Matches in SemOpenAlex for { <https://semopenalex.org/work/W2316013803> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2316013803 endingPage "2954" @default.
- W2316013803 startingPage "2947" @default.
- W2316013803 abstract "The purpose of this study was to determine and evaluate the current status of antimicrobial resistance, both phenotypically and genotypically, of the most common aerobic pathogenic bacteria recovered from diabetic foot ulcers (DFUs) in Egypt. Standard methods were used for culture identification, sensitivity testing and extended spectrum β-lactamases (ESBLs) detection. PCR for bla genes was performed and the obtained PCR products were verified by DNA sequencing. A total of 206 clinical bacterial isolates were recovered from DFU specimens, of which 135 (65.5%) were Gram negative and 71 (34.5%) were Gram positive. Gram negative isolates were mainly Proteus spp. (49; 24.4%), Escherichia coli (24; 11.6%), Pseudomonas spp. (19; 9.2%) and Klebsiella spp. (17; 8.2%) while, Gram positive isolates were mostly Staphylococcus aureus (26; 12.6%) and coagulase-negative staphylococci (25; 12.13%). The antibiogram analysis of Gram negative isolates revealed a remarkable high resistance pattern towards most of the tested antibiotics particularly, 3rd, 4th generation cephalosporins and fluroquinolones. About, 50.87% of Gram negative isolates were ESBL producers of which 14% were plasmid-mediated. Upon molecular characterization of plasmid-mediated ESBLs by PCR, blaCTX-M showed 100% positivity, followed by blaTEM (50%) and blaSHV (37.5%). For further detection of variants within genes, only one E. coli isolate that harbored a plasmid coding for three genes was sent for DNA sequencing. The results revealed presence of blaTEM-1 (accession number JX976326), blaSHV-8 (accession number JX976327) and blaCTX-M on a single plasmid coded pECDF16 as deposited in GenBank. The majority of ESBLs recovered from DFUs, showed resistance to more than one class of antimicrobial agents and hence new guidelines should be addressed in Egypt to rationalize and prevent the misuse and overuse of antimicrobial agents. Key words: Diabetic foot ulcers, extended spectrum β-lactamase (ESBL), plasmid, blaCTX-M, blaTEM-1, blaSHV-8." @default.
- W2316013803 created "2016-06-24" @default.
- W2316013803 creator A5042021463 @default.
- W2316013803 creator A5051783132 @default.
- W2316013803 creator A5056888938 @default.
- W2316013803 creator A5090580441 @default.
- W2316013803 date "2014-07-30" @default.
- W2316013803 modified "2023-10-01" @default.
- W2316013803 title "Antibacterial resistance pattern of aerobic bacteria isolated from patients with diabetic foot ulcers in Egypt" @default.
- W2316013803 cites W1596764968 @default.
- W2316013803 cites W2019475128 @default.
- W2316013803 doi "https://doi.org/10.5897/ajmr2014.6909" @default.
- W2316013803 hasPublicationYear "2014" @default.
- W2316013803 type Work @default.
- W2316013803 sameAs 2316013803 @default.
- W2316013803 citedByCount "2" @default.
- W2316013803 countsByYear W23160138032020 @default.
- W2316013803 countsByYear W23160138032023 @default.
- W2316013803 crossrefType "journal-article" @default.
- W2316013803 hasAuthorship W2316013803A5042021463 @default.
- W2316013803 hasAuthorship W2316013803A5051783132 @default.
- W2316013803 hasAuthorship W2316013803A5056888938 @default.
- W2316013803 hasAuthorship W2316013803A5090580441 @default.
- W2316013803 hasBestOaLocation W23160138031 @default.
- W2316013803 hasConcept C104317684 @default.
- W2316013803 hasConcept C134018914 @default.
- W2316013803 hasConcept C22744801 @default.
- W2316013803 hasConcept C2777858829 @default.
- W2316013803 hasConcept C2779288629 @default.
- W2316013803 hasConcept C2779489039 @default.
- W2316013803 hasConcept C501593827 @default.
- W2316013803 hasConcept C523546767 @default.
- W2316013803 hasConcept C54355233 @default.
- W2316013803 hasConcept C555293320 @default.
- W2316013803 hasConcept C64778159 @default.
- W2316013803 hasConcept C86803240 @default.
- W2316013803 hasConcept C89423630 @default.
- W2316013803 hasConcept C94665300 @default.
- W2316013803 hasConceptScore W2316013803C104317684 @default.
- W2316013803 hasConceptScore W2316013803C134018914 @default.
- W2316013803 hasConceptScore W2316013803C22744801 @default.
- W2316013803 hasConceptScore W2316013803C2777858829 @default.
- W2316013803 hasConceptScore W2316013803C2779288629 @default.
- W2316013803 hasConceptScore W2316013803C2779489039 @default.
- W2316013803 hasConceptScore W2316013803C501593827 @default.
- W2316013803 hasConceptScore W2316013803C523546767 @default.
- W2316013803 hasConceptScore W2316013803C54355233 @default.
- W2316013803 hasConceptScore W2316013803C555293320 @default.
- W2316013803 hasConceptScore W2316013803C64778159 @default.
- W2316013803 hasConceptScore W2316013803C86803240 @default.
- W2316013803 hasConceptScore W2316013803C89423630 @default.
- W2316013803 hasConceptScore W2316013803C94665300 @default.
- W2316013803 hasIssue "31" @default.
- W2316013803 hasLocation W23160138031 @default.
- W2316013803 hasOpenAccess W2316013803 @default.
- W2316013803 hasPrimaryLocation W23160138031 @default.
- W2316013803 hasRelatedWork W2044265753 @default.
- W2316013803 hasRelatedWork W2085711542 @default.
- W2316013803 hasRelatedWork W2326830576 @default.
- W2316013803 hasRelatedWork W2345602924 @default.
- W2316013803 hasRelatedWork W2401291924 @default.
- W2316013803 hasRelatedWork W2418689723 @default.
- W2316013803 hasRelatedWork W3109929327 @default.
- W2316013803 hasRelatedWork W4205138535 @default.
- W2316013803 hasRelatedWork W4310874084 @default.
- W2316013803 hasRelatedWork W4310875765 @default.
- W2316013803 hasVolume "8" @default.
- W2316013803 isParatext "false" @default.
- W2316013803 isRetracted "false" @default.
- W2316013803 magId "2316013803" @default.
- W2316013803 workType "article" @default.