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- W2313602511 abstract "We report the synthesis of active polymers of superfolder green fluorescent protein (sfGFP) in one step using Click chemistry. Up to six copies of the non-natural amino acids (nnAAs) p-azido-l-phenylalanine (pAzF) or p-propargyloxy-l-phenylalanine (pPaF) were site-specifically inserted into sfGFP by cell-free protein synthesis (CFPS). sfGFP containing two or three copies of these nnAAs were coupled by copper-catalyzed azide–alkyne cycloaddition to synthesize linear or branched protein polymers, respectively. The protein polymers retained ≥63% of their specific activity (i.e., fluorescence) after coupling. Polymerization of a concentrated solution of triply substituted sfGFP resulted in fluorescent macromolecular particles. Our method can be generalized to synthesize polymers of a protein or copolymers of any two or more proteins, and the conjugation sites can be determined exactly by standard genetic manipulation. Polymers of proteins and small molecules can also be created with this technology to make a new class of scaffolds or biomaterials." @default.
- W2313602511 created "2016-06-24" @default.
- W2313602511 creator A5034438864 @default.
- W2313602511 creator A5066541730 @default.
- W2313602511 date "2013-11-21" @default.
- W2313602511 modified "2023-09-23" @default.
- W2313602511 title "Direct Polymerization of Proteins" @default.
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- W2313602511 doi "https://doi.org/10.1021/sb400116x" @default.
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