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- W2313142787 abstract "One of the earliest and largest transcriptional responses that occur during exposure of Synechocystis sp. PCC6803 to cold is the induction of the crhR RNA helicase transcript. We show that crhR deletion results in failure to cold acclimate: there is reduced growth at 24 °C and marked impairment of growth at 20 °C. 2D-DIGE, using five biological replicates, was used to analyze the proteomic differences between the wild-type and ΔcrhR strains grown at (1) 34 °C and (2) following transfer from 34 to 24 °C (cold-acclimation). Sixteen significantly differentially expressed proteins were identified between the two strains grown at 34 °C. Forty-three distinct proteins were identified that responded to cold-acclimation of the wild-type and 34 proteins for the mutant, with only 26 proteins common to both. A large proportion of the proteomic responses (76.5%) could not be predicted from published transcriptomic data. Only modest similarity is observed between proteomic and transcriptomic responses (r = 0.54-0.70). We propose functions for three previously hypothetical proteins. We suggest molecular targets for CrhR action and identify downstream regulated events in metabolism." @default.
- W2313142787 created "2016-06-24" @default.
- W2313142787 creator A5022313002 @default.
- W2313142787 creator A5025300568 @default.
- W2313142787 creator A5055782961 @default.
- W2313142787 creator A5076752152 @default.
- W2313142787 date "2011-07-08" @default.
- W2313142787 modified "2023-10-18" @default.
- W2313142787 title "Proteomics Reveals a Role for the RNA Helicase <i>crhR</i> in the Modulation of Multiple Metabolic Pathways during Cold Acclimation of <i>Synechocystis</i> sp. PCC6803" @default.
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- W2313142787 doi "https://doi.org/10.1021/pr200299t" @default.
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