Matches in SemOpenAlex for { <https://semopenalex.org/work/W2313895979> ?p ?o ?g. }
- W2313895979 endingPage "3370" @default.
- W2313895979 startingPage "3361" @default.
- W2313895979 abstract "Proteolytic enzymes in the gut represent one of the biggest barriers against oral delivery of therapeutic proteins and peptides. In the current study, we explored the effect of poly(ethylene glycol) 400 (PEG 400), a commonly used crowding agent, on insulin degradation mediated by α-chymotrypsin (α-CT). Without PEG 400, insulin was quickly cleaved by α-CT to generate inactive degradation products. In comparison, incorporation of PEG 400 resulted in reaction mixtures with retained biological activity. The analysis on the conformation of α-CT and the local environment of the enzyme's active site unraveled that PEG 400 altered the conformation of α-CT to prevent the inactivation of insulin via stabilization of active intermediates. These findings indicated that PEG 400 may provide a promising addition toward oral delivery of insulin." @default.
- W2313895979 created "2016-06-24" @default.
- W2313895979 creator A5013432757 @default.
- W2313895979 creator A5015059358 @default.
- W2313895979 creator A5026171887 @default.
- W2313895979 creator A5047781906 @default.
- W2313895979 creator A5066716873 @default.
- W2313895979 creator A5066933998 @default.
- W2313895979 creator A5085711183 @default.
- W2313895979 date "2014-04-21" @default.
- W2313895979 modified "2023-09-24" @default.
- W2313895979 title "Poly(ethylene glycol)-Mediated Conformational Alteration of α-Chymotrypsin Prevents Inactivation of Insulin by Stabilizing Active Intermediates" @default.
- W2313895979 cites W1520238517 @default.
- W2313895979 cites W1845838221 @default.
- W2313895979 cites W187584755 @default.
- W2313895979 cites W1970898345 @default.
- W2313895979 cites W1986477299 @default.
- W2313895979 cites W1986559446 @default.
- W2313895979 cites W1986689667 @default.
- W2313895979 cites W1998122941 @default.
- W2313895979 cites W2002816235 @default.
- W2313895979 cites W2002831204 @default.
- W2313895979 cites W2004671393 @default.
- W2313895979 cites W2014886122 @default.
- W2313895979 cites W2015769453 @default.
- W2313895979 cites W2018699677 @default.
- W2313895979 cites W2019828089 @default.
- W2313895979 cites W2020374578 @default.
- W2313895979 cites W2020659601 @default.
- W2313895979 cites W2022347555 @default.
- W2313895979 cites W2022416858 @default.
- W2313895979 cites W2036000659 @default.
- W2313895979 cites W2040243871 @default.
- W2313895979 cites W2043903692 @default.
- W2313895979 cites W2046152615 @default.
- W2313895979 cites W2054570958 @default.
- W2313895979 cites W2064568512 @default.
- W2313895979 cites W2066962978 @default.
- W2313895979 cites W2068712458 @default.
- W2313895979 cites W2069632973 @default.
- W2313895979 cites W2071569006 @default.
- W2313895979 cites W2076069488 @default.
- W2313895979 cites W2093835533 @default.
- W2313895979 cites W2094701081 @default.
- W2313895979 cites W2112443699 @default.
- W2313895979 cites W2123398255 @default.
- W2313895979 cites W2147700328 @default.
- W2313895979 cites W2158671989 @default.
- W2313895979 cites W2160891110 @default.
- W2313895979 cites W2162139246 @default.
- W2313895979 cites W291763739 @default.
- W2313895979 cites W4312417761 @default.
- W2313895979 doi "https://doi.org/10.1021/mp500001n" @default.
- W2313895979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24720816" @default.
- W2313895979 hasPublicationYear "2014" @default.
- W2313895979 type Work @default.
- W2313895979 sameAs 2313895979 @default.
- W2313895979 citedByCount "2" @default.
- W2313895979 countsByYear W23138959792014 @default.
- W2313895979 countsByYear W23138959792015 @default.
- W2313895979 crossrefType "journal-article" @default.
- W2313895979 hasAuthorship W2313895979A5013432757 @default.
- W2313895979 hasAuthorship W2313895979A5015059358 @default.
- W2313895979 hasAuthorship W2313895979A5026171887 @default.
- W2313895979 hasAuthorship W2313895979A5047781906 @default.
- W2313895979 hasAuthorship W2313895979A5066716873 @default.
- W2313895979 hasAuthorship W2313895979A5066933998 @default.
- W2313895979 hasAuthorship W2313895979A5085711183 @default.
- W2313895979 hasConcept C10138342 @default.
- W2313895979 hasConcept C116073593 @default.
- W2313895979 hasConcept C12554922 @default.
- W2313895979 hasConcept C126322002 @default.
- W2313895979 hasConcept C162324750 @default.
- W2313895979 hasConcept C178790620 @default.
- W2313895979 hasConcept C181199279 @default.
- W2313895979 hasConcept C185592680 @default.
- W2313895979 hasConcept C202751555 @default.
- W2313895979 hasConcept C2776964284 @default.
- W2313895979 hasConcept C2777516009 @default.
- W2313895979 hasConcept C2778401398 @default.
- W2313895979 hasConcept C2779149719 @default.
- W2313895979 hasConcept C2779306644 @default.
- W2313895979 hasConcept C2780340462 @default.
- W2313895979 hasConcept C41183919 @default.
- W2313895979 hasConcept C54400483 @default.
- W2313895979 hasConcept C55493867 @default.
- W2313895979 hasConcept C71924100 @default.
- W2313895979 hasConcept C86803240 @default.
- W2313895979 hasConceptScore W2313895979C10138342 @default.
- W2313895979 hasConceptScore W2313895979C116073593 @default.
- W2313895979 hasConceptScore W2313895979C12554922 @default.
- W2313895979 hasConceptScore W2313895979C126322002 @default.
- W2313895979 hasConceptScore W2313895979C162324750 @default.
- W2313895979 hasConceptScore W2313895979C178790620 @default.
- W2313895979 hasConceptScore W2313895979C181199279 @default.
- W2313895979 hasConceptScore W2313895979C185592680 @default.
- W2313895979 hasConceptScore W2313895979C202751555 @default.
- W2313895979 hasConceptScore W2313895979C2776964284 @default.