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- W2314838816 abstract "An analytical method has been developed to determine the amount of chlorhexidine (CH) in mouse tissues by mass fragmentography (MF). Using a combined gas chromatography/mass spectrometric method is superior because of its quick separation, mass spectrum specificity and high level of detection.It consisted of the following procedures:(1) Mouse tissues were homogenized in 0.1N HCl solutions using an ultrasonic homogenizer, and then centrifuged.(2) The CH in the resulting supernatant fractions was purified on an Extrelut column with ethyl acetate.(3) The eluted CH was converted to a triazine derivative with trifluoroacetic anhydride.(4) After dissolving in 0.2M trimethylanilinium hydroxide in methanol, the derivative was subjected to on-column methylation on a gas chromatographic column.(5) The CH triazine N-methyl derivative was identified and determined by MF.When CH was 1-10μg, its recovery from mouse tissue was more than 80%. Thus, the present method can be considered simple, rapid, sensitive, and quite specific for CH." @default.
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- W2314838816 date "1982-01-01" @default.
- W2314838816 modified "2023-10-16" @default.
- W2314838816 title "Determination by mass fragmentography of the chlorhexidine in biological samples." @default.
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- W2314838816 doi "https://doi.org/10.1265/jjh.37.762" @default.
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