FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2315503416> ?p ?o ?g. }

• W2315503416 endingPage "3896" @default.
• W2315503416 startingPage "3890" @default.
• W2315503416 abstract "Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content cell-based assays. However, maximizing the value of microfluidic assays for applications such as drug discovery, screening, or toxicity evaluation will require assurance of within-device repeatability, day-to-day reproducibility, and robustness to variations in conditions that might occur from laboratory to laboratory. This report describes a study of the performance and variability of a cell-based toxicity assay in microfluidic devices made of poly(dimethylsiloxane) (PDMS). The assay involves expression of destabilized green fluorescent protein (GFP) as a reporter of intracellular protein synthesis and degradation. Reduction in cellular GFP due to inhibition of ribosome activity by cycloheximide (CHX) was quantified with real-time quantitative fluorescence imaging. Assay repeatability was measured within a 64-chamber microfluidic device. Assay performance across a range of cell loading densities within a single device was assessed, as was replication of measurements in microfluidic devices prepared on different days. Assay robustness was tested using different fluorescence illumination sources and reservoir-to-device tubing choices. Both microfluidic and larger scale assay conditions showed comparable GFP decay rates upon CHX exposure, but the microfluidic data provided the higher level of confidence." @default.
• W2315503416 created "2016-06-24" @default.
• W2315503416 creator A5012140890 @default.
• W2315503416 creator A5015219111 @default.
• W2315503416 creator A5034362497 @default.
• W2315503416 date "2011-04-20" @default.
• W2315503416 modified "2023-10-05" @default.
• W2315503416 title "Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay" @default.
• W2315503416 cites W1970282054 @default.
• W2315503416 cites W1970622583 @default.
• W2315503416 cites W1971500740 @default.
• W2315503416 cites W1980224338 @default.
• W2315503416 cites W1990017178 @default.
• W2315503416 cites W2012867417 @default.
• W2315503416 cites W2016915024 @default.
• W2315503416 cites W2017656225 @default.
• W2315503416 cites W2018950317 @default.
• W2315503416 cites W2029430860 @default.
• W2315503416 cites W2033117797 @default.
• W2315503416 cites W2041612826 @default.
• W2315503416 cites W2056937003 @default.
• W2315503416 cites W2066011027 @default.
• W2315503416 cites W2068962163 @default.
• W2315503416 cites W2073137330 @default.
• W2315503416 cites W2074381872 @default.
• W2315503416 cites W2077248355 @default.
• W2315503416 cites W2078782272 @default.
• W2315503416 cites W2086187432 @default.
• W2315503416 cites W2086428332 @default.
• W2315503416 cites W2090383796 @default.
• W2315503416 cites W2092941484 @default.
• W2315503416 cites W2099423350 @default.
• W2315503416 cites W2099636196 @default.
• W2315503416 cites W2125936195 @default.
• W2315503416 cites W2161720159 @default.
• W2315503416 cites W4239637714 @default.
• W2315503416 doi "https://doi.org/10.1021/ac200273f" @default.
• W2315503416 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21506521" @default.
• W2315503416 hasPublicationYear "2011" @default.
• W2315503416 type Work @default.
• W2315503416 sameAs 2315503416 @default.
• W2315503416 citedByCount "34" @default.
• W2315503416 countsByYear W23155034162012 @default.
• W2315503416 countsByYear W23155034162013 @default.
• W2315503416 countsByYear W23155034162014 @default.
• W2315503416 countsByYear W23155034162015 @default.
• W2315503416 countsByYear W23155034162017 @default.
• W2315503416 countsByYear W23155034162018 @default.
• W2315503416 countsByYear W23155034162019 @default.
• W2315503416 countsByYear W23155034162021 @default.
• W2315503416 countsByYear W23155034162023 @default.
• W2315503416 crossrefType "journal-article" @default.
• W2315503416 hasAuthorship W2315503416A5012140890 @default.
• W2315503416 hasAuthorship W2315503416A5015219111 @default.
• W2315503416 hasAuthorship W2315503416A5034362497 @default.
• W2315503416 hasConcept C104317684 @default.
• W2315503416 hasConcept C121332964 @default.
• W2315503416 hasConcept C136229726 @default.
• W2315503416 hasConcept C142613039 @default.
• W2315503416 hasConcept C1491633281 @default.
• W2315503416 hasConcept C154020017 @default.
• W2315503416 hasConcept C171250308 @default.
• W2315503416 hasConcept C185592680 @default.
• W2315503416 hasConcept C192562407 @default.
• W2315503416 hasConcept C2778128677 @default.
• W2315503416 hasConcept C2988198817 @default.
• W2315503416 hasConcept C3675279 @default.
• W2315503416 hasConcept C43617362 @default.
• W2315503416 hasConcept C53227056 @default.
• W2315503416 hasConcept C55493867 @default.
• W2315503416 hasConcept C62520636 @default.
• W2315503416 hasConcept C63479239 @default.
• W2315503416 hasConcept C71924100 @default.
• W2315503416 hasConcept C8673954 @default.
• W2315503416 hasConcept C91881484 @default.
• W2315503416 hasConcept C9893847 @default.
• W2315503416 hasConceptScore W2315503416C104317684 @default.
• W2315503416 hasConceptScore W2315503416C121332964 @default.
• W2315503416 hasConceptScore W2315503416C136229726 @default.
• W2315503416 hasConceptScore W2315503416C142613039 @default.
• W2315503416 hasConceptScore W2315503416C1491633281 @default.
• W2315503416 hasConceptScore W2315503416C154020017 @default.
• W2315503416 hasConceptScore W2315503416C171250308 @default.
• W2315503416 hasConceptScore W2315503416C185592680 @default.
• W2315503416 hasConceptScore W2315503416C192562407 @default.
• W2315503416 hasConceptScore W2315503416C2778128677 @default.
• W2315503416 hasConceptScore W2315503416C2988198817 @default.
• W2315503416 hasConceptScore W2315503416C3675279 @default.
• W2315503416 hasConceptScore W2315503416C43617362 @default.
• W2315503416 hasConceptScore W2315503416C53227056 @default.
• W2315503416 hasConceptScore W2315503416C55493867 @default.
• W2315503416 hasConceptScore W2315503416C62520636 @default.
• W2315503416 hasConceptScore W2315503416C63479239 @default.
• W2315503416 hasConceptScore W2315503416C71924100 @default.
• W2315503416 hasConceptScore W2315503416C8673954 @default.
• W2315503416 hasConceptScore W2315503416C91881484 @default.
• W2315503416 hasConceptScore W2315503416C9893847 @default.
• W2315503416 hasIssue "10" @default.

• next