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- W2311204069 abstract "The assessment of prokaryotic metabolic function in situ is challenged by the complexity of natural microbial communities, by the lack of information about the genetics of most environmental microbes, and the fact most prokaryotes are yet uncultured. Techniques that measure activity at the single cell level and simultaneously allow for taxonomic identification, despite being labor intensive, provide a window into a world once known only as the microbial black box. In this chapter, three of such approaches that combine microautoradiography with FISH are explored, with a focus on Substrate-Tracking Auto-Radiography Fluorescence In Situ Hybridization (STARFISH). The technical aspects of the protocols were summarized for a better understanding of the applications, their strengths and limitations. These techniques can quantitatively interrogate whether organisms of interest can metabolize particular substrates without the need of cultivation. Examples of various applications are presented. Advancement in high-throughput DNA sequencing technologies has quickly generated large amount of microbial genomic information. Techniques like STARFISH can be applied to validate in silico genetic predictions of microbial metabolic function." @default.
- W2311204069 created "2016-06-24" @default.
- W2311204069 creator A5008224911 @default.
- W2311204069 date "2015-09-01" @default.
- W2311204069 modified "2023-09-25" @default.
- W2311204069 title "Assessment of Prokaryotic Biological Activity at the Single-Cell Level by Combining Microautoradiography and Fluorescence in situ Hybridization" @default.
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- W2311204069 doi "https://doi.org/10.1128/9781555818821.ch2.2.2" @default.
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