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- W2316217503 abstract "Conidia of the downy mildew fungus (Peronospora parasitica) which is an obligate parasite were well preserved by freezing at -80°C using suitable cryogenic protectants. The germination rate of the conidia which were suspended in distilled water and frozen at -20°C or -80°C was very low compared with the untreated control. However, the germination rates of the conidia suspended in 5% dimethyl sulfoxide (DMSO) and 5% skim milk were 74.8% and 32.8% of the control, respectively, even after freezing at -20°C. On the other hand, in the preparations directly frozen at -80°C, the germination rate of the conidia decreased markedly in comparison with that after freezing at -20°C. Nevertheless, in the preparations of conidia frozen by the two-step freezing method at -20°C for 24hr followed by -80°C for 24hr, the germination rate remained the same as that in the preparations frozen only at -20°C. During the storage at -20°C, the conidia suspended in 5% DMSO+5% skim milk lost their germination ability after one month. The conidia suspensions which were supplemented with 10% DMSO+5% skim milk or 10% DMSO+10% skim milk and frozen by the two-step method at -20°C and at -80°C, showed a higher germination rate at least up to a period of 12 months. Moreover, these preparations exhibited a high pathogenicity to the broccoli seedlings. Based on these results, it was suggested that the conidia of the broccoli downy mildew fungus treated with protective substances such as 10% DMSO+5% skim milk or 10% DMSO+10% skim milk which were pre-frozen at -20°C for 24hr followed by -80°C could be well preserved for a long period of time." @default.
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- W2316217503 date "1993-01-01" @default.
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- W2316217503 title "Preservation of Conidia of Broccoli Downy Mildew Fungus with Cryogenic Protectants by Freezing at -80.DEG.C.." @default.
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- W2316217503 doi "https://doi.org/10.3186/jjphytopath.59.492" @default.
- W2316217503 hasPublicationYear "1993" @default.
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