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- W2316409623 abstract "A fluorescence in situ hybridization (FISH) technique using 16S rRNA-targeted oligonucleotide probes was developed for rapid detection of microorganisms for use in the microbial enhancement of oil recovery (MEOR) process. Two microorganisms, Enterobacter cloacae TRC-322 and Bacillus licheniformis TRC-18-2-a, were selected from a collection of Enterobacter sp. and Bacillus sp. which were screened in previous studies as candidate microorganisms for injection, and were used for this experiment. Oligonucleotide probes, design based on specific sequences in the 16S rRNA gene were labeled with either fluorescein isothiocianate (FITC), or 6-car-boxy-X-rhodamine (ROX), and were allowed to hybridize with fixed cells of the two microorganisms noted above. The fluorescence signal emitted from each microorganism cells could clearly be detected by an epifluorescence microscope. Moreover, E. cloacae TRC-322 and B. licheniformis TRC-18-2-a, suspended in actual reservoir brine, including inorganic salts, oil and aboriginal cells of the reservoir brine, could be detected directly by this hybridization method, without the need for cultivation and isolation." @default.
- W2316409623 created "2016-06-24" @default.
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- W2316409623 date "2000-01-01" @default.
- W2316409623 modified "2023-10-12" @default.
- W2316409623 title "Identification of Bacteria Used for Microbial Enhanced Oil Recovery Process by Fluorescence In Situ Hybridization Technique." @default.
- W2316409623 cites W2025433848 @default.
- W2316409623 doi "https://doi.org/10.1627/jpi1958.43.43" @default.
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