Matches in SemOpenAlex for { <https://semopenalex.org/work/W2316498841> ?p ?o ?g. }
- W2316498841 endingPage "487" @default.
- W2316498841 startingPage "477" @default.
- W2316498841 abstract "Lysosomal α-D-mannosidase is an exoglycosidase involved in the ordered degradation of N-linked oligosaccharides. It is ubiquitously expressed, although the main transcript is more abundant in peripheral blood leucocytes. Here we report that α-D-mannosidase enzyme activity is very high in the promyelocytic leukaemia cell lines HL60 and NB4, as compared with other leukaemic cell lines or cells from different human sources. The MAN2B1 transcript level correlates with enzyme activity, indicating a transcriptional up-regulation of the α-D-mannosidase gene. The promoter was then characterized in HEK-293 cells (human embryonic kidney 293 cells) and HL60 cells; regulatory sequences crucial for its activity were determined by reporter gene assay in HEK-293 cells and located in the region -101/-71 with respect to the first ATG codon. Supershift assay demonstrated that Sp1 (specificity protein 1) bound to this sequence both in HEK-293 and HL60 cells. However, 5'-RACE (5'-rapid amplification of cDNA ends) indicated the use of multiple upstream TSSs (transcription start sites) in HL60 with respect to HEK-293 cells and gel shift analysis of the sequence -373/-269 demonstrated a specific binding by NF-κB (nuclear factor κB) transcription factor in HL60 but not in HEK-293 cells. We concluded that despite the α-D-mannosidase promoter showing typical features of housekeeping gene promoters, α-D-mannosidase transcription is specifically regulated in HL60 by NF-κB transcription factor." @default.
- W2316498841 created "2016-06-24" @default.
- W2316498841 creator A5017383179 @default.
- W2316498841 creator A5017808610 @default.
- W2316498841 creator A5071609016 @default.
- W2316498841 creator A5075781885 @default.
- W2316498841 creator A5080494751 @default.
- W2316498841 creator A5081542814 @default.
- W2316498841 creator A5084685210 @default.
- W2316498841 date "2011-09-15" @default.
- W2316498841 modified "2023-09-25" @default.
- W2316498841 title "Human lysosomal α-<scp>D</scp>-mannosidase regulation in promyelocytic leukaemia cells" @default.
- W2316498841 cites W1504924831 @default.
- W2316498841 cites W1526419517 @default.
- W2316498841 cites W1564896848 @default.
- W2316498841 cites W1581923494 @default.
- W2316498841 cites W1589900156 @default.
- W2316498841 cites W1968807526 @default.
- W2316498841 cites W1977687076 @default.
- W2316498841 cites W1983805610 @default.
- W2316498841 cites W1984498233 @default.
- W2316498841 cites W1988356885 @default.
- W2316498841 cites W1989975778 @default.
- W2316498841 cites W1990634720 @default.
- W2316498841 cites W1994338782 @default.
- W2316498841 cites W2008545486 @default.
- W2316498841 cites W2024953714 @default.
- W2316498841 cites W2028926394 @default.
- W2316498841 cites W2032819570 @default.
- W2316498841 cites W2035474025 @default.
- W2316498841 cites W2039102735 @default.
- W2316498841 cites W2041639550 @default.
- W2316498841 cites W2043634641 @default.
- W2316498841 cites W2051994734 @default.
- W2316498841 cites W2054679377 @default.
- W2316498841 cites W2058356488 @default.
- W2316498841 cites W2058560110 @default.
- W2316498841 cites W2059419250 @default.
- W2316498841 cites W2070246465 @default.
- W2316498841 cites W2070672342 @default.
- W2316498841 cites W2079463341 @default.
- W2316498841 cites W2082771996 @default.
- W2316498841 cites W2085911679 @default.
- W2316498841 cites W2086254726 @default.
- W2316498841 cites W2089126365 @default.
- W2316498841 cites W2094966616 @default.
- W2316498841 cites W2124983243 @default.
- W2316498841 cites W2127392617 @default.
- W2316498841 cites W2139100711 @default.
- W2316498841 cites W2158438223 @default.
- W2316498841 cites W2202227433 @default.
- W2316498841 cites W2401743386 @default.
- W2316498841 cites W2414186762 @default.
- W2316498841 cites W40166978 @default.
- W2316498841 cites W4293247451 @default.
- W2316498841 cites W432228268 @default.
- W2316498841 doi "https://doi.org/10.1042/bsr20110020" @default.
- W2316498841 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21521175" @default.
- W2316498841 hasPublicationYear "2011" @default.
- W2316498841 type Work @default.
- W2316498841 sameAs 2316498841 @default.
- W2316498841 citedByCount "5" @default.
- W2316498841 countsByYear W23164988412014 @default.
- W2316498841 countsByYear W23164988412016 @default.
- W2316498841 countsByYear W23164988412022 @default.
- W2316498841 crossrefType "journal-article" @default.
- W2316498841 hasAuthorship W2316498841A5017383179 @default.
- W2316498841 hasAuthorship W2316498841A5017808610 @default.
- W2316498841 hasAuthorship W2316498841A5071609016 @default.
- W2316498841 hasAuthorship W2316498841A5075781885 @default.
- W2316498841 hasAuthorship W2316498841A5080494751 @default.
- W2316498841 hasAuthorship W2316498841A5081542814 @default.
- W2316498841 hasAuthorship W2316498841A5084685210 @default.
- W2316498841 hasConcept C101762097 @default.
- W2316498841 hasConcept C104317684 @default.
- W2316498841 hasConcept C108625454 @default.
- W2316498841 hasConcept C150194340 @default.
- W2316498841 hasConcept C153911025 @default.
- W2316498841 hasConcept C174510640 @default.
- W2316498841 hasConcept C2777174400 @default.
- W2316498841 hasConcept C2778903051 @default.
- W2316498841 hasConcept C54355233 @default.
- W2316498841 hasConcept C55493867 @default.
- W2316498841 hasConcept C81885089 @default.
- W2316498841 hasConcept C86339819 @default.
- W2316498841 hasConcept C86803240 @default.
- W2316498841 hasConcept C95444343 @default.
- W2316498841 hasConceptScore W2316498841C101762097 @default.
- W2316498841 hasConceptScore W2316498841C104317684 @default.
- W2316498841 hasConceptScore W2316498841C108625454 @default.
- W2316498841 hasConceptScore W2316498841C150194340 @default.
- W2316498841 hasConceptScore W2316498841C153911025 @default.
- W2316498841 hasConceptScore W2316498841C174510640 @default.
- W2316498841 hasConceptScore W2316498841C2777174400 @default.
- W2316498841 hasConceptScore W2316498841C2778903051 @default.
- W2316498841 hasConceptScore W2316498841C54355233 @default.
- W2316498841 hasConceptScore W2316498841C55493867 @default.
- W2316498841 hasConceptScore W2316498841C81885089 @default.