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- W2315522702 endingPage "1621" @default.
- W2315522702 startingPage "1614" @default.
- W2315522702 abstract "The amplified, highly sensitive detection of DNA using the dendritic rolling circle amplification (RCA) is introduced. The analytical platform includes a circular DNA and a structurally tailored hairpin structure. The circular nucleic acid template includes a recognition sequence for the analyte DNA (the Tay-Sachs mutant gene), a complementary sequence to the Mg2+-dependent DNAzyme, and a sequence identical to the loop region of the coadded hairpin structure. The functional hairpin in the system consists of the analyte-sequence that is caged in the stem region and a single-stranded loop domain that communicates with the RCA product. The analyte activates the RCA process, leading to DNA chains consisting of the Mg2+-dependent DNAzyme and sequences that are complementary to the loop of the functional hairpin structure. Opening of the coadded hairpin releases the caged analyte sequence, resulting in the dendritic RCA-induced synthesis of the Mg2+-dependent DNAzyme units. The DNAzyme-catalyzed cleavage of a fluorophore/quencher-modified substrate leads to a fluorescence readout signal. The method enabled the analysis of the target DNA with a detection limit corresponding to 1 aM. By the design of two different circular DNAs that include recognition sites for two different target genes, complementary sequences for two different Mg2+-dependent DNAzyme sequences and two different functional hairpin structures, the dendritic RCA-stimulated multiplexed analysis of two different genes is demonstrated. The amplified dendritic RCA detection of DNA is further implemented to yield the hemin/G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme as catalytic labels that provide colorimetric or chemiluminescent readout signals." @default.
- W2315522702 created "2016-06-24" @default.
- W2315522702 creator A5014000947 @default.
- W2315522702 creator A5028953532 @default.
- W2315522702 creator A5037842873 @default.
- W2315522702 creator A5052946108 @default.
- W2315522702 creator A5055413760 @default.
- W2315522702 date "2014-01-13" @default.
- W2315522702 modified "2023-10-17" @default.
- W2315522702 title "Amplified and Multiplexed Detection of DNA Using the Dendritic Rolling Circle Amplified Synthesis of DNAzyme Reporter Units" @default.
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