Matches in SemOpenAlex for { <https://semopenalex.org/work/W2315340842> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2315340842 abstract "ELISA assays are broadly used as a diagnostic tool of paratuberculosis. Nevertheless, probl ems of specificity are due to the great number of shared antigens between MAP and other Mycobacteria , especially the closely related M. avium. An M. phlei preabsortion step in the ELISA eliminates much of the cross - reacting antibody due to environmental ba cteria. Despite this, other infections such as bovine tuberculosis, a frequent occurring disease in Brazil and in many other developing countries, may interfere with the specificity of the test. In this study, 97 sera from M. bovis infected cows that were skin - test reactive and confirmed as infected by histopathology. Sera were tested in a PPA (paratuberculosis protoplasmatic antigen – Allied Monitor, USA) ELISA that is routinely used for the diagnosis of paratuberculosis. From the 97 sera 25 (26.59%) pre sented an OD >0.60; the cut - off point for this test was standardized at OD 0.35. From these, twenty sera were randomly chosen for immunoblot analysis. Eighteen sera (90%) strongly reacted with four PPA bands: one 32 - 36KDa, one 42KDa and two others between 77 and 100KDa. An ELISA - positive control serum sample from a confirmed case of Johne’s disease from a region free of tuberculosis also recognized these same four bands. The findings indicate that the proteins are shared between M. bovis and MAP . In conclu sion, although ELISA tests may be used as valuable tools for the detection of paratuberculosis, interpretation of results in herds known to be infected with M. bovis should be done carefully." @default.
- W2315340842 created "2016-06-24" @default.
- W2315340842 creator A5031415152 @default.
- W2315340842 creator A5031909560 @default.
- W2315340842 creator A5041928431 @default.
- W2315340842 creator A5047484098 @default.
- W2315340842 creator A5057605713 @default.
- W2315340842 creator A5081522524 @default.
- W2315340842 date "2005-01-01" @default.
- W2315340842 modified "2023-09-25" @default.
- W2315340842 title "Interference of anti - M. bovis antibodies in serological tests for paratuberculosis" @default.
- W2315340842 cites W1904016582 @default.
- W2315340842 cites W2015945351 @default.
- W2315340842 cites W2041416187 @default.
- W2315340842 cites W2069701693 @default.
- W2315340842 cites W2104611060 @default.
- W2315340842 cites W2147105516 @default.
- W2315340842 cites W2154979664 @default.
- W2315340842 cites W2161020837 @default.
- W2315340842 cites W2169706872 @default.
- W2315340842 cites W320747670 @default.
- W2315340842 hasPublicationYear "2005" @default.
- W2315340842 type Work @default.
- W2315340842 sameAs 2315340842 @default.
- W2315340842 citedByCount "0" @default.
- W2315340842 crossrefType "journal-article" @default.
- W2315340842 hasAuthorship W2315340842A5031415152 @default.
- W2315340842 hasAuthorship W2315340842A5031909560 @default.
- W2315340842 hasAuthorship W2315340842A5041928431 @default.
- W2315340842 hasAuthorship W2315340842A5047484098 @default.
- W2315340842 hasAuthorship W2315340842A5057605713 @default.
- W2315340842 hasAuthorship W2315340842A5081522524 @default.
- W2315340842 hasConcept C142724271 @default.
- W2315340842 hasConcept C147483822 @default.
- W2315340842 hasConcept C159047783 @default.
- W2315340842 hasConcept C159654299 @default.
- W2315340842 hasConcept C194775826 @default.
- W2315340842 hasConcept C203014093 @default.
- W2315340842 hasConcept C2777849792 @default.
- W2315340842 hasConcept C2777975735 @default.
- W2315340842 hasConcept C2778497218 @default.
- W2315340842 hasConcept C2780374374 @default.
- W2315340842 hasConcept C2781069245 @default.
- W2315340842 hasConcept C42972112 @default.
- W2315340842 hasConcept C45189115 @default.
- W2315340842 hasConcept C71924100 @default.
- W2315340842 hasConcept C86803240 @default.
- W2315340842 hasConceptScore W2315340842C142724271 @default.
- W2315340842 hasConceptScore W2315340842C147483822 @default.
- W2315340842 hasConceptScore W2315340842C159047783 @default.
- W2315340842 hasConceptScore W2315340842C159654299 @default.
- W2315340842 hasConceptScore W2315340842C194775826 @default.
- W2315340842 hasConceptScore W2315340842C203014093 @default.
- W2315340842 hasConceptScore W2315340842C2777849792 @default.
- W2315340842 hasConceptScore W2315340842C2777975735 @default.
- W2315340842 hasConceptScore W2315340842C2778497218 @default.
- W2315340842 hasConceptScore W2315340842C2780374374 @default.
- W2315340842 hasConceptScore W2315340842C2781069245 @default.
- W2315340842 hasConceptScore W2315340842C42972112 @default.
- W2315340842 hasConceptScore W2315340842C45189115 @default.
- W2315340842 hasConceptScore W2315340842C71924100 @default.
- W2315340842 hasConceptScore W2315340842C86803240 @default.
- W2315340842 hasLocation W23153408421 @default.
- W2315340842 hasOpenAccess W2315340842 @default.
- W2315340842 hasPrimaryLocation W23153408421 @default.
- W2315340842 hasRelatedWork W1543778645 @default.
- W2315340842 hasRelatedWork W1969044514 @default.
- W2315340842 hasRelatedWork W1974931366 @default.
- W2315340842 hasRelatedWork W1998502187 @default.
- W2315340842 hasRelatedWork W2021319461 @default.
- W2315340842 hasRelatedWork W2029611092 @default.
- W2315340842 hasRelatedWork W2057538601 @default.
- W2315340842 hasRelatedWork W2089525028 @default.
- W2315340842 hasRelatedWork W2095028339 @default.
- W2315340842 hasRelatedWork W2127148933 @default.
- W2315340842 hasRelatedWork W2152921773 @default.
- W2315340842 hasRelatedWork W2154979664 @default.
- W2315340842 hasRelatedWork W2159828852 @default.
- W2315340842 hasRelatedWork W2325792250 @default.
- W2315340842 hasRelatedWork W2355753295 @default.
- W2315340842 hasRelatedWork W2413406351 @default.
- W2315340842 hasRelatedWork W2740640516 @default.
- W2315340842 hasRelatedWork W3107941542 @default.
- W2315340842 hasRelatedWork W320747670 @default.
- W2315340842 hasRelatedWork W6368872 @default.
- W2315340842 isParatext "false" @default.
- W2315340842 isRetracted "false" @default.
- W2315340842 magId "2315340842" @default.
- W2315340842 workType "article" @default.