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• W2315437781 abstract "The traditional medicine involves the use of different plant extracts or the bioactive constituents. This type of study provides the health application at affordable cost. Secondary metabolites are responsible for medicinal activity of plants. Hence in the present study phytochemical screening of some important medicinal plants was carried out. Qualitative phytochemical analysis of these plants confirms the presence of various phytochemicals like alkaloids, terpenoids, saponins, steroids, anthocyanin and tannins. The results suggest that the phytochemicals with medicinal properties can be used for curing various ailments and possess potential antioxidant and leads to the isolation of new and novel compounds. Also the study of antibacterial activity against Bacillus substilis and Staphylococcus aureus shows the resistivity power of the plants against them. INTRODUCTION Humans, since ancient times, have been exploiting the nature, particularly plants in search of new drugs. This has resulting in the use of large number of medicinal plants with curative properties to treat various diseases. Nearly 80% of the world’s population relies on traditional medicines for primary health care, most of which involve the use of plant extracts. In India, almost 95% of the prescriptions are plant based in the traditional systems of Unani, Ayurveda, Homeopathy and Siddha. The study of plants continues principally for the discovery of novel secondary metabolites. Around 80% of products are of plant origin. Plant products have been part of phytomedicines since time immemorial. These can be derived from any part of the plant like bark, leaves, flowers, seeds, roots etc. which contains active components. Knowledge of the chemical constituents of plants is desirable because such information will be of value for the synthesis of complex chemical substances. Such phytochemical screening of various plants is reported by many workers. In the present work, qualitative phytochemical analysis of 25 medicinal plants is been carried. MATERIALS AND METHODS Plant materials In August 2013 the plant materials was collected from the botanical garden of Padmashri Vikhe Patil College of Arts, Science and Commerce, Pravaranagar. These plants were identified with the help of the available literature and were authenticated by Dr. K. J. Salunke and Dr. A. S. Wabale, Department of Botany, Padmashri Vikhe Patil College of Arts, Science and Commerce, Pravaranagar. The collected parts of the medicinal plants were cleaned, air-dried in the shade and grinded into fine powder. Extraction The leaves were washed thoroughly 2-3 times with running tap water, then air dried under shade and the plant material was grinded in mixer. The powder was kept in small plastic bags with paper labeling. The grinded leaf material of 5gm was weighed using an electronic balance and were crushed in 25 ml of distilled water and kept on shaker for overnight and was filtered through Whatman No.1 filter paper. The filtrate was stored at room temperature. Phytochemical Screening Qualitative phytochemical screening was carried out with the following:• Steroids: 1 ml of the extract was dissolved in 10 ml of chloroform and equal volume of concentrated sulphuric acid was added to the test tube. • Terpenoids: 2 ml of extract was added to 2 ml of acetic anhydride and concentration of H2SO4. • Tannins: 2 ml of extract was added to few drops of 10% ferric chloride. • Saponins: 5 ml of extract was mixed with 20 ml of distilled water and then agitated in a graduated cylinder for 15 minutes. • Alkaloids: 2 ml of extract was mixed with 3ml of Dilute HCL and placed in steam bath for 5 min. Then add 1 ml of Dragendoff’s reagent. • Phlobatannin: 1 ml of extract was boiled in 2ml of 1% aq. HCL. • Quinone: 1 ml of extract was mixed with 1 ml of concentrated sulphuric acid. • Glycosides: 2 ml of extract was mixed with 3 ml of chloroform and 1 ml 10 % ammonia solution was added. • Flavanoids: 3 ml of 1 % aluminium chloride solution was added to 5 ml of extract. Testing for Antibacterial properties of plant extracts: Agar Well Diffusion Method was used for testing the antibacterial properties of selected medicinal plants. Nutrient agar plates were inoculated with 100 μl of inoculums. Wells were created using cork borer with 6 mm diameter and filled with 100 μl of plant extracts. Plates seeded with bacteria were allowed to incubate at 37°C. Zones of inhibition were measured after 24 hours of incubation (J. Parekh et al, 2006, Dhia Hassawi et al, 2006). The contents of nutrient agar" @default.
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• W2315437781 date "2011-10-01" @default.
• W2315437781 modified "2023-09-23" @default.
• W2315437781 title "Phytochemical Screening of Selected Medicinal Plants and their Antibacterial Activities Against Bacillus Substilis and Staphylococcus Aureus" @default.
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• W2315437781 doi "https://doi.org/10.15373/2249555x/july2014/156" @default.
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