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- W2315672360 abstract "SummaryRat liver homogenate was separated into nuclei, mitochondria, microsomes and supernatant fractions by differential centrifugation. The homogenate, the fractions and combinations of fractions were studied for their ability to decrease the activity of estradiol, estrone or diethylstilbestrol when incubated at 38°C for 2 hours in the presence of DPN and nicotinamide. The uteri of ovariectomized mice were used to determine the amount of biological activity remaining.No single fraction decreased the activity of estradiol markedly. The microsomes and supernatant, when recombined, were comparable to homogenate in inactivating this hormone.Though the homogenate of kidney or salivary glands was inert in this respect, the supernatant of either tissue when combined with liver microsomes effectively inactivated estradiol. The supernatant could also be replaced by riboflavin monophosphate (RMP). The microsomes of kidney or of salivary glands when used with liver supernatant failed to inactivate estradiol. The i..." @default.
- W2315672360 created "2016-06-24" @default.
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- W2315672360 date "1952-08-01" @default.
- W2315672360 modified "2023-09-27" @default.
- W2315672360 title "Intracellular Distribution of Estrogen Inactivating Mechanism in Rat Liver and Other Tissues." @default.
- W2315672360 doi "https://doi.org/10.3181/00379727-80-19711" @default.
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