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- W2316660129 abstract "MMTV‐Wnt‐1 transgenic mice develop heterogeneous mammary tumors that accurately recapitulate human basal‐like breast cancer. Additionally, targeted alterations in the Wnt signaling pathway in mammary epithelial cells preferentially induce mammary cancers from progenitor cells. Therefore, we hypothesized that MMTV‐Wnt‐1 mammary tumors harbor cells with cancer stem cell characteristics. To test this hypothesis, we characterized (in vitro and in vivo) two newly generated, clonal mammary epithelial cell lines (WA4 and WG4) from a MMTV‐Wnt‐1 transgenic mouse tumor. The cell lines were analyzed using flow cytometry for CD44 and CD24 cell surface markers (a CD44+/CD24− profile is indicative of putative human and murine mammary cancer stem cells), mammosphere formation (low density seeding on non‐adherent plates in stem‐cell media), in vitro scratch assay (to measure wound healing), and by an in vitro Matrigel invasion chamber assay (to quantitate cell invasion). Finally, unsorted WA4 and WG4 cell lines were transplanted into syngeneic mammary fat pads of C57BL/6 mice (1×10^7 cells/mouse; n=5/cell line), and tumor burden was assessed after 3 weeks. Flow cytometric analysis demonstrated that the percentage of CD44+/CD24− cells was 62.2% in the WA4 line and 2.4% in the WG4 line. Serum starvation enriched the WA4 line for CD44+/CD24− cells (from 62.2% to 82.0%), without altering the composition of the WG4 line. Approximately 30% of unsorted WA4 cells, but In vivo , the WA4 cell line was associated with larger, more aggressive tumors on average (+SD) than the WG4 line (2.09 +/−0.68 g and 0.03 +/−0.02 g, respectively, p Citation Information: Cancer Prev Res 2010;3(1 Suppl):B66." @default.
- W2316660129 created "2016-06-24" @default.
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- W2316660129 date "2010-01-07" @default.
- W2316660129 modified "2023-09-25" @default.
- W2316660129 title "Abstract B66: Aggressiveness of MMTV‐Wnt‐1 mammary tumors corresponds with enrichment of a CD44+/CD24− putative cancer stem cell population" @default.
- W2316660129 doi "https://doi.org/10.1158/1940-6207.prev-09-b66" @default.
- W2316660129 hasPublicationYear "2010" @default.
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