Matches in SemOpenAlex for { <https://semopenalex.org/work/W2314552017> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2314552017 endingPage "329" @default.
- W2314552017 startingPage "327" @default.
- W2314552017 abstract "In recent years, studies of receptors for many polypeptide hormones have focused on adenylate cyclase activity and hormone binding sites in isolated cells and purified plasma membrane preparations. These studies have provided major insights regarding the physiology of hormone action. However, relatively little has been learned of the molecular properties of receptors or of the chemical mechanisms involved in hormone receptor interactions. The major obstacle to obtaining a more basic understanding of receptors is their membrane location. While some information has been obtained with solubilized hormone-sensitive adenylate cyclase preparations (1-3), receptors must ultimately be studied in their natural membranous environment. The lactoperoxidase-catalyzed iodination of exposed tyrosines has been used to label protein components of plasma membranes of several types of cells (4-6). The surface specificity of this enzyme suggested that it could provide useful information regarding the spatial arrangement of receptor components within an intact membrane. This report demonstrates the effects of iodination of highly purified plasma membranes from rat liver on adenylate cyclase activity in the membranes. Materials and Methods. Lactoperoxidase, B grade, estimated purity 60%, was obtained from Calbiochem. Carrier-free 125I was from Amersham/Searle. Acrylamide, N'N'-methylenebisacrylamide, and N,N, N, N-tetramethylethylenediamine were obtained from Eastman. Plasma membranes were prepared by a modification (7) of the procedure of Neville (8). Adenylate cyclase was assayed as described previously (7) except that [32P]cAMP was separated from [32P]ATP by the aluminum oxide method devised by Ramachandran (9). Protein was measured by the procedure of Lowry et al. (10). For amino acid analysis, liver membranes, 2 mg of protein, were hydrolyzed for 24 h at 100° in 6 N HCl. Amino acids were then estimated using a Spinco 120C amino-acid analyzer." @default.
- W2314552017 created "2016-06-24" @default.
- W2314552017 creator A5007455516 @default.
- W2314552017 date "1976-07-01" @default.
- W2314552017 modified "2023-09-25" @default.
- W2314552017 title "Iodination of Liver Plasma Membranes Using Lactoperoxidase: Effects on Adenylate Cyclase Activity" @default.
- W2314552017 doi "https://doi.org/10.3181/00379727-152-39389" @default.
- W2314552017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/181757" @default.
- W2314552017 hasPublicationYear "1976" @default.
- W2314552017 type Work @default.
- W2314552017 sameAs 2314552017 @default.
- W2314552017 citedByCount "1" @default.
- W2314552017 crossrefType "journal-article" @default.
- W2314552017 hasAuthorship W2314552017A5007455516 @default.
- W2314552017 hasConcept C121608353 @default.
- W2314552017 hasConcept C162008176 @default.
- W2314552017 hasConcept C170493617 @default.
- W2314552017 hasConcept C180526460 @default.
- W2314552017 hasConcept C181199279 @default.
- W2314552017 hasConcept C185592680 @default.
- W2314552017 hasConcept C201956729 @default.
- W2314552017 hasConcept C23589133 @default.
- W2314552017 hasConcept C2780069284 @default.
- W2314552017 hasConcept C41625074 @default.
- W2314552017 hasConcept C530470458 @default.
- W2314552017 hasConcept C54355233 @default.
- W2314552017 hasConcept C55493867 @default.
- W2314552017 hasConcept C71315377 @default.
- W2314552017 hasConcept C86803240 @default.
- W2314552017 hasConceptScore W2314552017C121608353 @default.
- W2314552017 hasConceptScore W2314552017C162008176 @default.
- W2314552017 hasConceptScore W2314552017C170493617 @default.
- W2314552017 hasConceptScore W2314552017C180526460 @default.
- W2314552017 hasConceptScore W2314552017C181199279 @default.
- W2314552017 hasConceptScore W2314552017C185592680 @default.
- W2314552017 hasConceptScore W2314552017C201956729 @default.
- W2314552017 hasConceptScore W2314552017C23589133 @default.
- W2314552017 hasConceptScore W2314552017C2780069284 @default.
- W2314552017 hasConceptScore W2314552017C41625074 @default.
- W2314552017 hasConceptScore W2314552017C530470458 @default.
- W2314552017 hasConceptScore W2314552017C54355233 @default.
- W2314552017 hasConceptScore W2314552017C55493867 @default.
- W2314552017 hasConceptScore W2314552017C71315377 @default.
- W2314552017 hasConceptScore W2314552017C86803240 @default.
- W2314552017 hasIssue "3" @default.
- W2314552017 hasLocation W23145520171 @default.
- W2314552017 hasLocation W23145520172 @default.
- W2314552017 hasOpenAccess W2314552017 @default.
- W2314552017 hasPrimaryLocation W23145520171 @default.
- W2314552017 hasRelatedWork W1445443942 @default.
- W2314552017 hasRelatedWork W1966025046 @default.
- W2314552017 hasRelatedWork W2089217617 @default.
- W2314552017 hasRelatedWork W2116920167 @default.
- W2314552017 hasRelatedWork W2314552017 @default.
- W2314552017 hasRelatedWork W2412314554 @default.
- W2314552017 hasRelatedWork W2414462586 @default.
- W2314552017 hasRelatedWork W2491304889 @default.
- W2314552017 hasRelatedWork W296922784 @default.
- W2314552017 hasRelatedWork W2220556965 @default.
- W2314552017 hasVolume "152" @default.
- W2314552017 isParatext "false" @default.
- W2314552017 isRetracted "false" @default.
- W2314552017 magId "2314552017" @default.
- W2314552017 workType "article" @default.