FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2315790926> ?p ?o ?g. }

• W2315790926 endingPage "15929" @default.
• W2315790926 startingPage "15915" @default.
• W2315790926 abstract "// Dennis Sohn 1 , Dominik Peters 1 , Roland P. Piekorz 2 , Wilfried Budach 1 , Reiner U. Jänicke 1 1 Laboratory of Molecular Radiooncology, Clinic and Policlinic for Radiation Therapy and Radiooncology, Medical Faculty of the Heinrich-Heine-University, Düsseldorf, Germany 2 Institute for Biochemistry and Molecular Biology II, Medical Faculty of the Heinrich-Heine-University, Düsseldorf, Germany Correspondence to: Reiner U. Jänicke, e-mail: janicke@uni-duesseldorf.de Keywords: apoptosis, senescence, microRNA-30e, p21, caspase-3 Received: October 01, 2015      Accepted: February 05, 2016      Published: February 17, 2016 ABSTRACT MicroRNAs (miRNAs), a class of small non-coding RNAs that usually cause gene silencing by translational repression or degradation of mRNAs, are implicated in DNA damage-induced stress responses. To identify senescence-associated miRNAs, we performed microarray analyses using wild-type and p53-deficient HCT116 colon carcinoma cells that following gamma-irradiation (γIR) are driven into senescence and apoptosis, respectively. Several miRNAs including miR-30e were found upregulated in a p53-dependent manner specifically in senescent cells, but not in apoptotic cells. Overexpression of miR-30e in HCT116 cells not only inhibited γIR-, etoposide- or miR-34a-induced caspase-3-like DEVDase activities and cell death, but greatly accelerated and augmented their senescent phenotype. Consistently, procaspase-3 protein, but not mRNA decreased in the presence of miR-30e, whereas expression of the cyclin-dependent kinase inhibitor p21 increased both at the mRNA and protein level. Performing luciferase reporter gene assays, we identified the 3’-UTR of the caspase-3 mRNA as a direct miR-30e target. In contrast, although miR-30e was unable to bind to the p21 mRNA, it increased expression of a luciferase construct containing the p21 promoter, suggesting that the miR-30e-mediated upregulation of p21 occurs indirectly at the transcriptional level. Interestingly, despite suppressing procaspase-3 expression, miR-30e was unable to protect RKO colon carcinoma cells from DNA damage-induced death or to induce senescence, as miR-30e completely fails to upregulate p21 in these cells. These data suggest that miR-30e functions in a cell type-dependent manner as an important molecular switch for DNA damage-induced stress responses and may thus represent a target of therapeutic value." @default.
• W2315790926 created "2016-06-24" @default.
• W2315790926 creator A5001313496 @default.
• W2315790926 creator A5036353375 @default.
• W2315790926 creator A5040511454 @default.
• W2315790926 creator A5055378278 @default.
• W2315790926 creator A5073827670 @default.
• W2315790926 date "2016-02-17" @default.
• W2315790926 modified "2023-10-18" @default.
• W2315790926 title "miR-30e controls DNA damage-induced stress responses by modulating expression of the CDK inhibitor p21WAF1/CIP1 and caspase-3" @default.
• W2315790926 cites W132441100 @default.
• W2315790926 cites W144423133 @default.
• W2315790926 cites W1521411817 @default.
• W2315790926 cites W1563316806 @default.
• W2315790926 cites W1650797637 @default.
• W2315790926 cites W1964398217 @default.
• W2315790926 cites W1964813626 @default.
• W2315790926 cites W1966269514 @default.
• W2315790926 cites W1969294374 @default.
• W2315790926 cites W1970431885 @default.
• W2315790926 cites W1973909437 @default.
• W2315790926 cites W1978337436 @default.
• W2315790926 cites W1982966355 @default.
• W2315790926 cites W1983266682 @default.
• W2315790926 cites W1988163167 @default.
• W2315790926 cites W1992686564 @default.
• W2315790926 cites W1997746865 @default.
• W2315790926 cites W2006128630 @default.
• W2315790926 cites W2006797972 @default.
• W2315790926 cites W2007463943 @default.
• W2315790926 cites W2010763857 @default.
• W2315790926 cites W2011528645 @default.
• W2315790926 cites W2014385820 @default.
• W2315790926 cites W2024241876 @default.
• W2315790926 cites W2024715737 @default.
• W2315790926 cites W2032412054 @default.
• W2315790926 cites W2037611415 @default.
• W2315790926 cites W2044532276 @default.
• W2315790926 cites W2044967196 @default.
• W2315790926 cites W2045563337 @default.
• W2315790926 cites W2045842462 @default.
• W2315790926 cites W2048893832 @default.
• W2315790926 cites W2050869737 @default.
• W2315790926 cites W2051088438 @default.
• W2315790926 cites W2052480299 @default.
• W2315790926 cites W2053086049 @default.
• W2315790926 cites W2054264611 @default.
• W2315790926 cites W2055917239 @default.
• W2315790926 cites W2063669247 @default.
• W2315790926 cites W2071603995 @default.
• W2315790926 cites W2072239590 @default.
• W2315790926 cites W2074466442 @default.
• W2315790926 cites W2075207273 @default.
• W2315790926 cites W2080125477 @default.
• W2315790926 cites W2083381199 @default.
• W2315790926 cites W2083822954 @default.
• W2315790926 cites W2084218374 @default.
• W2315790926 cites W2086424362 @default.
• W2315790926 cites W2089964771 @default.
• W2315790926 cites W2100473605 @default.
• W2315790926 cites W2102876017 @default.
• W2315790926 cites W2103211592 @default.
• W2315790926 cites W2107669965 @default.
• W2315790926 cites W2109261002 @default.
• W2315790926 cites W2113239055 @default.
• W2315790926 cites W2119337308 @default.
• W2315790926 cites W2120557342 @default.
• W2315790926 cites W2124808167 @default.
• W2315790926 cites W2130979840 @default.
• W2315790926 cites W2135836598 @default.
• W2315790926 cites W2143464168 @default.
• W2315790926 cites W2144169031 @default.
• W2315790926 cites W2146656223 @default.
• W2315790926 cites W2147318282 @default.
• W2315790926 cites W2159004696 @default.
• W2315790926 cites W2161820095 @default.
• W2315790926 cites W2162789670 @default.
• W2315790926 cites W2163188200 @default.
• W2315790926 cites W2164730704 @default.
• W2315790926 cites W2165997736 @default.
• W2315790926 cites W2168790441 @default.
• W2315790926 cites W4242740353 @default.
• W2315790926 cites W561446919 @default.
• W2315790926 doi "https://doi.org/10.18632/oncotarget.7432" @default.
• W2315790926 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4941286" @default.
• W2315790926 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26895377" @default.
• W2315790926 hasPublicationYear "2016" @default.
• W2315790926 type Work @default.
• W2315790926 sameAs 2315790926 @default.
• W2315790926 citedByCount "13" @default.
• W2315790926 countsByYear W23157909262016 @default.
• W2315790926 countsByYear W23157909262017 @default.
• W2315790926 countsByYear W23157909262018 @default.
• W2315790926 countsByYear W23157909262019 @default.
• W2315790926 countsByYear W23157909262022 @default.
• W2315790926 crossrefType "journal-article" @default.
• W2315790926 hasAuthorship W2315790926A5001313496 @default.
• W2315790926 hasAuthorship W2315790926A5036353375 @default.

• next