Matches in SemOpenAlex for { <https://semopenalex.org/work/W2312808359> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2312808359 abstract "Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PABackground:CSCs are defined as minor cell population which have higher tumor-initiating ability, self-renewal ability and multilineage potential. Although CSCs in oral squamous cell carcinoma (OSCC) were identified by several surface antigen (e.g., CD44, CD133) or ALDEFLOUR assay, it is still elusive which are universal markers for CSC in OSCC. The aim of this study is to clarify potential CSC markers for a OSCC cell line using a combination of cell surface markers, CD44v3 and CD24.Materials and Methods: A OSCC cell line, SAS, was used in the experiment. CD44v3+/CD24− cell fraction was sorted from SAS cells with a FACSAria II and compared with the other three fractions (CD44v3+/CD24+, CD44v3− /CD24−, CD44v3−/CD24+ cell fraction) in cell proliferation, drug resistance and sphere forming ability. mRNA expression levels of various genes, such as stemness genes, anti-apoptosis genes and hypoxia-related genes, were compared among the fractions using quantitative real-time reverse transcriptase-polymerase chain reaction.Results:The proportion of CD44v3+/CD24−, CD44v3+/CD24+, CD44v3−/CD24−, CD44v3−/CD24+ cells was 34.9%, 39.8%, 14.2% and 11.0%, respectively. CD44v3+/CD24− cells showed a higher sphere forming ability, and drug resistance for CDDP, 5-FU and Cetuximab and expressed higher mRNA levels of CSC property-related genes (ABCG2, Oct-4, Nanog) and a hypoxia-related gene (HIF-1α) than the other cell fractions. There were no significant differences in cell proliferation among the fractions.Conclusion:The results suggest that CD44v3+/CD24− cell fractions in SAS possess CSC-like properties. The investigation of tumorigenic capacity in NOD/SCID mice is now under way.Citation Format: Keita Todoroki, Sachiko Ogasawara, Jun Akiba, Masamichi Nakayama, Yoshiki Naito, Jingo Kusukawa, Hirohisa Yano. CD44v3-positive (CD44v3+) and CD24-negative (CD24−) cells possess cancer stem cell (CSC)-like properties in a human oral squamous cell carcinoma cell line. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 1393. doi:10.1158/1538-7445.AM2015-1393" @default.
- W2312808359 created "2016-06-24" @default.
- W2312808359 creator A5021957719 @default.
- W2312808359 creator A5024527863 @default.
- W2312808359 creator A5037397895 @default.
- W2312808359 creator A5054240782 @default.
- W2312808359 creator A5062402333 @default.
- W2312808359 creator A5071892047 @default.
- W2312808359 creator A5081172876 @default.
- W2312808359 date "2015-08-01" @default.
- W2312808359 modified "2023-09-25" @default.
- W2312808359 title "Abstract 1393: CD44v3-positive (CD44v3+) and CD24-negative (CD24−) cells possess cancer stem cell (CSC)-like properties in a human oral squamous cell carcinoma cell line" @default.
- W2312808359 doi "https://doi.org/10.1158/1538-7445.am2015-1393" @default.
- W2312808359 hasPublicationYear "2015" @default.
- W2312808359 type Work @default.
- W2312808359 sameAs 2312808359 @default.
- W2312808359 citedByCount "0" @default.
- W2312808359 crossrefType "proceedings-article" @default.
- W2312808359 hasAuthorship W2312808359A5021957719 @default.
- W2312808359 hasAuthorship W2312808359A5024527863 @default.
- W2312808359 hasAuthorship W2312808359A5037397895 @default.
- W2312808359 hasAuthorship W2312808359A5054240782 @default.
- W2312808359 hasAuthorship W2312808359A5062402333 @default.
- W2312808359 hasAuthorship W2312808359A5071892047 @default.
- W2312808359 hasAuthorship W2312808359A5081172876 @default.
- W2312808359 hasConcept C104317684 @default.
- W2312808359 hasConcept C107459253 @default.
- W2312808359 hasConcept C145103041 @default.
- W2312808359 hasConcept C1491633281 @default.
- W2312808359 hasConcept C203014093 @default.
- W2312808359 hasConcept C207701140 @default.
- W2312808359 hasConcept C2779492189 @default.
- W2312808359 hasConcept C2780932548 @default.
- W2312808359 hasConcept C28328180 @default.
- W2312808359 hasConcept C2908647359 @default.
- W2312808359 hasConcept C502942594 @default.
- W2312808359 hasConcept C54355233 @default.
- W2312808359 hasConcept C55427017 @default.
- W2312808359 hasConcept C71924100 @default.
- W2312808359 hasConcept C81885089 @default.
- W2312808359 hasConcept C86803240 @default.
- W2312808359 hasConcept C95444343 @default.
- W2312808359 hasConcept C99454951 @default.
- W2312808359 hasConceptScore W2312808359C104317684 @default.
- W2312808359 hasConceptScore W2312808359C107459253 @default.
- W2312808359 hasConceptScore W2312808359C145103041 @default.
- W2312808359 hasConceptScore W2312808359C1491633281 @default.
- W2312808359 hasConceptScore W2312808359C203014093 @default.
- W2312808359 hasConceptScore W2312808359C207701140 @default.
- W2312808359 hasConceptScore W2312808359C2779492189 @default.
- W2312808359 hasConceptScore W2312808359C2780932548 @default.
- W2312808359 hasConceptScore W2312808359C28328180 @default.
- W2312808359 hasConceptScore W2312808359C2908647359 @default.
- W2312808359 hasConceptScore W2312808359C502942594 @default.
- W2312808359 hasConceptScore W2312808359C54355233 @default.
- W2312808359 hasConceptScore W2312808359C55427017 @default.
- W2312808359 hasConceptScore W2312808359C71924100 @default.
- W2312808359 hasConceptScore W2312808359C81885089 @default.
- W2312808359 hasConceptScore W2312808359C86803240 @default.
- W2312808359 hasConceptScore W2312808359C95444343 @default.
- W2312808359 hasConceptScore W2312808359C99454951 @default.
- W2312808359 hasLocation W23128083591 @default.
- W2312808359 hasOpenAccess W2312808359 @default.
- W2312808359 hasPrimaryLocation W23128083591 @default.
- W2312808359 hasRelatedWork W1531653295 @default.
- W2312808359 hasRelatedWork W1561935643 @default.
- W2312808359 hasRelatedWork W2000255540 @default.
- W2312808359 hasRelatedWork W2004124473 @default.
- W2312808359 hasRelatedWork W2051774072 @default.
- W2312808359 hasRelatedWork W2073740148 @default.
- W2312808359 hasRelatedWork W2101497946 @default.
- W2312808359 hasRelatedWork W2138143726 @default.
- W2312808359 hasRelatedWork W2236447015 @default.
- W2312808359 hasRelatedWork W2379824762 @default.
- W2312808359 hasRelatedWork W2411585626 @default.
- W2312808359 hasRelatedWork W2497999604 @default.
- W2312808359 hasRelatedWork W2551479680 @default.
- W2312808359 hasRelatedWork W2620558854 @default.
- W2312808359 hasRelatedWork W2622701289 @default.
- W2312808359 hasRelatedWork W2740858695 @default.
- W2312808359 hasRelatedWork W2741946367 @default.
- W2312808359 hasRelatedWork W2764126878 @default.
- W2312808359 hasRelatedWork W3158989825 @default.
- W2312808359 hasRelatedWork W86546573 @default.
- W2312808359 isParatext "false" @default.
- W2312808359 isRetracted "false" @default.
- W2312808359 magId "2312808359" @default.
- W2312808359 workType "article" @default.