FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2316669803> ?p ?o ?g. }

• W2316669803 endingPage "336" @default.
• W2316669803 startingPage "327" @default.
• W2316669803 abstract "Translational coupling is the interdependence of translation efficiency of neighboring genes encoded within an operon. The degree of coupling may be quantified by measuring how the translation rate of a gene is modulated by the translation rate of its upstream gene. Translational coupling was observed in prokaryotic operons several decades ago, but the quantitative range of modulation translational coupling leads to and the factors governing this modulation were only partially characterized. In this study, we systematically quantify and characterize translational coupling in E. coli synthetic operons using a library of plasmids carrying fluorescent reporter genes that are controlled by a set of different ribosome binding site (RBS) sequences. The downstream gene expression level is found to be enhanced by the upstream gene expression via translational coupling with the enhancement level varying from almost no coupling to over 10-fold depending on the upstream gene’s sequence. Additionally, we find that the level of translational coupling in our system is similar between the second and third locations in the operon. The coupling depends on the distance between the stop codon of the upstream gene and the start codon of the downstream gene. This study is the first to systematically and quantitatively characterize translational coupling in a synthetic E. coli operon. Our analysis will be useful in accurate manipulation of gene expression in synthetic biology and serves as a step toward understanding the mechanisms involved in translational expression modulation." @default.
• W2316669803 created "2016-06-24" @default.
• W2316669803 creator A5006038361 @default.
• W2316669803 creator A5012297560 @default.
• W2316669803 creator A5021074279 @default.
• W2316669803 creator A5024451873 @default.
• W2316669803 creator A5032409006 @default.
• W2316669803 creator A5049473553 @default.
• W2316669803 creator A5075456153 @default.
• W2316669803 creator A5081594451 @default.
• W2316669803 date "2013-04-16" @default.
• W2316669803 modified "2023-09-30" @default.
• W2316669803 title "Quantifying Translational Coupling in <i>E. coli</i> Synthetic Operons Using RBS Modulation and Fluorescent Reporters" @default.
• W2316669803 cites W1492472480 @default.
• W2316669803 cites W1585145369 @default.
• W2316669803 cites W1610918399 @default.
• W2316669803 cites W1969737118 @default.
• W2316669803 cites W1970625978 @default.
• W2316669803 cites W1972522740 @default.
• W2316669803 cites W1976257434 @default.
• W2316669803 cites W1978758498 @default.
• W2316669803 cites W2001426985 @default.
• W2316669803 cites W2002227368 @default.
• W2316669803 cites W2014766112 @default.
• W2316669803 cites W2020044960 @default.
• W2316669803 cites W2030680112 @default.
• W2316669803 cites W2032480477 @default.
• W2316669803 cites W2034717680 @default.
• W2316669803 cites W2038745896 @default.
• W2316669803 cites W2041913199 @default.
• W2316669803 cites W2044366645 @default.
• W2316669803 cites W2047360542 @default.
• W2316669803 cites W2047882868 @default.
• W2316669803 cites W2050783586 @default.
• W2316669803 cites W2051242197 @default.
• W2316669803 cites W2076425379 @default.
• W2316669803 cites W2080233018 @default.
• W2316669803 cites W2083532639 @default.
• W2316669803 cites W2091731123 @default.
• W2316669803 cites W2106913956 @default.
• W2316669803 cites W2158473123 @default.
• W2316669803 cites W2160463360 @default.
• W2316669803 cites W2170851716 @default.
• W2316669803 doi "https://doi.org/10.1021/sb400002n" @default.
• W2316669803 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23654261" @default.
• W2316669803 hasPublicationYear "2013" @default.
• W2316669803 type Work @default.
• W2316669803 sameAs 2316669803 @default.
• W2316669803 citedByCount "95" @default.
• W2316669803 countsByYear W23166698032013 @default.
• W2316669803 countsByYear W23166698032014 @default.
• W2316669803 countsByYear W23166698032015 @default.
• W2316669803 countsByYear W23166698032016 @default.
• W2316669803 countsByYear W23166698032017 @default.
• W2316669803 countsByYear W23166698032018 @default.
• W2316669803 countsByYear W23166698032019 @default.
• W2316669803 countsByYear W23166698032020 @default.
• W2316669803 countsByYear W23166698032021 @default.
• W2316669803 countsByYear W23166698032022 @default.
• W2316669803 countsByYear W23166698032023 @default.
• W2316669803 crossrefType "journal-article" @default.
• W2316669803 hasAuthorship W2316669803A5006038361 @default.
• W2316669803 hasAuthorship W2316669803A5012297560 @default.
• W2316669803 hasAuthorship W2316669803A5021074279 @default.
• W2316669803 hasAuthorship W2316669803A5024451873 @default.
• W2316669803 hasAuthorship W2316669803A5032409006 @default.
• W2316669803 hasAuthorship W2316669803A5049473553 @default.
• W2316669803 hasAuthorship W2316669803A5075456153 @default.
• W2316669803 hasAuthorship W2316669803A5081594451 @default.
• W2316669803 hasConcept C104317684 @default.
• W2316669803 hasConcept C105580179 @default.
• W2316669803 hasConcept C131584629 @default.
• W2316669803 hasConcept C149364088 @default.
• W2316669803 hasConcept C150194340 @default.
• W2316669803 hasConcept C161733203 @default.
• W2316669803 hasConcept C165864922 @default.
• W2316669803 hasConcept C170344550 @default.
• W2316669803 hasConcept C176990463 @default.
• W2316669803 hasConcept C191897082 @default.
• W2316669803 hasConcept C192562407 @default.
• W2316669803 hasConcept C203075996 @default.
• W2316669803 hasConcept C22744801 @default.
• W2316669803 hasConcept C2775992057 @default.
• W2316669803 hasConcept C39685955 @default.
• W2316669803 hasConcept C4718897 @default.
• W2316669803 hasConcept C54355233 @default.
• W2316669803 hasConcept C547475151 @default.
• W2316669803 hasConcept C70721500 @default.
• W2316669803 hasConcept C86803240 @default.
• W2316669803 hasConceptScore W2316669803C104317684 @default.
• W2316669803 hasConceptScore W2316669803C105580179 @default.
• W2316669803 hasConceptScore W2316669803C131584629 @default.
• W2316669803 hasConceptScore W2316669803C149364088 @default.
• W2316669803 hasConceptScore W2316669803C150194340 @default.
• W2316669803 hasConceptScore W2316669803C161733203 @default.
• W2316669803 hasConceptScore W2316669803C165864922 @default.
• W2316669803 hasConceptScore W2316669803C170344550 @default.
• W2316669803 hasConceptScore W2316669803C176990463 @default.

• next