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- W2315172608 abstract "The amounts of TXB2 and PGD2 which were produced during spontaneous platelet aggregation (SPA) in 3 patients with primary thrombocythemia (PTH) were measured. After platelet counts in platelet-rich plasma (PRP) were adjusted to 120×104μl with autologous platelet-poor plasma, SPA was induced by stirring at 1100 r. p. m. in a Lumi-aggregometer and the lag time was measured. The amounts of TXB2 and PGD2 produced in the aliquots of PRP were measured by HPLC, using 9-anthryl-diazomethane. As a control, the amounts of TXB2 and PGD2 which were produced by the reaction of platelets with arachidonate (0.2mM, f. c.) were measured by similar method, using the platelets from 4 normal subjects (platelet count: 30×104μl).The amounts of TXB2 and PGD2 in normal subjects were 153.0±18.9, and 2.88±0.36ng/108 platelets (mean±S. D.), respectively (TXB2/D2=43.4 to 68.3). The amounts of TXB2 and PGD2 in PTH were as follows: case 1, 48.5 and 0.37; case 2, 56.2 and 1.14; case 3, 53.6 and 0.93 (ng/108 platelets), respectively (TXB2/D2=131.1, 49.3, 57.6). In case 1, the amount of PGD2 was decreased, and TXB2/D2 was significantly increased. In this case, it was also found that SPA occured in a shorter lag time than in the other cases. It was suggested that the increased TXB2/D2 may play some role in the mechanism of SPA in patients with PTH." @default.
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- W2315172608 date "1985-01-01" @default.
- W2315172608 modified "2023-10-02" @default.
- W2315172608 title "Analysis of prostaglandins induced by spontaneous platelet aggregation in patients with primary thrombocythemia." @default.
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- W2315172608 doi "https://doi.org/10.2491/jjsth1970.16.169" @default.
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