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• W2316320846 abstract "Protein function, structure, and dynamics are intricately correlated, but studies on structure-activity relationships are still only rarely complemented by a detailed analysis of dynamics related to function (functional dynamics). Here, we have applied NMR to investigate the functional dynamics in two homologous periplasmic sugar binding proteins with bidomain composition: Escherichia coli glucose/galactose (GGBP) and ribose (RBP) binding proteins. In contrast to their structural and functional similarity, we observe a remarkable difference in functional dynamics: For RBP, the absence of segmental motions allows only for isolated structural adaptations upon carbohydrate binding in line with an induced fit mechanism; on the other hand, GGBP shows extensive segmental mobility in both apo and holo states, enabling selection of the most favorable conformation upon carbohydrate binding in line with a population shift mechanism. Collective segmental motions are controlled by the hinge composition: by swapping two identified key residues between RBP and GGBP we also interchange their segmental hinge mobility, and the doubly mutated GGBP* no longer experiences changes in conformational entropy upon ligand binding while the complementary RBP* shows the segmental dynamics observed in wild-type GGBP. Most importantly, the segmental interdomain dynamics always increase the apparent substrate affinity and thus, are functional, underscoring the allosteric control that the hinge region exerts on ligand binding." @default.
• W2316320846 created "2016-06-24" @default.
• W2316320846 creator A5006440138 @default.
• W2316320846 creator A5019077821 @default.
• W2316320846 creator A5054255043 @default.
• W2316320846 creator A5073353138 @default.
• W2316320846 date "2012-11-19" @default.
• W2316320846 modified "2023-10-17" @default.
• W2316320846 title "Carbohydrate Affinity for the Glucose–Galactose Binding Protein Is Regulated by Allosteric Domain Motions" @default.
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• W2316320846 doi "https://doi.org/10.1021/ja3092938" @default.
• W2316320846 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23148479" @default.
• W2316320846 hasPublicationYear "2012" @default.
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