Matches in SemOpenAlex for { <https://semopenalex.org/work/W2313895977> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2313895977 endingPage "36" @default.
- W2313895977 startingPage "29" @default.
- W2313895977 abstract "Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) can provide information on the spatial distribution and amount of numerous molecules on a single tissue section. A single sample that can be used in both positive and negative ion analyses is needed, since this can reduce losses of rare clinical samples. Although p-nitroaniline (NIT) has been shown to be applicable for use as a matrix in MALDI-IMS in the positive and negative ion modes, it is volatile and instable under vacuum conditions. In this study, we applied NIT as a volatile matrix for atmospheric pressure (AP) MALDI-IMS analysis, using a Mass Microscope, because NIT is considered to remain stable on the sample under AP conditions. Our objective was to optimize NIT usage by evaluating peak intensities acquired in the positive and negative ion modes. We evaluated signal intensities under 3 different conditions; dilution at different volumes, at different concentrations, and in two types of solvents. We also measured the matrix weight deposited on samples and determined the optimum condition with respect to matrix weight and signal intensity. The optimum condition was determined as follows; a solvent volume of 2 mL, an NIT concentration of 30 mg/mL, and 100% methanol as the solvent. Mass spectra obtained using these conditions provided comparable signals with 2,5-dihydroxybenzoic acid and 9-aminoacridine. Using tandem mass spectrometry, the most intense signals in both ion modes were identified as phosphatidylcholine and phosphatidylinositol. Moreover, the lipid distribution in the mouse cerebellum could be visualized at a high spatial resolution of 5 μm on a single section, in both the positive and negative ion modes. This study shows that optimization of NIT is determined by 3 parameters (solvent volume, NIT concentration, type of solvent). Using the optimum condition, it was possible to analyze the same tissue section by AP MALDI-IMS in both the positive and negative ion modes, at a sufficiently high sensitivity for tandem mass spectrometry and imaging at a spatial resolution of 5 μm." @default.
- W2313895977 created "2016-06-24" @default.
- W2313895977 creator A5007312632 @default.
- W2313895977 creator A5021806063 @default.
- W2313895977 creator A5057392413 @default.
- W2313895977 creator A5076905995 @default.
- W2313895977 creator A5078373195 @default.
- W2313895977 date "2014-01-01" @default.
- W2313895977 modified "2023-09-27" @default.
- W2313895977 title "Atmospheric Pressure MALDI-IMS Using p-Nitroaniline as the Matrix at High Spatial Resolution in the Positive and Negative Ion Modes" @default.
- W2313895977 cites W1971626294 @default.
- W2313895977 cites W1982555544 @default.
- W2313895977 cites W1986581829 @default.
- W2313895977 cites W2004574784 @default.
- W2313895977 cites W2019494120 @default.
- W2313895977 cites W2030024670 @default.
- W2313895977 cites W2035244024 @default.
- W2313895977 cites W2046352381 @default.
- W2313895977 cites W2054905900 @default.
- W2313895977 cites W2055319410 @default.
- W2313895977 cites W2081380820 @default.
- W2313895977 cites W2084236003 @default.
- W2313895977 cites W2086883421 @default.
- W2313895977 cites W2147502416 @default.
- W2313895977 cites W2323732179 @default.
- W2313895977 doi "https://doi.org/10.5702/massspec.14-57" @default.
- W2313895977 hasPublicationYear "2014" @default.
- W2313895977 type Work @default.
- W2313895977 sameAs 2313895977 @default.
- W2313895977 citedByCount "2" @default.
- W2313895977 countsByYear W23138959772016 @default.
- W2313895977 countsByYear W23138959772017 @default.
- W2313895977 crossrefType "journal-article" @default.
- W2313895977 hasAuthorship W2313895977A5007312632 @default.
- W2313895977 hasAuthorship W2313895977A5021806063 @default.
- W2313895977 hasAuthorship W2313895977A5057392413 @default.
- W2313895977 hasAuthorship W2313895977A5076905995 @default.
- W2313895977 hasAuthorship W2313895977A5078373195 @default.
- W2313895977 hasBestOaLocation W23138959771 @default.
- W2313895977 hasConcept C101092202 @default.
- W2313895977 hasConcept C106487976 @default.
- W2313895977 hasConcept C111368507 @default.
- W2313895977 hasConcept C113196181 @default.
- W2313895977 hasConcept C127313418 @default.
- W2313895977 hasConcept C138268822 @default.
- W2313895977 hasConcept C145148216 @default.
- W2313895977 hasConcept C150394285 @default.
- W2313895977 hasConcept C154945302 @default.
- W2313895977 hasConcept C162356407 @default.
- W2313895977 hasConcept C162711632 @default.
- W2313895977 hasConcept C178790620 @default.
- W2313895977 hasConcept C185592680 @default.
- W2313895977 hasConcept C200239111 @default.
- W2313895977 hasConcept C2780471494 @default.
- W2313895977 hasConcept C41008148 @default.
- W2313895977 hasConcept C43617362 @default.
- W2313895977 hasConcept C75280812 @default.
- W2313895977 hasConceptScore W2313895977C101092202 @default.
- W2313895977 hasConceptScore W2313895977C106487976 @default.
- W2313895977 hasConceptScore W2313895977C111368507 @default.
- W2313895977 hasConceptScore W2313895977C113196181 @default.
- W2313895977 hasConceptScore W2313895977C127313418 @default.
- W2313895977 hasConceptScore W2313895977C138268822 @default.
- W2313895977 hasConceptScore W2313895977C145148216 @default.
- W2313895977 hasConceptScore W2313895977C150394285 @default.
- W2313895977 hasConceptScore W2313895977C154945302 @default.
- W2313895977 hasConceptScore W2313895977C162356407 @default.
- W2313895977 hasConceptScore W2313895977C162711632 @default.
- W2313895977 hasConceptScore W2313895977C178790620 @default.
- W2313895977 hasConceptScore W2313895977C185592680 @default.
- W2313895977 hasConceptScore W2313895977C200239111 @default.
- W2313895977 hasConceptScore W2313895977C2780471494 @default.
- W2313895977 hasConceptScore W2313895977C41008148 @default.
- W2313895977 hasConceptScore W2313895977C43617362 @default.
- W2313895977 hasConceptScore W2313895977C75280812 @default.
- W2313895977 hasIssue "4" @default.
- W2313895977 hasLocation W23138959771 @default.
- W2313895977 hasOpenAccess W2313895977 @default.
- W2313895977 hasPrimaryLocation W23138959771 @default.
- W2313895977 hasRelatedWork W1988241777 @default.
- W2313895977 hasRelatedWork W2019147148 @default.
- W2313895977 hasRelatedWork W2019408827 @default.
- W2313895977 hasRelatedWork W2026642978 @default.
- W2313895977 hasRelatedWork W2048919949 @default.
- W2313895977 hasRelatedWork W2053777062 @default.
- W2313895977 hasRelatedWork W2069636393 @default.
- W2313895977 hasRelatedWork W2145852303 @default.
- W2313895977 hasRelatedWork W2279204696 @default.
- W2313895977 hasRelatedWork W2320691067 @default.
- W2313895977 hasVolume "62" @default.
- W2313895977 isParatext "false" @default.
- W2313895977 isRetracted "false" @default.
- W2313895977 magId "2313895977" @default.
- W2313895977 workType "article" @default.