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• W2313674269 endingPage "e0152690" @default.
• W2313674269 startingPage "e0152690" @default.
• W2313674269 abstract "Although cryopreservation has been developed and optimized over the past decades, it causes various stresses, including cold shock, osmotic stress, and ice crystal formation, thereby reducing fertility. During cryopreservation, addition of cryoprotective agent (CPA) is crucial for protecting spermatozoa from freezing damage. However, the intrinsic toxicity and osmotic stress induced by CPA cause damage to spermatozoa. To identify the effects of CPA addition during cryopreservation, we assessed the motility (%), motion kinematics, capacitation status, and viability of epididymal spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining. Moreover, the effects of CPA addition were also demonstrated at the proteome level using two-dimensional electrophoresis. Our results demonstrated that CPA addition significantly reduced sperm motility (%), curvilinear velocity, viability (%), and non-capacitated spermatozoa, whereas straightness and acrosome-reacted spermatozoa increased significantly (p < 0.05). Ten proteins were differentially expressed (two decreased and eight increased) (>3 fold, p < 0.05) after CPA, whereas NADH dehydrogenase flavoprotein 2, f-actin-capping protein subunit beta, superoxide dismutase 2, and outer dense fiber protein 2 were associated with several important signaling pathways (p < 0.05). The present study provides a mechanistic basis for specific cryostresses and potential markers of CPA-induced stress. Therefore, these might provide information about the development of safe biomaterials for cryopreservation and basic ground for sperm cryopreservation." @default.
• W2313674269 created "2016-06-24" @default.
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• W2313674269 date "2016-03-31" @default.
• W2313674269 modified "2023-09-30" @default.
• W2313674269 title "Addition of Cryoprotectant Significantly Alters the Epididymal Sperm Proteome" @default.
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• W2313674269 doi "https://doi.org/10.1371/journal.pone.0152690" @default.
• W2313674269 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4816509" @default.
• W2313674269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27031703" @default.
• W2313674269 hasPublicationYear "2016" @default.
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