Matches in SemOpenAlex for { <https://semopenalex.org/work/W2316402810> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2316402810 abstract "Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FLEpigenetic processes control the binary on-off states of specific gene sets, thereby creating heritable transcription patterns that drive development, and maintain cellular identity. The prominent epigenetic regulatory marks on eukaryotic chromatin are histone modifications and DNA cytosine methylation (5meCpG). These marks are placed by protein complexes, including members of the histone modifying and DNA methyltransferase (DNMT) enzyme families. It is hypothesized that errors in placement, removal, or reading of epigenetic marks can cause human disease through inappropriate silencing of specific genes. As the epigenetic marks that mediate gene silencing are reversible, there is interest in devising therapeutic strategies to reactivate epigenetically silent genes. Inhibitors of DNA methyltransferase (DNMT) and histone deacetylase (HDAC) enzyme families can reverse epigenetic silencing and produce anti-tumor effects, possibly through reactivation of silent tumor suppressor genes (so-called epigenetic therapy). As these inhibitors show limited specificity within enzyme families, their precise mechanisms of action are not well understood.To identify cellular factors involved in maintenance of epigenetic silencing, we constructed a population of human cells harboring epigenetically silent GFP reporter genes. Using this cell population we have implemented a GFP reporter-based siRNA knockdown screen to identify novel factors and networks that maintain epigenetic silencing in human cells. We have now completed a genome-wide, high throughput siRNA-based screen (21,122 siRNA targets). The screen has produced 128 gene hits that have satisfied several validating criteria. The output of this screen was of high quality, as several of the factors were identified in an earlier independent screen. Among the newly identified hits, there was a significant enrichment for factors that mediate SUMOylation, as well as 25 factors corresponding to novel genes, including 9 with no predicted functions.This screen has the potential to identify novel cellular pathways and reveal new targets for epigenetic therapy of cancer and other diseases.Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3008. doi:10.1158/1538-7445.AM2011-3008" @default.
- W2316402810 created "2016-06-24" @default.
- W2316402810 creator A5020259851 @default.
- W2316402810 creator A5022006116 @default.
- W2316402810 creator A5072055335 @default.
- W2316402810 creator A5084801786 @default.
- W2316402810 date "2011-04-15" @default.
- W2316402810 modified "2023-09-26" @default.
- W2316402810 title "Abstract 3008: Genome-wide siRNA screening identifies epigenetic silencing factor networks as potential targets for cancer therapy" @default.
- W2316402810 doi "https://doi.org/10.1158/1538-7445.am2011-3008" @default.
- W2316402810 hasPublicationYear "2011" @default.
- W2316402810 type Work @default.
- W2316402810 sameAs 2316402810 @default.
- W2316402810 citedByCount "0" @default.
- W2316402810 crossrefType "proceedings-article" @default.
- W2316402810 hasAuthorship W2316402810A5020259851 @default.
- W2316402810 hasAuthorship W2316402810A5022006116 @default.
- W2316402810 hasAuthorship W2316402810A5072055335 @default.
- W2316402810 hasAuthorship W2316402810A5084801786 @default.
- W2316402810 hasConcept C104317684 @default.
- W2316402810 hasConcept C114691636 @default.
- W2316402810 hasConcept C119056186 @default.
- W2316402810 hasConcept C121912465 @default.
- W2316402810 hasConcept C150194340 @default.
- W2316402810 hasConcept C150425827 @default.
- W2316402810 hasConcept C190727270 @default.
- W2316402810 hasConcept C2780737395 @default.
- W2316402810 hasConcept C33288867 @default.
- W2316402810 hasConcept C41091548 @default.
- W2316402810 hasConcept C54355233 @default.
- W2316402810 hasConcept C63016187 @default.
- W2316402810 hasConcept C64927066 @default.
- W2316402810 hasConcept C83640560 @default.
- W2316402810 hasConcept C86803240 @default.
- W2316402810 hasConcept C91965660 @default.
- W2316402810 hasConceptScore W2316402810C104317684 @default.
- W2316402810 hasConceptScore W2316402810C114691636 @default.
- W2316402810 hasConceptScore W2316402810C119056186 @default.
- W2316402810 hasConceptScore W2316402810C121912465 @default.
- W2316402810 hasConceptScore W2316402810C150194340 @default.
- W2316402810 hasConceptScore W2316402810C150425827 @default.
- W2316402810 hasConceptScore W2316402810C190727270 @default.
- W2316402810 hasConceptScore W2316402810C2780737395 @default.
- W2316402810 hasConceptScore W2316402810C33288867 @default.
- W2316402810 hasConceptScore W2316402810C41091548 @default.
- W2316402810 hasConceptScore W2316402810C54355233 @default.
- W2316402810 hasConceptScore W2316402810C63016187 @default.
- W2316402810 hasConceptScore W2316402810C64927066 @default.
- W2316402810 hasConceptScore W2316402810C83640560 @default.
- W2316402810 hasConceptScore W2316402810C86803240 @default.
- W2316402810 hasConceptScore W2316402810C91965660 @default.
- W2316402810 hasLocation W23164028101 @default.
- W2316402810 hasOpenAccess W2316402810 @default.
- W2316402810 hasPrimaryLocation W23164028101 @default.
- W2316402810 hasRelatedWork W182854632 @default.
- W2316402810 hasRelatedWork W1833062828 @default.
- W2316402810 hasRelatedWork W1987487060 @default.
- W2316402810 hasRelatedWork W1989573122 @default.
- W2316402810 hasRelatedWork W1996431377 @default.
- W2316402810 hasRelatedWork W2031992157 @default.
- W2316402810 hasRelatedWork W2038862787 @default.
- W2316402810 hasRelatedWork W2092293354 @default.
- W2316402810 hasRelatedWork W2093011599 @default.
- W2316402810 hasRelatedWork W2109861500 @default.
- W2316402810 hasRelatedWork W2117856901 @default.
- W2316402810 hasRelatedWork W2141792938 @default.
- W2316402810 hasRelatedWork W2147934734 @default.
- W2316402810 hasRelatedWork W2206573665 @default.
- W2316402810 hasRelatedWork W2292819684 @default.
- W2316402810 hasRelatedWork W2502565809 @default.
- W2316402810 hasRelatedWork W2521333054 @default.
- W2316402810 hasRelatedWork W2556294237 @default.
- W2316402810 hasRelatedWork W3125996539 @default.
- W2316402810 hasRelatedWork W2959562195 @default.
- W2316402810 isParatext "false" @default.
- W2316402810 isRetracted "false" @default.
- W2316402810 magId "2316402810" @default.
- W2316402810 workType "article" @default.