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• W2313872023 endingPage "3536" @default.
• W2313872023 startingPage "3529" @default.
• W2313872023 abstract "4'-C-[(4-Trifluoromethyl-1H-1,2,3-triazol-1-yl)methyl]thymidine was synthesized and incorporated as a phosphoramidite into oligonucleotide sequences. Its applicability as a sensor for the (19)F NMR spectroscopic detection of DNA and RNA secondary structures was demonstrated. On DNA, the (19)F NMR measurements were focused on monitoring of duplex-triplex conversion, for which this fluorine-labeled 2'-deoxynucleoside proved to be a powerful sensor. This sensor seemingly favors DNA, but its behavior in the RNA environment also turned out to be informative. As a demonstration, invasion of a 2'-O-methyl oligoribonucleotide into an RNA hairpin model (HIV-1 TAR) was monitored by (19)F NMR spectroscopy. According to the thermal denaturation studies by UV spectroscopy, the effect of the 4'-C-(4-trifluoromethyl-1H-1,2,3-triazol-1-yl)methyl moiety on the stability of these DNA and RNA models was marginal." @default.
• W2313872023 created "2016-06-24" @default.
• W2313872023 creator A5035779148 @default.
• W2313872023 creator A5050029720 @default.
• W2313872023 date "2014-04-10" @default.
• W2313872023 modified "2023-10-18" @default.
• W2313872023 title "4′-<i>C</i>-[(4-Trifluoromethyl-1<i>H</i>-1,2,3-triazol-1-yl)methyl]thymidine as a Sensitive ¹⁹F NMR Sensor for the Detection of Oligonucleotide Secondary Structures" @default.
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• W2313872023 doi "https://doi.org/10.1021/jo500326j" @default.
• W2313872023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24678774" @default.
• W2313872023 hasPublicationYear "2014" @default.
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