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- W2313752794 abstract "The sensitivity of the frog's heart to changes in the calcium content of a nutrient fluid has long been known1, 2. Utilization of this property for the estimation of calcium ions in biological fluids has, however, not been generally considered feasible, because considerable doubt has existed3 as to whether the known sensitivity is to calcium ions or to total calcium. The method here presented is based upon (1) the demonstration, simultaneously reported, that the reaction of the frog's heart is specific for calcium ions, bound calcium being without effect, and (2) elimination or control of other factors which may influence the behavior of the heart.The method consists essentially of recording the amplitude of the contraction of the frog's heart, suspended from a modified Straub cannula inserted through the aorta into the ventricle. After taking certain precautions to standardize the procedure, the concentration of calcium ions in the unknown solution or biological fluid is taken to be that of the known sol..." @default.
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- W2313752794 date "1933-06-01" @default.
- W2313752794 modified "2023-09-27" @default.
- W2313752794 title "Concentration of Calcium Ions in Biological Fluids" @default.
- W2313752794 doi "https://doi.org/10.3181/00379727-30-6924" @default.
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