FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2314502012> ?p ?o ?g. }

• W2314502012 endingPage "195" @default.
• W2314502012 startingPage "185" @default.
• W2314502012 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 97:185-195 (2012) - DOI: https://doi.org/10.3354/dao02423 Evaluation of a filtration-based method for ­detecting Batrachochytrium dendrobatidis in ­natural bodies of water Oliver J. Hyman*, James P. Collins Arizona State University, School of Life Sciences, 427 E. Tyler Mall, LSC 410, Tempe, Arizona 85287, USA *Email: ohyman@asu.edu ABSTRACT: Infectious diseases are emerging as a significant threat to wildlife. The resulting increased effort to monitor wildlife diseases is driving the development of innovative pathogen monitoring techniques, including many polymerase chain reaction (PCR)-based diagnostics. Despite the utility of these PCR-based techniques, there is still much to be learned about their ability to accurately detect target pathogens in nature. We assessed the diagnostic sensitivity of a PCR-based water filtration technique to detect the directly transmitted aquatic fungal pathogen Batrachochytrium dendrobatidis (Bd) by comparing the results of 4 repeated filter sampling events from 20 ponds to those of skin swabs from ca. 60 boreal chorus frogs Pseudacris maculata from each pond. Filters failed to detect Bd in 31 to 77% of the swab-positive ponds, depending on the time of sampling. However, after 3 repeated sampling events, filtration of small volumes of water (ca. 600 ml) correctly identified 94% of the ponds that tested Bd positive with swabbing, with the highest rates of detection occurring after breeding but before larvae reached metamorphosis. Our results are a case study demonstrating the importance of timing and resampling for the detection of an aquatic microbial pathogen, Bd, from water. This will be a useful technique for monitoring Bd, but additional data are needed to test the degree to which our findings are species or population specific. Future studies need to examine the sensitivity of this technique in other habitats and species that host Bd. These studies will aid in the development of cost-effective monitoring regimes for Bd and potentially other aquatic pathogens. KEY WORDS: Amphibian chytrid · Environmental monitoring · Detection · Pathogen · ­Chytridiomycosis · qPCR · Pseudacris Full text in pdf format PreviousNextCite this article as: Hyman OJ, Collins JP (2012) Evaluation of a filtration-based method for ­detecting Batrachochytrium dendrobatidis in ­natural bodies of water. Dis Aquat Org 97:185-195. https://doi.org/10.3354/dao02423 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 97, No. 3. Online publication date: January 24, 2012 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2012 Inter-Research." @default.
• W2314502012 created "2016-06-24" @default.
• W2314502012 creator A5018784376 @default.
• W2314502012 creator A5024966207 @default.
• W2314502012 date "2012-01-24" @default.
• W2314502012 modified "2023-10-18" @default.
• W2314502012 title "Evaluation of a filtration-based method for detecting Batrachochytrium dendrobatidis in natural bodies of water" @default.
• W2314502012 cites W1979009666 @default.
• W2314502012 cites W1991750327 @default.
• W2314502012 cites W1996357537 @default.
• W2314502012 cites W2006712611 @default.
• W2314502012 cites W2016008920 @default.
• W2314502012 cites W2019290739 @default.
• W2314502012 cites W2019364491 @default.
• W2314502012 cites W2026764344 @default.
• W2314502012 cites W2032509292 @default.
• W2314502012 cites W2042977092 @default.
• W2314502012 cites W2057589068 @default.
• W2314502012 cites W2062370967 @default.
• W2314502012 cites W2066257994 @default.
• W2314502012 cites W2066935620 @default.
• W2314502012 cites W2066953299 @default.
• W2314502012 cites W2076873445 @default.
• W2314502012 cites W2081857169 @default.
• W2314502012 cites W2102956415 @default.
• W2314502012 cites W2121354575 @default.
• W2314502012 cites W2122607321 @default.
• W2314502012 cites W2136477173 @default.
• W2314502012 cites W2138291917 @default.
• W2314502012 cites W2139060243 @default.
• W2314502012 cites W2150891147 @default.
• W2314502012 cites W2162530050 @default.
• W2314502012 cites W2170333398 @default.
• W2314502012 cites W2324404733 @default.
• W2314502012 cites W4252323872 @default.
• W2314502012 doi "https://doi.org/10.3354/dao02423" @default.
• W2314502012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22422089" @default.
• W2314502012 hasPublicationYear "2012" @default.
• W2314502012 type Work @default.
• W2314502012 sameAs 2314502012 @default.
• W2314502012 citedByCount "42" @default.
• W2314502012 countsByYear W23145020122012 @default.
• W2314502012 countsByYear W23145020122013 @default.
• W2314502012 countsByYear W23145020122014 @default.
• W2314502012 countsByYear W23145020122015 @default.
• W2314502012 countsByYear W23145020122016 @default.
• W2314502012 countsByYear W23145020122017 @default.
• W2314502012 countsByYear W23145020122018 @default.
• W2314502012 countsByYear W23145020122019 @default.
• W2314502012 countsByYear W23145020122020 @default.
• W2314502012 countsByYear W23145020122021 @default.
• W2314502012 countsByYear W23145020122022 @default.
• W2314502012 countsByYear W23145020122023 @default.
• W2314502012 crossrefType "journal-article" @default.
• W2314502012 hasAuthorship W2314502012A5018784376 @default.
• W2314502012 hasAuthorship W2314502012A5024966207 @default.
• W2314502012 hasBestOaLocation W23145020121 @default.
• W2314502012 hasConcept C106131492 @default.
• W2314502012 hasConcept C140779682 @default.
• W2314502012 hasConcept C18903297 @default.
• W2314502012 hasConcept C2776460866 @default.
• W2314502012 hasConcept C2781273459 @default.
• W2314502012 hasConcept C29376679 @default.
• W2314502012 hasConcept C31972630 @default.
• W2314502012 hasConcept C41008148 @default.
• W2314502012 hasConcept C42972112 @default.
• W2314502012 hasConcept C71924100 @default.
• W2314502012 hasConcept C86803240 @default.
• W2314502012 hasConcept C89423630 @default.
• W2314502012 hasConcept C90856448 @default.
• W2314502012 hasConceptScore W2314502012C106131492 @default.
• W2314502012 hasConceptScore W2314502012C140779682 @default.
• W2314502012 hasConceptScore W2314502012C18903297 @default.
• W2314502012 hasConceptScore W2314502012C2776460866 @default.
• W2314502012 hasConceptScore W2314502012C2781273459 @default.
• W2314502012 hasConceptScore W2314502012C29376679 @default.
• W2314502012 hasConceptScore W2314502012C31972630 @default.
• W2314502012 hasConceptScore W2314502012C41008148 @default.
• W2314502012 hasConceptScore W2314502012C42972112 @default.
• W2314502012 hasConceptScore W2314502012C71924100 @default.
• W2314502012 hasConceptScore W2314502012C86803240 @default.
• W2314502012 hasConceptScore W2314502012C89423630 @default.
• W2314502012 hasConceptScore W2314502012C90856448 @default.
• W2314502012 hasIssue "3" @default.
• W2314502012 hasLocation W23145020121 @default.
• W2314502012 hasLocation W23145020122 @default.
• W2314502012 hasOpenAccess W2314502012 @default.
• W2314502012 hasPrimaryLocation W23145020121 @default.
• W2314502012 hasRelatedWork W1909982228 @default.
• W2314502012 hasRelatedWork W2019126225 @default.
• W2314502012 hasRelatedWork W2078725521 @default.
• W2314502012 hasRelatedWork W2103930565 @default.
• W2314502012 hasRelatedWork W2169412618 @default.
• W2314502012 hasRelatedWork W2753069249 @default.
• W2314502012 hasRelatedWork W2784162595 @default.
• W2314502012 hasRelatedWork W2885511148 @default.
• W2314502012 hasRelatedWork W2921575269 @default.
• W2314502012 hasRelatedWork W3109411788 @default.

• next