FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2313132043> ?p ?o ?g. }

• W2313132043 endingPage "16183" @default.
• W2313132043 startingPage "16171" @default.
• W2313132043 abstract "Rat β-parvalbumin (β-PV) and chicken parvalbumin 3 (CPV3) exhibit diminished Ca2+ affinity. Their sequences, 70% identical, are unusual in that serine replaces the consensus residue, valine, at position 33. Reasoning that the substitution of a compact, polar hydroxymethyl moiety for a bulky, apolar isopropyl group might contribute to the attenuated Ca2+ affinities, we have characterized the S33V variants of both proteins. The impact of the mutation in CPV3 differs decidedly from that in rat β. Whereas replacement of S33 by valine in CPV3 causes a substantial increase in the solvent-accessible apolar surface in the Ca2+-free protein, the mutation evidently decreases the exposed apolar surface area in rat β. Although the mutation has a minimal effect on divalent ion affinity in both proteins, the ΔΔH and −TΔΔS changes for Ca2+ binding in CPV3 S33V, but not rat β S33V, are consistent with increased burial of the apolar surface. The influence of the S33V substitution on conformational stability likewise differs for rat β-PV and CPV3. Whereas the stability of the former is virtually unperturbed by the sequence alteration, the latter is destabilized by 0.7 kcal/mol. Moreover, the mutation greatly exacerbates the tendency for CPV3 to aggregate. The concentration and scan rate dependence observed in DSC studies of CPV3 S33V denaturation suggest that unfolding proceeds through an intermediate state that is prone to aggregation. Consistent with this idea, reversible unfolding data, collected at very low protein concentration, likewise indicate that the thermal denaturation is not a two-state process." @default.
• W2313132043 created "2016-06-24" @default.
• W2313132043 creator A5012011291 @default.
• W2313132043 creator A5041179608 @default.
• W2313132043 creator A5059637670 @default.
• W2313132043 date "2010-10-20" @default.
• W2313132043 modified "2023-09-26" @default.
• W2313132043 title "Disparate Impact of the S33V Mutation on Conformational Stability in Rat β-Parvalbumin (Oncomodulin) and Chicken Parvalbumin 3" @default.
• W2313132043 cites W1505902561 @default.
• W2313132043 cites W1547655165 @default.
• W2313132043 cites W1565342485 @default.
• W2313132043 cites W1570865922 @default.
• W2313132043 cites W1571243084 @default.
• W2313132043 cites W1599096372 @default.
• W2313132043 cites W1645871876 @default.
• W2313132043 cites W1745759832 @default.
• W2313132043 cites W1971186417 @default.
• W2313132043 cites W1973067048 @default.
• W2313132043 cites W1978683984 @default.
• W2313132043 cites W1989826574 @default.
• W2313132043 cites W1991622845 @default.
• W2313132043 cites W1998347647 @default.
• W2313132043 cites W2001458612 @default.
• W2313132043 cites W2003757758 @default.
• W2313132043 cites W2011706602 @default.
• W2313132043 cites W2012369036 @default.
• W2313132043 cites W2024661068 @default.
• W2313132043 cites W2034077172 @default.
• W2313132043 cites W2038263678 @default.
• W2313132043 cites W2038909177 @default.
• W2313132043 cites W2039366113 @default.
• W2313132043 cites W2049416967 @default.
• W2313132043 cites W2049649579 @default.
• W2313132043 cites W2053897303 @default.
• W2313132043 cites W2069848747 @default.
• W2313132043 cites W2070785831 @default.
• W2313132043 cites W2073834370 @default.
• W2313132043 cites W2080186206 @default.
• W2313132043 cites W2088206984 @default.
• W2313132043 cites W2088327575 @default.
• W2313132043 cites W2089563549 @default.
• W2313132043 cites W2096716204 @default.
• W2313132043 cites W2116076986 @default.
• W2313132043 cites W2134550847 @default.
• W2313132043 cites W2138825060 @default.
• W2313132043 cites W2156731158 @default.
• W2313132043 cites W2158770981 @default.
• W2313132043 cites W2175913572 @default.
• W2313132043 doi "https://doi.org/10.1021/jp1063325" @default.
• W2313132043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20961100" @default.
• W2313132043 hasPublicationYear "2010" @default.
• W2313132043 type Work @default.
• W2313132043 sameAs 2313132043 @default.
• W2313132043 citedByCount "1" @default.
• W2313132043 countsByYear W23131320432017 @default.
• W2313132043 crossrefType "journal-article" @default.
• W2313132043 hasAuthorship W2313132043A5012011291 @default.
• W2313132043 hasAuthorship W2313132043A5041179608 @default.
• W2313132043 hasAuthorship W2313132043A5059637670 @default.
• W2313132043 hasConcept C12554922 @default.
• W2313132043 hasConcept C13965031 @default.
• W2313132043 hasConcept C141547260 @default.
• W2313132043 hasConcept C185592680 @default.
• W2313132043 hasConcept C2776923230 @default.
• W2313132043 hasConcept C2777573094 @default.
• W2313132043 hasConcept C515207424 @default.
• W2313132043 hasConcept C54355233 @default.
• W2313132043 hasConcept C55493867 @default.
• W2313132043 hasConcept C8010536 @default.
• W2313132043 hasConcept C86803240 @default.
• W2313132043 hasConceptScore W2313132043C12554922 @default.
• W2313132043 hasConceptScore W2313132043C13965031 @default.
• W2313132043 hasConceptScore W2313132043C141547260 @default.
• W2313132043 hasConceptScore W2313132043C185592680 @default.
• W2313132043 hasConceptScore W2313132043C2776923230 @default.
• W2313132043 hasConceptScore W2313132043C2777573094 @default.
• W2313132043 hasConceptScore W2313132043C515207424 @default.
• W2313132043 hasConceptScore W2313132043C54355233 @default.
• W2313132043 hasConceptScore W2313132043C55493867 @default.
• W2313132043 hasConceptScore W2313132043C8010536 @default.
• W2313132043 hasConceptScore W2313132043C86803240 @default.
• W2313132043 hasIssue "49" @default.
• W2313132043 hasLocation W23131320431 @default.
• W2313132043 hasLocation W23131320432 @default.
• W2313132043 hasOpenAccess W2313132043 @default.
• W2313132043 hasPrimaryLocation W23131320431 @default.
• W2313132043 hasRelatedWork W1975952480 @default.
• W2313132043 hasRelatedWork W1996453392 @default.
• W2313132043 hasRelatedWork W2024305546 @default.
• W2313132043 hasRelatedWork W2033776077 @default.
• W2313132043 hasRelatedWork W2058900218 @default.
• W2313132043 hasRelatedWork W2098918811 @default.
• W2313132043 hasRelatedWork W2159714739 @default.
• W2313132043 hasRelatedWork W2198982186 @default.
• W2313132043 hasRelatedWork W2416727678 @default.
• W2313132043 hasRelatedWork W2100132523 @default.
• W2313132043 hasVolume "114" @default.
• W2313132043 isParatext "false" @default.

• next