FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2316172686> ?p ?o ?g. }

• W2316172686 abstract "The homeobox transcription factor Hlxb9 controls pancreatic β-cell differentiation during the early and late stages of endocrine pancreas development. Recently, increased expression of Hlxb9 was observed in β-cell tumors associated with multiple endocrine neoplasia type 1 (MEN1), a tumor syndrome caused by germline mutation in the MEN1 tumor suppressor gene encoding menin. In MIN6 cells, a mouse pancreatic β-cell line, overexpression of Hlxb9 has been shown to cause apoptosis in a menin-dependent manner. Although Hlxb9 is known to act as a transcriptional repressor, its DNA-binding site at its target genes in pancreatic β-cells has not been elucidated. To identify targets of Hlxb9 and genomic binding sites in β-cells (MIN6 cells) we used chromatin immunoprecipitation and deep sequencing (ChIP-seq) followed by bioinformatics analysis of the ChIP-seq data. The Hlxb9 specific ChIP-seq libraries and corresponding input controls were sequenced on the Illumina Hi Seq 2000. The ChIP-seq reads were subjected to peak calling tools, and upon stringent filtering 2569 peaks were obtained. After assigning chromosomal positions to the peaks, 24 statistically significant (FDR-P de novo using the Genomatix Genome Analyzer suite that appeared to be common in most of the targets (Re-value: 1.88). Further analysis revealed that this motif was similar to binding sequences of the following proteins: Tcfe2a (involved in brain development) , Ascl2 (involved in nervous system) , REI1 (involved in mitotic signal network) , ZFp691 (zinc finger protein) , myf (involved in mitosis), and Bcl6b (involved in apoptosis). Gene Ontology analysis of the identified targets showed that 18.3% were associated with the Wnt signaling pathway, 27.3% with cell proliferation, 54.5% were involved in cellular response to stimulus, 13.6% in MAPK cascade and 31.8% in negative regulation of biological processes. Our genome-wide analysis of Hlxb9 target genes demonstrates the presence of a potential Hlxb9-binding site in target genes associated with important processes relevant for cellular differentiation and neoplasia. Further studies investigating these targets for menin-dependent and menin-independent actions of Hlxb9 will provide insights into the role of menin-Hlxb9 interaction in normal physiology and in the development of pancreatic β-cell tumors. Citation Format: Shruti S. Desai, Sita D. Modali, Weiping Chen, Vaishali I. Parekh, Sunita K. Agarwal. ChIP-seq based identification of genes regulated by a pancreatic β-cell differentiation factor, Hlxb9, that is dysregulated in β-cell tumors. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr LB-118. doi:10.1158/1538-7445.AM2013-LB-118" @default.
• W2316172686 created "2016-06-24" @default.
• W2316172686 creator A5026047135 @default.
• W2316172686 creator A5030764726 @default.
• W2316172686 creator A5031915018 @default.
• W2316172686 creator A5032079097 @default.
• W2316172686 creator A5048517621 @default.
• W2316172686 date "2013-04-15" @default.
• W2316172686 modified "2023-10-14" @default.
• W2316172686 title "Abstract LB-118: ChIP-seq based identification of genes regulated by a pancreatic β-cell differentiation factor, Hlxb9, that is dysregulated in β-cell tumors." @default.
• W2316172686 doi "https://doi.org/10.1158/1538-7445.am2013-lb-118" @default.
• W2316172686 hasPublicationYear "2013" @default.
• W2316172686 type Work @default.
• W2316172686 sameAs 2316172686 @default.
• W2316172686 citedByCount "0" @default.
• W2316172686 crossrefType "proceedings-article" @default.
• W2316172686 hasAuthorship W2316172686A5026047135 @default.
• W2316172686 hasAuthorship W2316172686A5030764726 @default.
• W2316172686 hasAuthorship W2316172686A5031915018 @default.
• W2316172686 hasAuthorship W2316172686A5032079097 @default.
• W2316172686 hasAuthorship W2316172686A5048517621 @default.
• W2316172686 hasConcept C101762097 @default.
• W2316172686 hasConcept C103395026 @default.
• W2316172686 hasConcept C104317684 @default.
• W2316172686 hasConcept C121587040 @default.
• W2316172686 hasConcept C134018914 @default.
• W2316172686 hasConcept C134320426 @default.
• W2316172686 hasConcept C150194340 @default.
• W2316172686 hasConcept C153911025 @default.
• W2316172686 hasConcept C191370860 @default.
• W2316172686 hasConcept C2778764654 @default.
• W2316172686 hasConcept C2779469564 @default.
• W2316172686 hasConcept C2780103800 @default.
• W2316172686 hasConcept C46699223 @default.
• W2316172686 hasConcept C502942594 @default.
• W2316172686 hasConcept C54355233 @default.
• W2316172686 hasConcept C71315377 @default.
• W2316172686 hasConcept C86339819 @default.
• W2316172686 hasConcept C86803240 @default.
• W2316172686 hasConceptScore W2316172686C101762097 @default.
• W2316172686 hasConceptScore W2316172686C103395026 @default.
• W2316172686 hasConceptScore W2316172686C104317684 @default.
• W2316172686 hasConceptScore W2316172686C121587040 @default.
• W2316172686 hasConceptScore W2316172686C134018914 @default.
• W2316172686 hasConceptScore W2316172686C134320426 @default.
• W2316172686 hasConceptScore W2316172686C150194340 @default.
• W2316172686 hasConceptScore W2316172686C153911025 @default.
• W2316172686 hasConceptScore W2316172686C191370860 @default.
• W2316172686 hasConceptScore W2316172686C2778764654 @default.
• W2316172686 hasConceptScore W2316172686C2779469564 @default.
• W2316172686 hasConceptScore W2316172686C2780103800 @default.
• W2316172686 hasConceptScore W2316172686C46699223 @default.
• W2316172686 hasConceptScore W2316172686C502942594 @default.
• W2316172686 hasConceptScore W2316172686C54355233 @default.
• W2316172686 hasConceptScore W2316172686C71315377 @default.
• W2316172686 hasConceptScore W2316172686C86339819 @default.
• W2316172686 hasConceptScore W2316172686C86803240 @default.
• W2316172686 hasLocation W23161726861 @default.
• W2316172686 hasOpenAccess W2316172686 @default.
• W2316172686 hasPrimaryLocation W23161726861 @default.
• W2316172686 hasRelatedWork W17219398 @default.
• W2316172686 hasRelatedWork W24057152 @default.
• W2316172686 hasRelatedWork W2465375 @default.
• W2316172686 hasRelatedWork W25374763 @default.
• W2316172686 hasRelatedWork W34095788 @default.
• W2316172686 hasRelatedWork W40692252 @default.
• W2316172686 hasRelatedWork W6247448 @default.
• W2316172686 hasRelatedWork W8671371 @default.
• W2316172686 hasRelatedWork W9238215 @default.
• W2316172686 hasRelatedWork W9048856 @default.
• W2316172686 isParatext "false" @default.
• W2316172686 isRetracted "false" @default.
• W2316172686 magId "2316172686" @default.
• W2316172686 workType "article" @default.