FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2313248048> ?p ?o ?g. }

• W2313248048 endingPage "16176" @default.
• W2313248048 startingPage "16167" @default.
• W2313248048 abstract "In the present study, the complexation between linear 13.4 kDa poly(ethylene imine) (LPEI) and plasmid DNA was investigated. Analytical ultracentrifugation (AUC) was used for size and molar mass determination. Additionally, the morphology was studied by scanning force microscopy. The polyplex formation was investigated in a wide range of PEI nitrogen to DNA phosphate ratios (N/P). At N/P ratios below 1, the PEI/DNA complex formation is characterized by an incomplete DNA condensation and the formation of the primary DNA/PEI complexes. The merging of the initially formed polyplexes occurs at N/P ~2, resulting in the formation of polyplexes with much larger size and high aggregation rate. Stable and uniform polyplexes were formed at N/P > 10, with average sizes of the polyplexes of about 170 ± 65 nm. The content of uncomplexed PEI chains in the polyplex dispersion was estimated at four different N/P ratios, 6.2, 11.6, 28.6, and 57.8, by combining preparative centrifugation with a copper complex assay and by sedimentation velocity analysis as an alternative method. It is demonstrated that virtually all added PEI binds to the DNA at N/P < 2.5; further addition of PEI results in the appearance of a large amount of free PEI in solution. Nevertheless, PEI is able to bind in the whole range of N/P ratios tested. According to the data collected by sedimentation velocity analysis and scanning force microscopy, the single PEI/DNA complexes are composed on average of 8 to 32 single condensed DNA plasmids and 70 ± 25 PEI molecules." @default.
• W2313248048 created "2016-06-24" @default.
• W2313248048 creator A5023649331 @default.
• W2313248048 creator A5032037925 @default.
• W2313248048 creator A5032562861 @default.
• W2313248048 creator A5034341324 @default.
• W2313248048 creator A5071910582 @default.
• W2313248048 creator A5075012306 @default.
• W2313248048 creator A5091635077 @default.
• W2313248048 date "2012-11-05" @default.
• W2313248048 modified "2023-09-26" @default.
• W2313248048 title "Polyelectrolyte Complexes of DNA and Linear PEI: Formation, Composition and Properties" @default.
• W2313248048 cites W1540064031 @default.
• W2313248048 cites W1560355462 @default.
• W2313248048 cites W1580847663 @default.
• W2313248048 cites W1964523057 @default.
• W2313248048 cites W1968433618 @default.
• W2313248048 cites W1970594627 @default.
• W2313248048 cites W1973579525 @default.
• W2313248048 cites W1975317507 @default.
• W2313248048 cites W1981045116 @default.
• W2313248048 cites W1984739176 @default.
• W2313248048 cites W1991300442 @default.
• W2313248048 cites W1993081501 @default.
• W2313248048 cites W1996724063 @default.
• W2313248048 cites W2002151233 @default.
• W2313248048 cites W2003414091 @default.
• W2313248048 cites W2009508594 @default.
• W2313248048 cites W2010655741 @default.
• W2313248048 cites W2013168545 @default.
• W2313248048 cites W2025857959 @default.
• W2313248048 cites W2050932091 @default.
• W2313248048 cites W2054532423 @default.
• W2313248048 cites W2055341529 @default.
• W2313248048 cites W2069990491 @default.
• W2313248048 cites W2070794824 @default.
• W2313248048 cites W2071476696 @default.
• W2313248048 cites W2078617396 @default.
• W2313248048 cites W2080071319 @default.
• W2313248048 cites W2087103327 @default.
• W2313248048 cites W2087497808 @default.
• W2313248048 cites W2110964550 @default.
• W2313248048 cites W2112073236 @default.
• W2313248048 cites W2127021351 @default.
• W2313248048 cites W2127685088 @default.
• W2313248048 cites W2129569835 @default.
• W2313248048 cites W2141778672 @default.
• W2313248048 cites W2142987628 @default.
• W2313248048 cites W2145267183 @default.
• W2313248048 cites W2160039306 @default.
• W2313248048 cites W2163792954 @default.
• W2313248048 cites W2164895189 @default.
• W2313248048 cites W2279794708 @default.
• W2313248048 cites W2322237608 @default.
• W2313248048 cites W304669699 @default.
• W2313248048 cites W4233309778 @default.
• W2313248048 doi "https://doi.org/10.1021/la303094b" @default.
• W2313248048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23083317" @default.
• W2313248048 hasPublicationYear "2012" @default.
• W2313248048 type Work @default.
• W2313248048 sameAs 2313248048 @default.
• W2313248048 citedByCount "64" @default.
• W2313248048 countsByYear W23132480482013 @default.
• W2313248048 countsByYear W23132480482014 @default.
• W2313248048 countsByYear W23132480482015 @default.
• W2313248048 countsByYear W23132480482016 @default.
• W2313248048 countsByYear W23132480482017 @default.
• W2313248048 countsByYear W23132480482018 @default.
• W2313248048 countsByYear W23132480482019 @default.
• W2313248048 countsByYear W23132480482020 @default.
• W2313248048 countsByYear W23132480482021 @default.
• W2313248048 countsByYear W23132480482022 @default.
• W2313248048 countsByYear W23132480482023 @default.
• W2313248048 crossrefType "journal-article" @default.
• W2313248048 hasAuthorship W2313248048A5023649331 @default.
• W2313248048 hasAuthorship W2313248048A5032037925 @default.
• W2313248048 hasAuthorship W2313248048A5032562861 @default.
• W2313248048 hasAuthorship W2313248048A5034341324 @default.
• W2313248048 hasAuthorship W2313248048A5071910582 @default.
• W2313248048 hasAuthorship W2313248048A5075012306 @default.
• W2313248048 hasAuthorship W2313248048A5091635077 @default.
• W2313248048 hasConcept C113196181 @default.
• W2313248048 hasConcept C1388644 @default.
• W2313248048 hasConcept C153356533 @default.
• W2313248048 hasConcept C178790620 @default.
• W2313248048 hasConcept C181199279 @default.
• W2313248048 hasConcept C185592680 @default.
• W2313248048 hasConcept C188027245 @default.
• W2313248048 hasConcept C43617362 @default.
• W2313248048 hasConcept C521977710 @default.
• W2313248048 hasConcept C552990157 @default.
• W2313248048 hasConcept C55493867 @default.
• W2313248048 hasConcept C7623868 @default.
• W2313248048 hasConcept C8010536 @default.
• W2313248048 hasConceptScore W2313248048C113196181 @default.
• W2313248048 hasConceptScore W2313248048C1388644 @default.
• W2313248048 hasConceptScore W2313248048C153356533 @default.
• W2313248048 hasConceptScore W2313248048C178790620 @default.

• next