Matches in SemOpenAlex for { <https://semopenalex.org/work/W2315881010> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2315881010 endingPage "891" @default.
- W2315881010 startingPage "885" @default.
- W2315881010 abstract "We have isolated a human lymphoblastoid cell line with higher levels of native cytochrome P450IA1 activity and by DNA transfection introduced human cDNAs for a putative cytochrome P450IIA2 and epoxide hydrolase (E.C.3.3.2.3). The resultant cell line, designated MCL-1, was substantially more sensitive to the mutagenicity of dimethylnitrosamine and benzo[a]pyrene than the AHH-1 cell line and was found to have increased metabolism of benzo[a]pyrene to dihydrodiols. The increase in native cytochrome P450IA1 activity was achieved by mutation and selection based on resistance to the phototoxicity of benzo[ghi]perylene. One resistant clone, designated L3, was used for subsequent studies. Two complete cDNAs, one encoding a putative cytochrome P450IIA2 and the other a microsomal epoxide hydrolase, were isolated from a human liver cDNA library. After introduction of the cDNAs into an expression vector and transfection into AHH-1 cells, gene expression was detected at the level of enzyme activity (epoxide hydrolase) or by increased sensitivity to dimethyl-nitrosamine cytotoxicity/mutagenicity (putative P450IIA2). A vector containing both cDNAs was then constructed and transfected into L3 cells to produce MCL-1 cells. The potential usefulness of drug-metabolizing gene transfection and of the MCL-1 cell line, in particular, for genetic toxicity testing is discussed." @default.
- W2315881010 created "2016-06-24" @default.
- W2315881010 creator A5010022613 @default.
- W2315881010 creator A5030016727 @default.
- W2315881010 creator A5046101110 @default.
- W2315881010 creator A5057896673 @default.
- W2315881010 creator A5088332392 @default.
- W2315881010 date "1989-05-01" @default.
- W2315881010 modified "2023-09-24" @default.
- W2315881010 title "Development of a human cell line by selection and drug-metabolizing gene transfection with increased capacity to activate promutagens" @default.
- W2315881010 doi "https://doi.org/10.1093/carcin/10.5.885" @default.
- W2315881010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2706743" @default.
- W2315881010 hasPublicationYear "1989" @default.
- W2315881010 type Work @default.
- W2315881010 sameAs 2315881010 @default.
- W2315881010 citedByCount "59" @default.
- W2315881010 countsByYear W23158810102016 @default.
- W2315881010 crossrefType "journal-article" @default.
- W2315881010 hasAuthorship W2315881010A5010022613 @default.
- W2315881010 hasAuthorship W2315881010A5030016727 @default.
- W2315881010 hasAuthorship W2315881010A5046101110 @default.
- W2315881010 hasAuthorship W2315881010A5057896673 @default.
- W2315881010 hasAuthorship W2315881010A5088332392 @default.
- W2315881010 hasConcept C101819947 @default.
- W2315881010 hasConcept C104317684 @default.
- W2315881010 hasConcept C153911025 @default.
- W2315881010 hasConcept C181199279 @default.
- W2315881010 hasConcept C185592680 @default.
- W2315881010 hasConcept C187882448 @default.
- W2315881010 hasConcept C2776671928 @default.
- W2315881010 hasConcept C2780768313 @default.
- W2315881010 hasConcept C526171541 @default.
- W2315881010 hasConcept C54009773 @default.
- W2315881010 hasConcept C54355233 @default.
- W2315881010 hasConcept C55493867 @default.
- W2315881010 hasConcept C81885089 @default.
- W2315881010 hasConcept C86803240 @default.
- W2315881010 hasConcept C87644729 @default.
- W2315881010 hasConceptScore W2315881010C101819947 @default.
- W2315881010 hasConceptScore W2315881010C104317684 @default.
- W2315881010 hasConceptScore W2315881010C153911025 @default.
- W2315881010 hasConceptScore W2315881010C181199279 @default.
- W2315881010 hasConceptScore W2315881010C185592680 @default.
- W2315881010 hasConceptScore W2315881010C187882448 @default.
- W2315881010 hasConceptScore W2315881010C2776671928 @default.
- W2315881010 hasConceptScore W2315881010C2780768313 @default.
- W2315881010 hasConceptScore W2315881010C526171541 @default.
- W2315881010 hasConceptScore W2315881010C54009773 @default.
- W2315881010 hasConceptScore W2315881010C54355233 @default.
- W2315881010 hasConceptScore W2315881010C55493867 @default.
- W2315881010 hasConceptScore W2315881010C81885089 @default.
- W2315881010 hasConceptScore W2315881010C86803240 @default.
- W2315881010 hasConceptScore W2315881010C87644729 @default.
- W2315881010 hasIssue "5" @default.
- W2315881010 hasLocation W23158810101 @default.
- W2315881010 hasLocation W23158810102 @default.
- W2315881010 hasOpenAccess W2315881010 @default.
- W2315881010 hasPrimaryLocation W23158810101 @default.
- W2315881010 hasRelatedWork W1490685579 @default.
- W2315881010 hasRelatedWork W1997774836 @default.
- W2315881010 hasRelatedWork W1999774783 @default.
- W2315881010 hasRelatedWork W2031744249 @default.
- W2315881010 hasRelatedWork W2044836893 @default.
- W2315881010 hasRelatedWork W2045555964 @default.
- W2315881010 hasRelatedWork W2061895553 @default.
- W2315881010 hasRelatedWork W2166357188 @default.
- W2315881010 hasRelatedWork W2364319975 @default.
- W2315881010 hasRelatedWork W2412860237 @default.
- W2315881010 hasVolume "10" @default.
- W2315881010 isParatext "false" @default.
- W2315881010 isRetracted "false" @default.
- W2315881010 magId "2315881010" @default.
- W2315881010 workType "article" @default.