FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2313474489> ?p ?o ?g. }

• W2313474489 endingPage "517" @default.
• W2313474489 startingPage "505" @default.
• W2313474489 abstract "Directed differentiation of human induced pluripotent stem cells (iPSCs) into retinal pigmented epithelium (RPE) holds great promise in cell replacement therapy for patients suffering from degenerative eye diseases, including age-related macular degeneration (AMD). In this study, we generated iPSCs from human dermal fibroblasts (HDFs) by electroporation with episomal plasmid vectors encoding OCT4, SOX2, KLF4, L-MYC together with p53 suppression. Intriguingly, cell reprogramming resulted in a metastable transcriptional activation and selective demethylation of neural and retinal specification-associated genes, such as OTX2, RX1 and SIX3. In contrast, RPE progenitor genes were transcriptionally silent in HDFs and descendant iPSCs. Overexpression of OCT4 and SOX2 directly stimulated the expression of OTX2, RX1 and SIX3 in HDFs and iPSCs. Luciferase and chromatin immunoprecipitation (ChIP) assays further identified an OCT4- and two SOX2-binding sites located in the proximal promoter of OTX2. Histone acetylation and methylation on the local promoter also participated in the reactivation of OTX2. The transcriptional conversion of RX1 and SIX3 genes partially attributed to DNA demethylation. Subsequently, iPSCs were induced into the RPE cells displaying the characteristics of polygonal shapes and pigments, and expressing typical RPE cell markers. Taken together, our results establish readily efficient and safe protocols to produce iPSCs and iPSC-derived RPE cells, and underline that the reactivation of anterior neural transcription factor OTX2, eye field transcription factor RX1 and SIX3 in iPSCs is a feature of pluripotency acquisition and predetermines the potential of RPE differentiation." @default.
• W2313474489 created "2016-06-24" @default.
• W2313474489 creator A5002940056 @default.
• W2313474489 creator A5007699127 @default.
• W2313474489 creator A5008134007 @default.
• W2313474489 creator A5013157565 @default.
• W2313474489 creator A5015703932 @default.
• W2313474489 creator A5019544133 @default.
• W2313474489 creator A5041294713 @default.
• W2313474489 creator A5044890663 @default.
• W2313474489 creator A5071458554 @default.
• W2313474489 creator A5079096032 @default.
• W2313474489 creator A5081316996 @default.
• W2313474489 creator A5081505822 @default.
• W2313474489 creator A5086326013 @default.
• W2313474489 creator A5090650292 @default.
• W2313474489 date "2016-01-01" @default.
• W2313474489 modified "2023-10-14" @default.
• W2313474489 title "Transcriptional Reactivation of <i>OTX2, RX1 </i>and <i>SIX3</i> during Reprogramming Contributes to the Generation of RPE Cells from Human iPSCs" @default.
• W2313474489 cites W1983847076 @default.
• W2313474489 cites W1995480864 @default.
• W2313474489 cites W2003239720 @default.
• W2313474489 cites W2008365696 @default.
• W2313474489 cites W2010387137 @default.
• W2313474489 cites W2011587839 @default.
• W2313474489 cites W2019064897 @default.
• W2313474489 cites W2029353869 @default.
• W2313474489 cites W2030291420 @default.
• W2313474489 cites W2030809398 @default.
• W2313474489 cites W2034054538 @default.
• W2313474489 cites W2042409505 @default.
• W2313474489 cites W2049176786 @default.
• W2313474489 cites W2056026334 @default.
• W2313474489 cites W2070218981 @default.
• W2313474489 cites W2070405986 @default.
• W2313474489 cites W2073508490 @default.
• W2313474489 cites W2076486071 @default.
• W2313474489 cites W2083775070 @default.
• W2313474489 cites W2092167465 @default.
• W2313474489 cites W2094866815 @default.
• W2313474489 cites W2107802984 @default.
• W2313474489 cites W2113676327 @default.
• W2313474489 cites W2124789204 @default.
• W2313474489 cites W2125987139 @default.
• W2313474489 cites W2126780067 @default.
• W2313474489 cites W2136541538 @default.
• W2313474489 cites W2141933932 @default.
• W2313474489 cites W2149968101 @default.
• W2313474489 cites W2412892414 @default.
• W2313474489 cites W2993133819 @default.
• W2313474489 doi "https://doi.org/10.7150/ijbs.14212" @default.
• W2313474489 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4807412" @default.
• W2313474489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27019633" @default.
• W2313474489 hasPublicationYear "2016" @default.
• W2313474489 type Work @default.
• W2313474489 sameAs 2313474489 @default.
• W2313474489 citedByCount "12" @default.
• W2313474489 countsByYear W23134744892016 @default.
• W2313474489 countsByYear W23134744892017 @default.
• W2313474489 countsByYear W23134744892018 @default.
• W2313474489 countsByYear W23134744892019 @default.
• W2313474489 countsByYear W23134744892020 @default.
• W2313474489 countsByYear W23134744892021 @default.
• W2313474489 countsByYear W23134744892022 @default.
• W2313474489 crossrefType "journal-article" @default.
• W2313474489 hasAuthorship W2313474489A5002940056 @default.
• W2313474489 hasAuthorship W2313474489A5007699127 @default.
• W2313474489 hasAuthorship W2313474489A5008134007 @default.
• W2313474489 hasAuthorship W2313474489A5013157565 @default.
• W2313474489 hasAuthorship W2313474489A5015703932 @default.
• W2313474489 hasAuthorship W2313474489A5019544133 @default.
• W2313474489 hasAuthorship W2313474489A5041294713 @default.
• W2313474489 hasAuthorship W2313474489A5044890663 @default.
• W2313474489 hasAuthorship W2313474489A5071458554 @default.
• W2313474489 hasAuthorship W2313474489A5079096032 @default.
• W2313474489 hasAuthorship W2313474489A5081316996 @default.
• W2313474489 hasAuthorship W2313474489A5081505822 @default.
• W2313474489 hasAuthorship W2313474489A5086326013 @default.
• W2313474489 hasAuthorship W2313474489A5090650292 @default.
• W2313474489 hasBestOaLocation W23134744891 @default.
• W2313474489 hasConcept C101762097 @default.
• W2313474489 hasConcept C104317684 @default.
• W2313474489 hasConcept C107459253 @default.
• W2313474489 hasConcept C134320426 @default.
• W2313474489 hasConcept C145103041 @default.
• W2313474489 hasConcept C1491633281 @default.
• W2313474489 hasConcept C150194340 @default.
• W2313474489 hasConcept C153911025 @default.
• W2313474489 hasConcept C16824249 @default.
• W2313474489 hasConcept C190727270 @default.
• W2313474489 hasConcept C2777933648 @default.
• W2313474489 hasConcept C2778265714 @default.
• W2313474489 hasConcept C54355233 @default.
• W2313474489 hasConcept C77255625 @default.
• W2313474489 hasConcept C86339819 @default.
• W2313474489 hasConcept C86803240 @default.
• W2313474489 hasConcept C95444343 @default.
• W2313474489 hasConceptScore W2313474489C101762097 @default.

• next