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• W2315143852 abstract "Event Abstract Back to Event Hydrophobized thermoresponsive polymer brush on porous monolithic silica for effective bioseparations Kenichi Nagase1, Jun Kobayashi1, Akihiko Kikuchi2, Yoshikatsu Akiyama1, Hideko Kanazawa3 and Teruo Okano1 1 Tokyo Women’s Medical University, Institute of Advanced Biomedical Engineering and Science, Japan 2 Tokyo University of Science, Department of Materials Science and Technology, Japan 3 Keio University, Faculty of Pharmacy, Japan Introduction: Thermoresponsive chromatography using poly(N-isopropylacrylamide)(PIPAAm) grafted silica beads have been investigated as new types of chromatography whose stationary phase property can be controlled simply by changing temperature[1]. For developing more efficient thermoresponsive chromatography system, we have prepared hydrophobized thermoresponsive copolymer modified monolithic silica column, and investigated their properties as chromatographic materials. Materials and Methods: ((Chloromethyl)phenylethyl)trimethoxysilane (CPTMS), ATRP-initiator, was immobilized on monolithic silica surfaces by flowing a toluene solution containing ATRP-initiator into monolithic silica columns using a HPLC pump. Copolymerization of IPAAm and n-butyl methacrylate (BMA) were performed onto monolithic-silica-rod surfaces through surface-initiated ATRP using CuCl/CuCl2/Me6TREN catalyst and 2-propanol as a reaction solution at 25 °C for 4 and 16 h (Fig.1). The prepared copolymer brush modified monolithic silica column was connected to an HPLC system, and the elution behaviour of benzoic acids and insulin fragments were observed at various temperatures. For the comparison, same copolymer brush modified silica beads packed column was prepared. Results and Discussion: CHN elemental analysis and GPC measurement revealed that grafted amount and grafted density of P(IPAAm-co-BMA) a on monolithic silica were 5.87 mg/m2 and 0.116 chains/nm2, respectively. These indicated that large amount of copolymer was grafted on monolithic silica surface. Fig.2 shows temperature dependent elution profiles of benzoic acids and phenol on the prepared column. Separation was performed at high temperature, because hydrophobic interaction between analytes and grafted copolymer enhanced at high temperature due to the dehydration of the copolymer. Monolithic silica column exhibit high-resolution separation of benzoic acids group compared to beads packed column. Fig.3 shows elution profile of insulin fragments. Monolithic silica columns performed baseline separation of insulin fragment. These results indicated that thermoresponsive hydrophobic monolithic silica column would be useful for bioseparation application. Conclusions: Hydrophobized thermoresponsive copolymer brush modified porous monolithic silica would be useful for effective bioseparation simply by changing column temperature. References:[1] K. Nagase, J. Kobayashi, T. Okano. Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering. J. R. Soc. Interface 2009, 6 (Suppl 3), S293-S309. Keywords: biosensing, Surface modification, polymer brush, stimuli-response Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biosensors Citation: Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H and Okano T (2016). Hydrophobized thermoresponsive polymer brush on porous monolithic silica for effective bioseparations. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02601 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kenichi Nagase Jun Kobayashi Akihiko Kikuchi Yoshikatsu Akiyama Hideko Kanazawa Teruo Okano Google Kenichi Nagase Jun Kobayashi Akihiko Kikuchi Yoshikatsu Akiyama Hideko Kanazawa Teruo Okano Google Scholar Kenichi Nagase Jun Kobayashi Akihiko Kikuchi Yoshikatsu Akiyama Hideko Kanazawa Teruo Okano PubMed Kenichi Nagase Jun Kobayashi Akihiko Kikuchi Yoshikatsu Akiyama Hideko Kanazawa Teruo Okano Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2315143852 title "Hydrophobized thermoresponsive polymer brush on porous monolithic silica for effective bioseparations" @default.
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