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- W2314013531 abstract "The ability to visualize the distributions of specific proteins with a light microscope and fluorescent probes is largely responsible for our current understanding of cellular structure. A major limitation of this approach arises from the blurring effects of diffraction, which decreases resolution and limits the ability to obtain information at the nanoscale. There has been a tremendous drive to develop optical and computational methods that improve the resolution of the light microscope, and structured illumination microscopy (SIM) is one solution. This method uses patterned illumination to double both lateral and axial resolution. Nikon's N-SIM is a commercial system that integrates the most desirable features of light microscopy, specific labeling of molecules, and live cell imaging, with structured illumination. This provides the ability to achieve super resolution suitable for a range of biological applications." @default.
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- W2314013531 date "2012-07-01" @default.
- W2314013531 modified "2023-10-18" @default.
- W2314013531 title "Live Cell Super-Resolution Imaging with N-SIM" @default.
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- W2314013531 doi "https://doi.org/10.1017/s1551929512000375" @default.
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