FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2316370871> ?p ?o ?g. }

• W2316370871 endingPage "75" @default.
• W2316370871 startingPage "63" @default.
• W2316370871 abstract "Maltose-binding protein (MBP) is a periplasmic binding protein found in Gram negative bacteria. MBP is involved in maltose transport and bacterial chemotaxis; it binds to maltose and maltodextrins comprising α(1-4)-glucosidically linked linear glucose polymers and α(1-4)-glucosidically linked cyclodextrins. Upon ligand binding, MBP changes its conformation from an open to a closed form. This molecular recognition-transducing a ligand-binding event into a physical one-renders MBP an ideal candidate for biosensor development. Here, we describe the construction of a Strep-tag II mutant MBP for reagentless fluorescence sensing. malE, which encodes MBP, was amplified. A cysteine residue was introduced by site-directed mutagenesis to ensure a single label attachment at a specific site with a thiol-specific fluorescent probe. An environmentally sensitive fluorophore (IANBD amide) was covalently attached to the introduced thiol group and analysed by fluorescence sensing. The tagged mutant MBP (D95C) was purified (molecular size, ∼42 kDa). The fluorescence measurements of the IANBD-labelled Strep-tag II-D95C in the solution phase showed an appreciable change in fluorescence intensity (dissociation constant, 7.6±1.75 μM). Our mutant MBP retains maltose-binding activity and is suitable for reagentless fluorescence sensing. Protein pengikat maltosa (MBP) adalah protein pengikat periplasmik yang boleh dijumpai di dalam bakteria Gram negatif. MBP terlibat di dalam proses pengangkutan maltosa serta kemotaksis bakteria; MBP mengikat pada maltosa serta maltodekstrin yang mempunyai rangkaian linear α(1–4)-glukosilik polimer glukosa dan rangkaian siklodekstrin α(1–4)-glukosilik. Semasa protein mengikat pada ligan, perubahan struktur konformasi MBP berlaku dari bentuk terbuka ke bentuk tertutup. Pengecaman molekular ini yang menyebabkan transduksi pengikatan ligan kepada bentuk fizikal menjadikan MBP calon yang sesuai sebagai penderia pendaflour tanpa deria. Di sini, kami akan menerangkan bagaimana mutan Strep-tag II MBP dibina untuk dijadikan sebagai penderia pendaflour tanpa reagen. Gen malE, yang mengekodkan MBP telah diamplifikasikan. Residu sistina telah diperkenalkan melalui proses tapak terarah mutagenesis agar label lekatan tunggal pada tapak yang spesifik yang mempunyai kuar pendaflour spesifik-tiol. Fluorofor yang sensitif pada persekitaran (IANBD amide) terlekat secara kovalen pada kumpulan tiol yang diperkenalkan sebelum ini dan kemudian dianalisa secara deria pendaflour. Mutan MBP (D95C) telah ditulenkan (saiz molekular, ∼42 kDa). Ukuran pendaflour Strep-tag II–D95C yang telah dilabel oleh IANBD di dalam larutan telah menunjukkan perubahan keamatan pendaflour yang memberangsangkan (pemalar penguraian Kd 7.6±1.75 μM). Mutan MBP berjaya mengekalkan aktiviti pengikatan kepada maltosa dan sesuai dijadikan sebagai penderia pendaflour tanpa reagen." @default.
• W2316370871 created "2016-06-24" @default.
• W2316370871 creator A5002904265 @default.
• W2316370871 creator A5007911669 @default.
• W2316370871 creator A5066138252 @default.
• W2316370871 creator A5069462633 @default.
• W2316370871 creator A5091773566 @default.
• W2316370871 date "2016-02-01" @default.
• W2316370871 modified "2023-09-23" @default.
• W2316370871 title "Strep-tag II Mutant Maltose-binding Protein for Reagentless Fluorescence Sensing." @default.
• W2316370871 cites W1547856877 @default.
• W2316370871 cites W1593975599 @default.
• W2316370871 cites W1594136944 @default.
• W2316370871 cites W1972784441 @default.
• W2316370871 cites W1972922718 @default.
• W2316370871 cites W2011562596 @default.
• W2316370871 cites W2013043601 @default.
• W2316370871 cites W2026190471 @default.
• W2316370871 cites W2030814128 @default.
• W2316370871 cites W2047083065 @default.
• W2316370871 cites W2049409642 @default.
• W2316370871 cites W2052044284 @default.
• W2316370871 cites W2059390568 @default.
• W2316370871 cites W2133131542 @default.
• W2316370871 cites W2153237179 @default.
• W2316370871 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4807963" @default.
• W2316370871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27019682" @default.
• W2316370871 hasPublicationYear "2016" @default.
• W2316370871 type Work @default.
• W2316370871 sameAs 2316370871 @default.
• W2316370871 citedByCount "0" @default.
• W2316370871 crossrefType "journal-article" @default.
• W2316370871 hasAuthorship W2316370871A5002904265 @default.
• W2316370871 hasAuthorship W2316370871A5007911669 @default.
• W2316370871 hasAuthorship W2316370871A5066138252 @default.
• W2316370871 hasAuthorship W2316370871A5069462633 @default.
• W2316370871 hasAuthorship W2316370871A5091773566 @default.
• W2316370871 hasConcept C104317684 @default.
• W2316370871 hasConcept C123894998 @default.
• W2316370871 hasConcept C12554922 @default.
• W2316370871 hasConcept C181199279 @default.
• W2316370871 hasConcept C185592680 @default.
• W2316370871 hasConcept C2779201268 @default.
• W2316370871 hasConcept C2780676925 @default.
• W2316370871 hasConcept C40767141 @default.
• W2316370871 hasConcept C55493867 @default.
• W2316370871 hasConcept C86803240 @default.
• W2316370871 hasConceptScore W2316370871C104317684 @default.
• W2316370871 hasConceptScore W2316370871C123894998 @default.
• W2316370871 hasConceptScore W2316370871C12554922 @default.
• W2316370871 hasConceptScore W2316370871C181199279 @default.
• W2316370871 hasConceptScore W2316370871C185592680 @default.
• W2316370871 hasConceptScore W2316370871C2779201268 @default.
• W2316370871 hasConceptScore W2316370871C2780676925 @default.
• W2316370871 hasConceptScore W2316370871C40767141 @default.
• W2316370871 hasConceptScore W2316370871C55493867 @default.
• W2316370871 hasConceptScore W2316370871C86803240 @default.
• W2316370871 hasIssue "1" @default.
• W2316370871 hasLocation W23163708711 @default.
• W2316370871 hasOpenAccess W2316370871 @default.
• W2316370871 hasPrimaryLocation W23163708711 @default.
• W2316370871 hasRelatedWork W1489106209 @default.
• W2316370871 hasRelatedWork W1490002405 @default.
• W2316370871 hasRelatedWork W1964804977 @default.
• W2316370871 hasRelatedWork W1969950519 @default.
• W2316370871 hasRelatedWork W2006572288 @default.
• W2316370871 hasRelatedWork W2012519637 @default.
• W2316370871 hasRelatedWork W2044612312 @default.
• W2316370871 hasRelatedWork W2112431316 @default.
• W2316370871 hasRelatedWork W2408164619 @default.
• W2316370871 hasRelatedWork W2953250471 @default.
• W2316370871 hasVolume "27" @default.
• W2316370871 isParatext "false" @default.
• W2316370871 isRetracted "false" @default.
• W2316370871 magId "2316370871" @default.
• W2316370871 workType "article" @default.