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• W2313253712 endingPage "C1336" @default.
• W2313253712 startingPage "C1323" @default.
• W2313253712 abstract "The intracellular ion homeostasis in cockroach salivary acinar cells during salivation is not satisfactorily understood. This is mainly due to technical problems regarding strong tissue autofluorescence and ineffective ion concentration quantification. For minimizing these problems, we describe the successful application of two-photon (2P) microscopy partly in combination with fluorescence lifetime imaging microscopy (FLIM) to record intracellular Na + and Cl − concentrations ([Na + ] i , [Cl − ] i ) in cockroach salivary acinar cells. Quantitative 2P-FLIM Cl − measurements with the dye N-(ethoxycarbonylmethyl)-6-methoxy-quinolinium bromide indicate that the resting [Cl − ] i is 1.6 times above the Cl − electrochemical equilibrium but is not influenced by pharmacological inhibition of the Na + -K + -2Cl − cotransporter (NKCC) and anion exchanger using bumetanide and 4,4′-diisothiocyanatodihydrostilbene-2,2′-disulfonic acid disodium salt. In contrast, rapid Cl − reuptake after extracellular Cl − removal is almost totally NKCC mediated both in the absence and presence of dopamine. However, in physiological saline [Cl − ] i does not change during dopamine stimulation although dopamine stimulates fluid secretion in these glands. On the other hand, dopamine causes a decrease in the sodium-binding benzofuran isophthalate tetra-ammonium salt (SBFI) fluorescence and an increase in the Sodium Green fluorescence after 2P excitation. This opposite behavior of both dyes suggests a dopamine-induced [Na + ] i rise in the acinar cells, which is supported by the determined 2P-action cross sections of SBFI. The [Na + ] i rise is Cl − dependent and inhibited by bumetanide. The Ca 2+ -ionophore ionomycin also causes a bumetanide-sensitive [Na + ] i rise. We propose that a Ca 2+ -mediated NKCC activity in acinar peripheral cells attributable to dopamine stimulation serves for basolateral Na + uptake during saliva secretion and that the concomitantly transported Cl − is recycled back to the bath." @default.
• W2313253712 created "2016-06-24" @default.
• W2313253712 creator A5005667504 @default.
• W2313253712 creator A5008790986 @default.
• W2313253712 creator A5047077086 @default.
• W2313253712 date "2011-06-01" @default.
• W2313253712 modified "2023-10-18" @default.
• W2313253712 title "Two-photon microscopy and fluorescence lifetime imaging reveal stimulus-induced intracellular Na⁺and Cl⁻changes in cockroach salivary acinar cells" @default.
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• W2313253712 doi "https://doi.org/10.1152/ajpcell.00320.2010" @default.
• W2313253712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21346157" @default.
• W2313253712 hasPublicationYear "2011" @default.
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