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- W2314440282 abstract "Summary Theoretical and experimental bases of TILLING and FOX-hunting, new tools for precise identification of genes in functional studies are reviewed. TILLING (Targeting Induced Local Lesions IN Genomes) is a technique combining chemical mutagenesis with its sensitive and precise detection. The method involves PCR amplification of DNA samples pooled following extraction from a collection of chemically-treated organisms and a subsequent screening for mutations using Cel1 endonuclease, detecting mismatches in heteroduplexes [52]. FOX-hunting (Full-length cDNA Over-eXpressing gene hunting system) is a new method of plant gene overexpression, which enables a quick gene isolation and sequencing, paralelly with functional studies." @default.
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- W2314440282 date "2011-02-01" @default.
- W2314440282 modified "2023-09-26" @default.
- W2314440282 title "Tilling and Fox-Hunting: New Methods for Functional Analysis of Genes" @default.
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- W2314440282 doi "https://doi.org/10.2478/v10052-011-0001-6" @default.
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