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- W2316264927 abstract "Background: The diagnosis of leptomeningeal B-cell malignancies is based on identification of malignant B-cells in the CSF. Molecular analysis of B-cell clonality in CSF samples via amplification and size determination of the highly diverse CDR3-IgH region is a useful approach to distinguish neoplastic from reactive B-cell responses within the CSF and supplements standard diagnostic procedures such as cytology, immuncytochemistry, and flow cytometry. However, the molecular analysis of B-cells using whole CSF specimens is limited if leucemic or lymphomatous spread to the leptomeninges is accompanied by a reactive B-cell response and/or if the total CSF cell count is very low. Here, we applied this technique to assess the clonality of individual cells isolated from standard CSF cytospins using single cell PCR." @default.
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- W2316264927 date "2004-01-01" @default.
- W2316264927 modified "2023-10-16" @default.
- W2316264927 title "Single-cell PCR analysis of the immunoglobulin heavy chain CDR3 region for the diagnosis of leptomeningeal involvement of B-cell malignancies" @default.
- W2316264927 doi "https://doi.org/10.1055/s-2004-833343" @default.
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