FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2313078897> ?p ?o ?g. }

• W2313078897 abstract "Introduction and Objective: We recently demonstrated that TGF-β mediated phosphorylation of extra-cellular signal-regulated kinase (ERK) activation results in the prostate cancer (CaP) progression and metastasis. Serine/threonine protein phosphatases 2, PP2A (including subunit −A, −B and −C) are well known to be involved in the dephosphorylation and inactivation of ERK. In this study, we determined the association between the recruitment of PP2A by TGF-β receptors (TβRI and TβRII) and activation of ERK under the treatment of TGF-β. Methods: The human CaP cell lines PC-3 with different capability of aggressive (PC-3, PC-3M, PC-3M-Pro and PC-3M-LN4), and benign prostate epithelial cell line BPH-1 were used for these studies. Cells were treated with TGF-β (10ng/mL) for 24 minutes. The expression of phospho-ERK (p-ERK), total-ERK (t-ERK), TβRs was evaluated by quantitative western blot analyses. The conjugation of PP2A (−A, −B and −C) and TβRI and TβRII were elucidated using western blot following immunoprecipitation (IP, Pierce Crosslink kit) with TβRI and TβRII as the precipitant respectively. Briefly, precleared lysate was immunopreciptated by the crosslinked TβRI or TβRII antibody (5 μg) and agarose mixture for overnight on 4°C. Control agarose resin in the kit was used as a negative control when western-blot for PP2A was conducted. The recruitment of PP2A by TβRs was correlated with the expression of p-ERK and TβRs. Results: TGF-s treatment resulted in an increase in p-Erk expression (4-fold) in all PC-3 cell lines in a time dependent manner post TGF-s exposure. In addition, the expressions of TsRI and TsRII were suppressed by 46% and 29% respectively. IP studies revealed recruitment of PP2A conjugated with TsRI and TsRII. In contrast, the expression of p-Erk was dramatically inhibited in BPH-1 by TGF-s exposure. Although there is no significant change on the expression of TsRs, the ratio of PP2A versus TsRII was significantly increased from 2.08 to 3.12, which suggests that the recruitment of PP2A was relatively increased in BPH-1 cells under the treatment of TGF-s. Finally, there was a reverse correlation between recruited PP2A and activation of p-ERK in both PC-3 cell lines, and BPH-1 cells. Conclusion: Taken together the results suggest that TGF-β suppresses the recruitment of PP2A by TGF-β receptors in CaP cells in contrast to benign prostate cells, which results in relatively increased activation of ERK and the subsequent tumor progression. The identification of the recruitment of PP2A by TβRs represents a new focus to elucidate in part how TGF-β plays a different, or even a contrary role in CaP and benign cell respectively. PP2A may be a potential new target for CaP therapies. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-3. doi:10.1158/1538-7445.AM2011-LB-3" @default.
• W2313078897 created "2016-06-24" @default.
• W2313078897 creator A5003177689 @default.
• W2313078897 creator A5019669673 @default.
• W2313078897 creator A5022510502 @default.
• W2313078897 creator A5030411648 @default.
• W2313078897 creator A5039837606 @default.
• W2313078897 creator A5073389579 @default.
• W2313078897 creator A5078143614 @default.
• W2313078897 date "2011-04-15" @default.
• W2313078897 modified "2023-09-25" @default.
• W2313078897 title "Abstract LB-3: The recruitment of PP2A by TGF-β receptors mediates the response to TGF-β-induced activation of ERK in prostate cancer" @default.
• W2313078897 doi "https://doi.org/10.1158/1538-7445.am2011-lb-3" @default.
• W2313078897 hasPublicationYear "2011" @default.
• W2313078897 type Work @default.
• W2313078897 sameAs 2313078897 @default.
• W2313078897 citedByCount "1" @default.
• W2313078897 crossrefType "proceedings-article" @default.
• W2313078897 hasAuthorship W2313078897A5003177689 @default.
• W2313078897 hasAuthorship W2313078897A5019669673 @default.
• W2313078897 hasAuthorship W2313078897A5022510502 @default.
• W2313078897 hasAuthorship W2313078897A5030411648 @default.
• W2313078897 hasAuthorship W2313078897A5039837606 @default.
• W2313078897 hasAuthorship W2313078897A5073389579 @default.
• W2313078897 hasAuthorship W2313078897A5078143614 @default.
• W2313078897 hasConcept C104317684 @default.
• W2313078897 hasConcept C11960822 @default.
• W2313078897 hasConcept C121608353 @default.
• W2313078897 hasConcept C126322002 @default.
• W2313078897 hasConcept C153911025 @default.
• W2313078897 hasConcept C170493617 @default.
• W2313078897 hasConcept C178666793 @default.
• W2313078897 hasConcept C181912034 @default.
• W2313078897 hasConcept C184235292 @default.
• W2313078897 hasConcept C185592680 @default.
• W2313078897 hasConcept C2776415932 @default.
• W2313078897 hasConcept C2780192828 @default.
• W2313078897 hasConcept C389152 @default.
• W2313078897 hasConcept C502942594 @default.
• W2313078897 hasConcept C55493867 @default.
• W2313078897 hasConcept C57074206 @default.
• W2313078897 hasConcept C71924100 @default.
• W2313078897 hasConcept C86803240 @default.
• W2313078897 hasConcept C95444343 @default.
• W2313078897 hasConceptScore W2313078897C104317684 @default.
• W2313078897 hasConceptScore W2313078897C11960822 @default.
• W2313078897 hasConceptScore W2313078897C121608353 @default.
• W2313078897 hasConceptScore W2313078897C126322002 @default.
• W2313078897 hasConceptScore W2313078897C153911025 @default.
• W2313078897 hasConceptScore W2313078897C170493617 @default.
• W2313078897 hasConceptScore W2313078897C178666793 @default.
• W2313078897 hasConceptScore W2313078897C181912034 @default.
• W2313078897 hasConceptScore W2313078897C184235292 @default.
• W2313078897 hasConceptScore W2313078897C185592680 @default.
• W2313078897 hasConceptScore W2313078897C2776415932 @default.
• W2313078897 hasConceptScore W2313078897C2780192828 @default.
• W2313078897 hasConceptScore W2313078897C389152 @default.
• W2313078897 hasConceptScore W2313078897C502942594 @default.
• W2313078897 hasConceptScore W2313078897C55493867 @default.
• W2313078897 hasConceptScore W2313078897C57074206 @default.
• W2313078897 hasConceptScore W2313078897C71924100 @default.
• W2313078897 hasConceptScore W2313078897C86803240 @default.
• W2313078897 hasConceptScore W2313078897C95444343 @default.
• W2313078897 hasLocation W23130788971 @default.
• W2313078897 hasOpenAccess W2313078897 @default.
• W2313078897 hasPrimaryLocation W23130788971 @default.
• W2313078897 hasRelatedWork W1522700610 @default.
• W2313078897 hasRelatedWork W1982886131 @default.
• W2313078897 hasRelatedWork W2018984807 @default.
• W2313078897 hasRelatedWork W2068098738 @default.
• W2313078897 hasRelatedWork W2133084632 @default.
• W2313078897 hasRelatedWork W2183298799 @default.
• W2313078897 hasRelatedWork W2351330689 @default.
• W2313078897 hasRelatedWork W2357873406 @default.
• W2313078897 hasRelatedWork W2408624373 @default.
• W2313078897 hasRelatedWork W2573280318 @default.
• W2313078897 hasRelatedWork W2582391379 @default.
• W2313078897 hasRelatedWork W2888693977 @default.
• W2313078897 hasRelatedWork W2996490225 @default.
• W2313078897 hasRelatedWork W3006209933 @default.
• W2313078897 hasRelatedWork W3014355282 @default.
• W2313078897 hasRelatedWork W3025701927 @default.
• W2313078897 hasRelatedWork W3025948000 @default.
• W2313078897 hasRelatedWork W3030425960 @default.
• W2313078897 hasRelatedWork W3094003798 @default.
• W2313078897 hasRelatedWork W4400583 @default.
• W2313078897 isParatext "false" @default.
• W2313078897 isRetracted "false" @default.
• W2313078897 magId "2313078897" @default.
• W2313078897 workType "article" @default.