FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2315596361> ?p ?o ?g. }

• W2315596361 abstract "AB Archival samples represent a significant potential for genetic studies, particularly in severe diseases with risk of lethal outcome, such as in cancer. In this pilot study, we aimed to evaluate the usability of archival bone marrow smears and biopsies for DNA extraction and purification, whole genome amplification (WGA), multiple marker analysis including 10 short tandem repeats, and finally a comprehensive genotyping of 33,683 single nucleotide polymorphisms (SNPs) with multiplexed targeted next-generation sequencing. A total of 73 samples from 21 bone marrow smears and 13 bone marrow biopsies from 18 Danish and Norwegian childhood acute lymphoblastic leukemia patients were included and compared with corresponding blood samples. Samples were grouped according to the age of sample and whether WGA was performed or not. We found that measurements of DNA concentration after DNA extraction was dependent on detection method and that spectrophotometry overestimated DNA amount compared with fluorometry. In the short tandem repeat analysis, detection rate dropped slightly with longer fragments. After WGA, this drop was more pronounced. Samples stored for 0 to 3 years showed better results compared with samples stored for 4 to 10 years. Acceptable call rates for SNPs were detected for 7 of 42 archival samples. In conclusion, archival bone marrow samples are suitable for DNA extraction and multiple marker analysis, but WGA was less successful, especially when longer fragments were analyzed. Multiple SNP analysis seems feasible, but the method has to be further optimized." @default.
• W2315596361 created "2016-06-24" @default.
• W2315596361 creator A5000877934 @default.
• W2315596361 creator A5015433047 @default.
• W2315596361 creator A5016436495 @default.
• W2315596361 creator A5040730315 @default.
• W2315596361 creator A5048241259 @default.
• W2315596361 creator A5055424152 @default.
• W2315596361 creator A5074845542 @default.
• W2315596361 creator A5082379914 @default.
• W2315596361 creator A5083278291 @default.
• W2315596361 date "2015-01-01" @default.
• W2315596361 modified "2023-09-26" @default.
• W2315596361 title "Archival Bone Marrow Samples" @default.
• W2315596361 cites W1509637701 @default.
• W2315596361 cites W1530977725 @default.
• W2315596361 cites W1972881894 @default.
• W2315596361 cites W1981915061 @default.
• W2315596361 cites W1982069732 @default.
• W2315596361 cites W1987485833 @default.
• W2315596361 cites W1991898784 @default.
• W2315596361 cites W1995209649 @default.
• W2315596361 cites W2003615647 @default.
• W2315596361 cites W2006188256 @default.
• W2315596361 cites W2033675776 @default.
• W2315596361 cites W2037451457 @default.
• W2315596361 cites W2038127615 @default.
• W2315596361 cites W2055804326 @default.
• W2315596361 cites W2069114336 @default.
• W2315596361 cites W2071503124 @default.
• W2315596361 cites W2071818770 @default.
• W2315596361 cites W2076057372 @default.
• W2315596361 cites W2080716772 @default.
• W2315596361 cites W2089548232 @default.
• W2315596361 cites W2103441770 @default.
• W2315596361 cites W2108234281 @default.
• W2315596361 cites W2119180969 @default.
• W2315596361 cites W2134654916 @default.
• W2315596361 cites W2153364123 @default.
• W2315596361 cites W2157288072 @default.
• W2315596361 cites W2165309322 @default.
• W2315596361 cites W2168917479 @default.
• W2315596361 doi "https://doi.org/10.1097/pdm.0000000000000032" @default.
• W2315596361 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25621358" @default.
• W2315596361 hasPublicationYear "2015" @default.
• W2315596361 type Work @default.
• W2315596361 sameAs 2315596361 @default.
• W2315596361 citedByCount "1" @default.
• W2315596361 countsByYear W23155963612017 @default.
• W2315596361 crossrefType "journal-article" @default.
• W2315596361 hasAuthorship W2315596361A5000877934 @default.
• W2315596361 hasAuthorship W2315596361A5015433047 @default.
• W2315596361 hasAuthorship W2315596361A5016436495 @default.
• W2315596361 hasAuthorship W2315596361A5040730315 @default.
• W2315596361 hasAuthorship W2315596361A5048241259 @default.
• W2315596361 hasAuthorship W2315596361A5055424152 @default.
• W2315596361 hasAuthorship W2315596361A5074845542 @default.
• W2315596361 hasAuthorship W2315596361A5082379914 @default.
• W2315596361 hasAuthorship W2315596361A5083278291 @default.
• W2315596361 hasConcept C104317684 @default.
• W2315596361 hasConcept C135763542 @default.
• W2315596361 hasConcept C142724271 @default.
• W2315596361 hasConcept C153209595 @default.
• W2315596361 hasConcept C153911025 @default.
• W2315596361 hasConcept C180754005 @default.
• W2315596361 hasConcept C2780007613 @default.
• W2315596361 hasConcept C31467283 @default.
• W2315596361 hasConcept C49105822 @default.
• W2315596361 hasConcept C54355233 @default.
• W2315596361 hasConcept C552990157 @default.
• W2315596361 hasConcept C61320498 @default.
• W2315596361 hasConcept C71924100 @default.
• W2315596361 hasConcept C78063203 @default.
• W2315596361 hasConcept C86803240 @default.
• W2315596361 hasConceptScore W2315596361C104317684 @default.
• W2315596361 hasConceptScore W2315596361C135763542 @default.
• W2315596361 hasConceptScore W2315596361C142724271 @default.
• W2315596361 hasConceptScore W2315596361C153209595 @default.
• W2315596361 hasConceptScore W2315596361C153911025 @default.
• W2315596361 hasConceptScore W2315596361C180754005 @default.
• W2315596361 hasConceptScore W2315596361C2780007613 @default.
• W2315596361 hasConceptScore W2315596361C31467283 @default.
• W2315596361 hasConceptScore W2315596361C49105822 @default.
• W2315596361 hasConceptScore W2315596361C54355233 @default.
• W2315596361 hasConceptScore W2315596361C552990157 @default.
• W2315596361 hasConceptScore W2315596361C61320498 @default.
• W2315596361 hasConceptScore W2315596361C71924100 @default.
• W2315596361 hasConceptScore W2315596361C78063203 @default.
• W2315596361 hasConceptScore W2315596361C86803240 @default.
• W2315596361 hasLocation W23155963611 @default.
• W2315596361 hasLocation W23155963612 @default.
• W2315596361 hasOpenAccess W2315596361 @default.
• W2315596361 hasPrimaryLocation W23155963611 @default.
• W2315596361 hasRelatedWork W18165771 @default.
• W2315596361 hasRelatedWork W2043295059 @default.
• W2315596361 hasRelatedWork W2075393343 @default.
• W2315596361 hasRelatedWork W2125835121 @default.
• W2315596361 hasRelatedWork W2152580372 @default.
• W2315596361 hasRelatedWork W2412999359 @default.
• W2315596361 hasRelatedWork W2750701901 @default.

• next