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- W2314463318 abstract "Post-transcriptional RNA modifications that are introduced during the multistep ribosome biogenesis process are essential for protein synthesis. The current lack of a comprehensive method for a fast quantitative analysis of rRNA modifications significantly limits our understanding of how individual modification steps are coordinated during biogenesis inside the cell. Here, an LC-MS approach has been developed and successfully applied for quantitative monitoring of 29 out of 36 modified residues in the 16S and 23S rRNA from Escherichia coli. An isotope labeling strategy is described for efficient identification of ribose and base methylations, and a novel metabolic labeling approach is presented to allow identification of MS-silent pseudouridine modifications. The method was used to measure relative abundances of modified residues in incomplete ribosomal subunits compared to a mature 15N-labeled rRNA standard, and a number of modifications in both 16S and 23S rRNA were present in substoichiometric amounts in the preribosomal particles. The RNA modification levels correlate well with previously obtained profiles for the ribosomal proteins, suggesting that RNA is modified in a schedule comparable to the association of the ribosomal proteins. Importantly, this study establishes an efficient workflow for a global monitoring of ribosomal modifications that will contribute to a better understanding of mechanisms of RNA modifications and their impact on intracellular processes in the future." @default.
- W2314463318 created "2016-06-24" @default.
- W2314463318 creator A5028883283 @default.
- W2314463318 creator A5074143608 @default.
- W2314463318 date "2014-01-27" @default.
- W2314463318 modified "2023-10-17" @default.
- W2314463318 title "Quantitative Analysis of rRNA Modifications Using Stable Isotope Labeling and Mass Spectrometry" @default.
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- W2314463318 doi "https://doi.org/10.1021/ja412084b" @default.
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