Matches in SemOpenAlex for { <https://semopenalex.org/work/W2315294164> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2315294164 abstract "A human melanoma cell line (Bowes) secretes tissue-type plasminogen activator (t-PA). In order to elucidate the mechanism of t-PA secretion, we analyzed intracellular distribution of t-PA by fractionating cell organelles of melanoma cells with discontinuous sucrose density gradient ultracentrifugation method, and investigated the effect of monensin, a secretion depressing agent, on the intracellular distribution of t-PA. The t-PA was determined by the radioactivity adsorbed to anti-t-PA IgG-Protein A-Sepharose. The distribution of plasminogen activator (PA) activity was similar to that of cellular protein concentration when they were separated by the ultracentrifugation. Enzymography showed that the major intracellular PA activity was present at 72kDa and the minor, activity at 50kDa. When t-PA secretion was depressed by monensin, intracellular PA was accumulated, especially the 72kDa component predominantly. After longterm (3h) simultaneous labeling with 35S-methionine and 3H-mannose, the 3H/35S ratio of t-PA increased in the presence of monensin. Pulse labeling experiments showed that intracellular t-PA was transported from the high density fraction to the low density one and reached a maximum concentration at 30min. Labeled t-PA began to appear in the culture medium in about 30min. These results suggest that t-PA secretion mechanism is similar to that of other secretory glycoproteins and that about 30 minutes are necessary from the ingestion of methionine to the secretion of t-PA." @default.
- W2315294164 created "2016-06-24" @default.
- W2315294164 creator A5009408667 @default.
- W2315294164 creator A5032308192 @default.
- W2315294164 creator A5059143404 @default.
- W2315294164 creator A5059629295 @default.
- W2315294164 creator A5074624990 @default.
- W2315294164 creator A5091428337 @default.
- W2315294164 date "1988-01-01" @default.
- W2315294164 modified "2023-09-23" @default.
- W2315294164 title "Intracellular distribution of tissue-type plasminogen activator in human melanoma cells." @default.
- W2315294164 cites W1540618467 @default.
- W2315294164 cites W1972905300 @default.
- W2315294164 cites W2001583712 @default.
- W2315294164 cites W2006943139 @default.
- W2315294164 cites W2014281268 @default.
- W2315294164 cites W2037051479 @default.
- W2315294164 cites W2056917962 @default.
- W2315294164 cites W2068507644 @default.
- W2315294164 cites W2106141363 @default.
- W2315294164 cites W2410896687 @default.
- W2315294164 doi "https://doi.org/10.2491/jjsth1970.19.107" @default.
- W2315294164 hasPublicationYear "1988" @default.
- W2315294164 type Work @default.
- W2315294164 sameAs 2315294164 @default.
- W2315294164 citedByCount "0" @default.
- W2315294164 crossrefType "journal-article" @default.
- W2315294164 hasAuthorship W2315294164A5009408667 @default.
- W2315294164 hasAuthorship W2315294164A5032308192 @default.
- W2315294164 hasAuthorship W2315294164A5059143404 @default.
- W2315294164 hasAuthorship W2315294164A5059629295 @default.
- W2315294164 hasAuthorship W2315294164A5074624990 @default.
- W2315294164 hasAuthorship W2315294164A5091428337 @default.
- W2315294164 hasBestOaLocation W23152941641 @default.
- W2315294164 hasConcept C134018914 @default.
- W2315294164 hasConcept C153911025 @default.
- W2315294164 hasConcept C185592680 @default.
- W2315294164 hasConcept C2779679481 @default.
- W2315294164 hasConcept C2780224938 @default.
- W2315294164 hasConcept C49039625 @default.
- W2315294164 hasConcept C54689828 @default.
- W2315294164 hasConcept C55493867 @default.
- W2315294164 hasConcept C79879829 @default.
- W2315294164 hasConcept C86803240 @default.
- W2315294164 hasConceptScore W2315294164C134018914 @default.
- W2315294164 hasConceptScore W2315294164C153911025 @default.
- W2315294164 hasConceptScore W2315294164C185592680 @default.
- W2315294164 hasConceptScore W2315294164C2779679481 @default.
- W2315294164 hasConceptScore W2315294164C2780224938 @default.
- W2315294164 hasConceptScore W2315294164C49039625 @default.
- W2315294164 hasConceptScore W2315294164C54689828 @default.
- W2315294164 hasConceptScore W2315294164C55493867 @default.
- W2315294164 hasConceptScore W2315294164C79879829 @default.
- W2315294164 hasConceptScore W2315294164C86803240 @default.
- W2315294164 hasLocation W23152941641 @default.
- W2315294164 hasOpenAccess W2315294164 @default.
- W2315294164 hasPrimaryLocation W23152941641 @default.
- W2315294164 hasRelatedWork W1520114847 @default.
- W2315294164 hasRelatedWork W1543912872 @default.
- W2315294164 hasRelatedWork W1561809002 @default.
- W2315294164 hasRelatedWork W1576136913 @default.
- W2315294164 hasRelatedWork W1862006453 @default.
- W2315294164 hasRelatedWork W1988211661 @default.
- W2315294164 hasRelatedWork W2013896081 @default.
- W2315294164 hasRelatedWork W2042322786 @default.
- W2315294164 hasRelatedWork W2077354385 @default.
- W2315294164 hasRelatedWork W2081392896 @default.
- W2315294164 hasRelatedWork W2088391438 @default.
- W2315294164 hasRelatedWork W2113623702 @default.
- W2315294164 hasRelatedWork W2135451833 @default.
- W2315294164 hasRelatedWork W2140407719 @default.
- W2315294164 hasRelatedWork W2284558072 @default.
- W2315294164 hasRelatedWork W2337271711 @default.
- W2315294164 hasRelatedWork W2413940356 @default.
- W2315294164 hasRelatedWork W38643905 @default.
- W2315294164 hasRelatedWork W48043969 @default.
- W2315294164 hasRelatedWork W78369820 @default.
- W2315294164 isParatext "false" @default.
- W2315294164 isRetracted "false" @default.
- W2315294164 magId "2315294164" @default.
- W2315294164 workType "article" @default.