FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2314904034> ?p ?o ?g. }

• W2314904034 endingPage "1240" @default.
• W2314904034 startingPage "1232" @default.
• W2314904034 abstract "Novel protocols were developed to accurately quantify reduced (GSH), oxidized (GSSG) and total (tGSH) glutathione in biological samples using molecular speciated isotope dilution mass spectrometry (SIDMS). For GSH and GSSG measurement, the sample was spiked with isotopically enriched analogues of the analytes (310GSH and 616GSSG), along with N-ethylmaleimide (NEM), and treated with acetonitrile to solubilize the endogenous analytes via protein precipitation and equilibrate them with the spikes. The supernatant was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the analytes were quantified with simultaneous tracking and correction for auto-oxidation of GSH to GSSG. For tGSH assay, a 310GSH-spiked sample was treated with dithiothreitol (DTT) to convert disulfide-bonded glutathione to GSH. After removing the protein, the supernatant was analyzed by LC-MS/MS and the analyte was quantified by single-spiking isotope dilution mass spectrometry (IDMS). The mathematical relationships in IDMS and SIDMS quantifications are based on isotopic ratios and do not involve calibration curves. The protocols were validated using spike recovery tests and by analyzing synthetic standard solutions. Red blood cell (RBC) and saliva samples obtained from healthy subjects, and whole blood samples collected and shipped from a remote location were analyzed. The concentrations of tGSH in the RBC and whole blood samples were 2 orders of magnitude higher than those found in saliva. The fractions of GSSG were 0.2–2.2% (RBC and blood) and 15–47% (saliva) of the free glutathione (GSH + 2xGSSG) in the corresponding samples. Up to 3% GSH was auto-oxidized to GSSG during sample workup; the highest oxidations (>1%) were in the saliva samples." @default.
• W2314904034 created "2016-06-24" @default.
• W2314904034 creator A5017633424 @default.
• W2314904034 creator A5032630657 @default.
• W2314904034 creator A5038378682 @default.
• W2314904034 creator A5052022866 @default.
• W2314904034 creator A5062539134 @default.
• W2314904034 creator A5068055890 @default.
• W2314904034 creator A5069065790 @default.
• W2314904034 creator A5070853499 @default.
• W2314904034 creator A5084647017 @default.
• W2314904034 date "2015-01-08" @default.
• W2314904034 modified "2023-09-23" @default.
• W2314904034 title "Molecular Speciated Isotope Dilution Mass Spectrometric Methods for Accurate, Reproducible and Direct Quantification of Reduced, Oxidized and Total Glutathione in Biological Samples" @default.
• W2314904034 cites W134833067 @default.
• W2314904034 cites W139865141 @default.
• W2314904034 cites W1970655708 @default.
• W2314904034 cites W1980199955 @default.
• W2314904034 cites W1981543241 @default.
• W2314904034 cites W1984511481 @default.
• W2314904034 cites W1985510083 @default.
• W2314904034 cites W1992377286 @default.
• W2314904034 cites W2002283108 @default.
• W2314904034 cites W2007270114 @default.
• W2314904034 cites W2015628906 @default.
• W2314904034 cites W2026846060 @default.
• W2314904034 cites W2031028849 @default.
• W2314904034 cites W2038168736 @default.
• W2314904034 cites W2041162400 @default.
• W2314904034 cites W2042363579 @default.
• W2314904034 cites W2042752945 @default.
• W2314904034 cites W2043912977 @default.
• W2314904034 cites W2048005287 @default.
• W2314904034 cites W2048563316 @default.
• W2314904034 cites W2049968430 @default.
• W2314904034 cites W2050594626 @default.
• W2314904034 cites W2055898915 @default.
• W2314904034 cites W2057521823 @default.
• W2314904034 cites W2061342459 @default.
• W2314904034 cites W2062669701 @default.
• W2314904034 cites W2064154241 @default.
• W2314904034 cites W2068922516 @default.
• W2314904034 cites W2073334129 @default.
• W2314904034 cites W2074413402 @default.
• W2314904034 cites W2075999169 @default.
• W2314904034 cites W2080270751 @default.
• W2314904034 cites W2081359173 @default.
• W2314904034 cites W2081816257 @default.
• W2314904034 cites W2084438642 @default.
• W2314904034 cites W2084605188 @default.
• W2314904034 cites W2086646140 @default.
• W2314904034 cites W2087087127 @default.
• W2314904034 cites W2093221676 @default.
• W2314904034 cites W2116567644 @default.
• W2314904034 cites W2132860985 @default.
• W2314904034 cites W2149907341 @default.
• W2314904034 cites W2150634361 @default.
• W2314904034 cites W2154586413 @default.
• W2314904034 cites W2157363358 @default.
• W2314904034 cites W2335608743 @default.
• W2314904034 cites W4245957869 @default.
• W2314904034 cites W4247645700 @default.
• W2314904034 cites W66119057 @default.
• W2314904034 doi "https://doi.org/10.1021/ac503933t" @default.
• W2314904034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25519489" @default.
• W2314904034 hasPublicationYear "2015" @default.
• W2314904034 type Work @default.
• W2314904034 sameAs 2314904034 @default.
• W2314904034 citedByCount "36" @default.
• W2314904034 countsByYear W23149040342015 @default.
• W2314904034 countsByYear W23149040342016 @default.
• W2314904034 countsByYear W23149040342017 @default.
• W2314904034 countsByYear W23149040342018 @default.
• W2314904034 countsByYear W23149040342019 @default.
• W2314904034 countsByYear W23149040342020 @default.
• W2314904034 countsByYear W23149040342021 @default.
• W2314904034 countsByYear W23149040342022 @default.
• W2314904034 countsByYear W23149040342023 @default.
• W2314904034 crossrefType "journal-article" @default.
• W2314904034 hasAuthorship W2314904034A5017633424 @default.
• W2314904034 hasAuthorship W2314904034A5032630657 @default.
• W2314904034 hasAuthorship W2314904034A5038378682 @default.
• W2314904034 hasAuthorship W2314904034A5052022866 @default.
• W2314904034 hasAuthorship W2314904034A5062539134 @default.
• W2314904034 hasAuthorship W2314904034A5068055890 @default.
• W2314904034 hasAuthorship W2314904034A5069065790 @default.
• W2314904034 hasAuthorship W2314904034A5070853499 @default.
• W2314904034 hasAuthorship W2314904034A5084647017 @default.
• W2314904034 hasConcept C10390740 @default.
• W2314904034 hasConcept C121332964 @default.
• W2314904034 hasConcept C162356407 @default.
• W2314904034 hasConcept C165697059 @default.
• W2314904034 hasConcept C171679685 @default.
• W2314904034 hasConcept C181199279 @default.
• W2314904034 hasConcept C185592680 @default.
• W2314904034 hasConcept C2777824588 @default.
• W2314904034 hasConcept C2778576202 @default.
• W2314904034 hasConcept C2779179908 @default.

• next