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- W2314839222 abstract "Clinical investigation suggests that viral infection is involved in middle ear diseases including otitis media with effusion. We studied experimentally the direct effect of influenza A virus on the ciliary activity in the eustachian tube. Guinea pigs with normal otoscopic examination were used. Thin mucosal materials including epithelial layer of the midportion of the eustachian tube were obtained from each animal. Each material was immersed in RPMI 1640 medium. The most active ciliated cell of each culture was selected to be examined for ciliary activity (beats/min) at an ambient temperature of 30°C. Then each medium was replaced by RPMI 1640 containing a variety of influenza A viruses, and time-course changes of the ciliary activity of cells whose baseline activity was previously determined, up to 48 hours. Some materials cultured in the same fresh RPMI 1640 containing no virus served as controls. In the medium containing low concentrations of virus, ciliated cells did not demonstrate a dramatic decrease of ciliary activity. However, ciliary activity was decreased dramatically in the presence of higher concentrations (3.3×106PFU/ml), and most cells stopped their beating activity, Cultures exposed to virus were perfused with fresh RPMI 1640 for 12 hours to remove the virus, but quick recovery of the ciliary activity was not observed during observation, up to 12 hours." @default.
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- W2314839222 date "1990-01-01" @default.
- W2314839222 modified "2023-10-04" @default.
- W2314839222 title "Influence of influenza A virus on ciliary activity of cultured eustachian tube mucosa." @default.
- W2314839222 doi "https://doi.org/10.5631/jibirin.83.1243" @default.
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