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- W2316305034 abstract "There have been several micro-methods in the recent literature for the determination of total iron in blood,1, 2, 3 and it has been suggested that these determinations be used for the calculation of hemoglobin. On account of the constant breakdown of hemoglobin to bilirubin there is a variable quantity of liberated inorganic iron which may amount in certain anemias to more than 5% of the hemoglobin iron.4 Consequently it would seem advisable to make a preliminary separation of inorganic iron from hemoglobin. Riecker4 has observed that such a separation may be accomplished by means of a trichloracetic acid precipitation.In the present study hemoglobin has been calculated from the iron determined in the trichloracetic precipitate from whole blood according to the following technique: A 1 cc. sample of blood is transferred to a 50 cc. centrifuge tube, baked with N/10 HCl and precipitated with trichloracetic acid. After centrifugation the supernatant fluid is poured off, the precipitate dissolved in a minimal..." @default.
- W2316305034 created "2016-06-24" @default.
- W2316305034 creator A5032282131 @default.
- W2316305034 date "1932-06-01" @default.
- W2316305034 modified "2023-09-25" @default.
- W2316305034 title "Estimation of Hemoglobin on a Basis of Protein Iron" @default.
- W2316305034 doi "https://doi.org/10.3181/00379727-29-6258" @default.
- W2316305034 hasPublicationYear "1932" @default.
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