acid Bacteria Isolated from Fermented Foods" @default.
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