Matches in SemOpenAlex for { <https://semopenalex.org/work/W2312912596> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2312912596 abstract "Hypoxic microenvironment and overexpression of choline kinase alpha (ChKa) have been associated with tumor malignancy, but the regulation of ChKa expression in hypoxic conditions is poorly understood. In this study, the effects of chronic hypoxia on choline metabolism and ChKa expression were examined in prostate cancer cell line, PC-3. Chronic hypoxia (1% O2, 24h) decreased proliferation rate and intracellular levels of phosphocholine, while intracellular levels of free choline were increased. Choline tracer uptake and washout studies confirmed the decreased rate of choline phosphorylation in hypoxic cells. Overexpression of HIF1a or DMOG exposure in normoxic PC-3 cells decreased both choline uptake and choline kinase activity. RT-PCR analysis showed decreased transcription of ChKa gene in hypoxic PC-3 correlating with decreased enzymatic activity of choline kinase in hypoxic cell extracts. Alignment of nucleotide sequence of the putative promoter region of ChKa from rat, mouse, chimpanzee and human revealed a highly conserved hypoxia responsive element upstream to transcription start site of ChKa. Electrophoretic mobility shift competition/supershift assay and chromatin immunoprecipitation-quantitative PCR assay confirmed binding of native HIF-1α protein to the conserved HRE. To understand the transcription control of ChKa under hypoxia, a putative promoter of ChKa was isolated from PC-3 genomic DNA and cloned into a luciferase (Luc) based reporter vector system. In PC-3, hypoxia decreased the expression of Luc under the control of the ChKa promoter. Mutation of the conserved HRE and subsequent Luc reporter assays demonstrated that this HRE is involved in hypoxia/HIF1α mediated regulation of ChKa. The results suggest that the decreased choline phosphorylation in PC-3 cells in response to hypoxia is mediated in part by the newly-identified conserved HIF1α binding HRE. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-377." @default.
- W2312912596 created "2016-06-24" @default.
- W2312912596 creator A5013482505 @default.
- W2312912596 creator A5040861278 @default.
- W2312912596 creator A5086617626 @default.
- W2312912596 date "2010-04-15" @default.
- W2312912596 modified "2023-09-25" @default.
- W2312912596 title "Abstract LB-377: Regulation of expression of choline kinase by a conserved HIF1 binding hypoxia-responsive element" @default.
- W2312912596 doi "https://doi.org/10.1158/1538-7445.am10-lb-377" @default.
- W2312912596 hasPublicationYear "2010" @default.
- W2312912596 type Work @default.
- W2312912596 sameAs 2312912596 @default.
- W2312912596 citedByCount "0" @default.
- W2312912596 crossrefType "proceedings-article" @default.
- W2312912596 hasAuthorship W2312912596A5013482505 @default.
- W2312912596 hasAuthorship W2312912596A5040861278 @default.
- W2312912596 hasAuthorship W2312912596A5086617626 @default.
- W2312912596 hasConcept C101762097 @default.
- W2312912596 hasConcept C104317684 @default.
- W2312912596 hasConcept C134320426 @default.
- W2312912596 hasConcept C150194340 @default.
- W2312912596 hasConcept C153911025 @default.
- W2312912596 hasConcept C184235292 @default.
- W2312912596 hasConcept C2776330855 @default.
- W2312912596 hasConcept C2776923060 @default.
- W2312912596 hasConcept C2778918659 @default.
- W2312912596 hasConcept C2780601828 @default.
- W2312912596 hasConcept C2781123555 @default.
- W2312912596 hasConcept C41625074 @default.
- W2312912596 hasConcept C55493867 @default.
- W2312912596 hasConcept C86339819 @default.
- W2312912596 hasConcept C86803240 @default.
- W2312912596 hasConceptScore W2312912596C101762097 @default.
- W2312912596 hasConceptScore W2312912596C104317684 @default.
- W2312912596 hasConceptScore W2312912596C134320426 @default.
- W2312912596 hasConceptScore W2312912596C150194340 @default.
- W2312912596 hasConceptScore W2312912596C153911025 @default.
- W2312912596 hasConceptScore W2312912596C184235292 @default.
- W2312912596 hasConceptScore W2312912596C2776330855 @default.
- W2312912596 hasConceptScore W2312912596C2776923060 @default.
- W2312912596 hasConceptScore W2312912596C2778918659 @default.
- W2312912596 hasConceptScore W2312912596C2780601828 @default.
- W2312912596 hasConceptScore W2312912596C2781123555 @default.
- W2312912596 hasConceptScore W2312912596C41625074 @default.
- W2312912596 hasConceptScore W2312912596C55493867 @default.
- W2312912596 hasConceptScore W2312912596C86339819 @default.
- W2312912596 hasConceptScore W2312912596C86803240 @default.
- W2312912596 hasLocation W23129125961 @default.
- W2312912596 hasOpenAccess W2312912596 @default.
- W2312912596 hasPrimaryLocation W23129125961 @default.
- W2312912596 hasRelatedWork W1550629899 @default.
- W2312912596 hasRelatedWork W1971279082 @default.
- W2312912596 hasRelatedWork W1972783950 @default.
- W2312912596 hasRelatedWork W1985129279 @default.
- W2312912596 hasRelatedWork W2007919022 @default.
- W2312912596 hasRelatedWork W2035049657 @default.
- W2312912596 hasRelatedWork W2065697804 @default.
- W2312912596 hasRelatedWork W2073797382 @default.
- W2312912596 hasRelatedWork W2081635255 @default.
- W2312912596 hasRelatedWork W2090267337 @default.
- W2312912596 hasRelatedWork W2116763746 @default.
- W2312912596 hasRelatedWork W2126592654 @default.
- W2312912596 hasRelatedWork W2128666034 @default.
- W2312912596 hasRelatedWork W2128749280 @default.
- W2312912596 hasRelatedWork W2140353112 @default.
- W2312912596 hasRelatedWork W2165235603 @default.
- W2312912596 hasRelatedWork W2168736084 @default.
- W2312912596 hasRelatedWork W2339606698 @default.
- W2312912596 hasRelatedWork W2808193471 @default.
- W2312912596 hasRelatedWork W2503543377 @default.
- W2312912596 isParatext "false" @default.
- W2312912596 isRetracted "false" @default.
- W2312912596 magId "2312912596" @default.
- W2312912596 workType "article" @default.