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- W2313385488 abstract "Premise of research. The pollen wall, arguably the most complex cell wall found in plants, is especially elaborate in the family Compositae. Through a detailed ontogenetic study of Tanacetum vulgare pollen, capturing each stage of development at a high level of resolution, we aim to understand the establishment of complex pollen walls and try to unravel the mechanisms and interactions underlying sporoderm development. Other aims are to compare our observations and interpretation with previous studies designed to elucidate the underlying mechanisms of sporoderm development and pattern determination in flowering plants and to clarify whether our earlier self-assembly hypothesis is applicable in Tanacetum.Methodology. TEM, SEM, chemical degradation, and comparative methods were used in this study.Pivotal results. The sequence of events leading to exine emergence from early tetrad stage to maturity is as follows: the appearance of spherical units, small invagination beneath spherical units, columns of spherical units, rodlike units (the future subtectal columellae), and a new layer of spacer matrix between rodlike units; growth of the inner columellae; dispersion of spacer matrix; sporopollenin accumulation on primexine; and deposition of lamellate endexine. Our observations are consistent with the sequence of self-assembling micellar mesophases.Conclusions. The microspore tetrad period is confirmed to be the most important stage, in which the complex organization of the ectexine is established through processes of self-assembly operating in a highly organized glycocalyx. Purely physical processes, including self-assembly and tensegrity, which are not under direct genetic control, play an important role. We demonstrate the complex suite of mechanisms and interactions underlying sporoderm development. It is clear that in pollen wall development, biological processes and purely physical factors are inseparably entwined." @default.
- W2313385488 created "2016-06-24" @default.
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- W2313385488 date "2016-05-01" @default.
- W2313385488 modified "2023-09-24" @default.
- W2313385488 title "Pollen Wall Substructure and Development in<i>Tanacetum vulgare</i>(Compositae: Anthemideae): Revisiting Hypotheses on Pattern Formation in Complex Cell Walls" @default.
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- W2313385488 doi "https://doi.org/10.1086/684946" @default.
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