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- W2313591229 abstract "Osteoclasts are multinucleate giant cells playing key roles in bone resorption. These cells solubilize mineralized bone matrix by means of acid and protease action ; however, the precise mechanism of this process is not well known. Recently, we succeeded in the isolation of pure osteoclasts from rabbit bones and constructed a cDNA library. Using a differential screening procedure, two genes expressed predominantly in osteoclasts compared with spleen cells were isolated. One of them, OC-2, was found to encode a possible cysteine proteinase structurally related to cathepsins L and S.Moreover, by use of a rabbit OC-2 fragment as a probe, its human counterpart was cloned from a cDNA library of osteoarthritic hip bone. The cloned human cDNA (hOC-2) encoded a protein of 329 amino acid residues and its deduced amino acid sequence showed 94% homology to rabbit cathepsin K. Multiple alignment of amino acid sequences of human cathepsins B, H, L, S and K showed the highest homology of cathepsin K to cathepsin S 48%. Northern blot analysis showed that cathepsin K mRNA is expressed at high levels in some osteoarthritic hip bone and at a very high level in osteoclastoma compared to very low levels in other tissues. These results suggest that cathepsin K is closely involved in human osteoclastic bone resorption." @default.
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- W2313591229 date "1997-01-01" @default.
- W2313591229 modified "2023-10-18" @default.
- W2313591229 title "Isolation Procedure of Osteoclasts and Isolation and Characterization of Genes Predominantly Expressed in Osteoclasts." @default.
- W2313591229 doi "https://doi.org/10.1538/expanim1992.13.58" @default.
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