FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2316263236> ?p ?o ?g. }

• W2316263236 endingPage "561" @default.
• W2316263236 startingPage "551" @default.
• W2316263236 abstract "Leukotriene A(4) (LTA(4)) is a highly reactive electrophilic intermediate formed during the biosynthesis of the lipid mediators leukotriene B(4) and leukotriene C(4). Deoxynucleosides were found to react as nucleophiles with LTA(4) in aqueous solutions as assessed by UV spectroscopy and electrospray ionization mass spectrometry. Aqueous solutions of native DNA and RNA were also found to react with LTA(4) as assessed by mass spectrometric analysis of the constituent nucleosides derived from enzymatic hydrolysis of the nucleic acids. The most abundant adducts were observed for guanine- and adenine-containing deoxynucleosides and nucleosides. At neutral pH, these reactions led to an overall modification of deoxyguanosine/guanosine residues in DNA and RNA at 15 +/- 1 adducts/10(7) bases and 230 +/- 20 adducts/10(7) bases, respectively, determined by quantitative assay using stable isotope-labeled LTA(4)-nucleoside adduct. An estimation of the relative reactivity of LTA(4) with each of the purine and pyrimidine bases in DNA and RNA was carried out by comparisons of the mass spectral ion abundance of the different adducts (LTA(4)-dAdo, LTA(4)-dCyd, LTA(4)-Thd, LTA(4)-Ado, LTA(4)-Cyd, and LTA(4)-Urd) to the ion signal of known amounts of LTA(4)-dGuo and LTA(4)-Guo standards. The data were corrected for different mass spectrometric response factors that were experimentally determined for each adduct product. The structures of the two most abundant LTA(4)-Guo products were determined by NMR, UV spectroscopy, and mass spectrometry to be 5-hydroxy,12-[Guo-N(2)-yl]-6,8,11,14-eicosatetraenoic acid. Stimulation of human neutrophils with calcium ionophore led to the covalent modification of DNA within the cell as determined by mass spectrometric analysis of lipophilic nucleosides obtained after hydrolysis of extracted DNA. These observations, combined with the intracellular site of 5-lipoxygenase translocation and LTA(4) biosynthesis at the nuclear envelope, suggest that LTA(4) may have access to DNA and RNA within cells and furthermore modify nucleic acids in situ following the activation of 5-lipoxygenase and initiation of LTA(4) biosynthesis." @default.
• W2316263236 created "2016-06-24" @default.
• W2316263236 creator A5037222936 @default.
• W2316263236 creator A5081369599 @default.
• W2316263236 creator A5086931040 @default.
• W2316263236 date "2003-03-28" @default.
• W2316263236 modified "2023-10-09" @default.
• W2316263236 title "Covalent Binding of Leukotriene A₄ to DNA and RNA" @default.
• W2316263236 cites W2012997912 @default.
• W2316263236 cites W2021498593 @default.
• W2316263236 cites W2068657854 @default.
• W2316263236 cites W2077647756 @default.
• W2316263236 cites W2090013289 @default.
• W2316263236 cites W2151426228 @default.
• W2316263236 cites W4250845720 @default.
• W2316263236 cites W627224643 @default.
• W2316263236 doi "https://doi.org/10.1021/tx034018+" @default.
• W2316263236 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12703973" @default.
• W2316263236 hasPublicationYear "2003" @default.
• W2316263236 type Work @default.
• W2316263236 sameAs 2316263236 @default.
• W2316263236 citedByCount "17" @default.
• W2316263236 countsByYear W23162632362012 @default.
• W2316263236 countsByYear W23162632362015 @default.
• W2316263236 countsByYear W23162632362016 @default.
• W2316263236 countsByYear W23162632362020 @default.
• W2316263236 countsByYear W23162632362022 @default.
• W2316263236 countsByYear W23162632362023 @default.
• W2316263236 crossrefType "journal-article" @default.
• W2316263236 hasAuthorship W2316263236A5037222936 @default.
• W2316263236 hasAuthorship W2316263236A5081369599 @default.
• W2316263236 hasAuthorship W2316263236A5086931040 @default.
• W2316263236 hasConcept C104317684 @default.
• W2316263236 hasConcept C108204754 @default.
• W2316263236 hasConcept C162356407 @default.
• W2316263236 hasConcept C178790620 @default.
• W2316263236 hasConcept C185592680 @default.
• W2316263236 hasConcept C24107716 @default.
• W2316263236 hasConcept C2776543447 @default.
• W2316263236 hasConcept C2777995097 @default.
• W2316263236 hasConcept C2778301229 @default.
• W2316263236 hasConcept C2780441211 @default.
• W2316263236 hasConcept C2909011841 @default.
• W2316263236 hasConcept C40684141 @default.
• W2316263236 hasConcept C43617362 @default.
• W2316263236 hasConcept C512185932 @default.
• W2316263236 hasConcept C552990157 @default.
• W2316263236 hasConcept C55493867 @default.
• W2316263236 hasConcept C67705224 @default.
• W2316263236 hasConcept C71240020 @default.
• W2316263236 hasConceptScore W2316263236C104317684 @default.
• W2316263236 hasConceptScore W2316263236C108204754 @default.
• W2316263236 hasConceptScore W2316263236C162356407 @default.
• W2316263236 hasConceptScore W2316263236C178790620 @default.
• W2316263236 hasConceptScore W2316263236C185592680 @default.
• W2316263236 hasConceptScore W2316263236C24107716 @default.
• W2316263236 hasConceptScore W2316263236C2776543447 @default.
• W2316263236 hasConceptScore W2316263236C2777995097 @default.
• W2316263236 hasConceptScore W2316263236C2778301229 @default.
• W2316263236 hasConceptScore W2316263236C2780441211 @default.
• W2316263236 hasConceptScore W2316263236C2909011841 @default.
• W2316263236 hasConceptScore W2316263236C40684141 @default.
• W2316263236 hasConceptScore W2316263236C43617362 @default.
• W2316263236 hasConceptScore W2316263236C512185932 @default.
• W2316263236 hasConceptScore W2316263236C552990157 @default.
• W2316263236 hasConceptScore W2316263236C55493867 @default.
• W2316263236 hasConceptScore W2316263236C67705224 @default.
• W2316263236 hasConceptScore W2316263236C71240020 @default.
• W2316263236 hasIssue "4" @default.
• W2316263236 hasLocation W23162632361 @default.
• W2316263236 hasLocation W23162632362 @default.
• W2316263236 hasOpenAccess W2316263236 @default.
• W2316263236 hasPrimaryLocation W23162632361 @default.
• W2316263236 hasRelatedWork W1525521471 @default.
• W2316263236 hasRelatedWork W1866742045 @default.
• W2316263236 hasRelatedWork W1967835966 @default.
• W2316263236 hasRelatedWork W1969699612 @default.
• W2316263236 hasRelatedWork W1973885375 @default.
• W2316263236 hasRelatedWork W1982696429 @default.
• W2316263236 hasRelatedWork W2029343773 @default.
• W2316263236 hasRelatedWork W2053347593 @default.
• W2316263236 hasRelatedWork W2078231940 @default.
• W2316263236 hasRelatedWork W2089804914 @default.
• W2316263236 hasVolume "16" @default.
• W2316263236 isParatext "false" @default.
• W2316263236 isRetracted "false" @default.
• W2316263236 magId "2316263236" @default.
• W2316263236 workType "article" @default.